LOCUS       sequence-497752-        1458 bp    DNA     linear   UNA 13-FEB-2026
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            Bacillus sp. YM55-1 derived aspartate lyase in combination with 
            turbo GFP using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1458)
  TITLE     Aspartate availability drives differential engagement of the 
            malate-aspartate shuttle
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 13, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57-GFP-P2A-ByASPase
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1432..1455
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aacacagacg tcaggatcga aaaggatttc ttgggtgaga aagaaatccc caaggatgcc
       61 tactacggag tgcagactat cagggccacc gagaacttcc ccatcacggg ctaccgcatc
      121 caccctgagc tgatcaagtc tcttggtata gtcaagaaga gcgcagctct ggcgaatatg
      181 gaagtagggc tgctagacaa ggaagtgggc cagtacattg tgaaggcagc cgatgaggtt
      241 atcgagggca agtggaacga ccagttcatc gtagatccca tacagggcgg cgcgggcaca
      301 agcataaaca tgaacgccaa cgaggtcata gccaatcgag ccctcgagct gatgggtgaa
      361 gagaagggca attactctaa gatctcaccc aattctcacg tcaacatgag tcagagcacc
      421 aatgacgcct ttccgaccgc tacccacata gccgttttgt cactgctgaa ccagctcata
      481 gagaccacta agtatatgca gcaggagttt atgaagaagg cggacgagtt tgccggagtg
      541 atcaagatgg ggcgcaccca tctgcaggat gccgtcccca tactgctagg tcaggaattc
      601 gaggcctacg caagagtcat cgcaagggac atagagcgca tcgcaaacac acggaataac
      661 ctctatgata ttaatatggg cgctactgct gtagggacag gtcttaacgc cgaccccgag
      721 tacatctcta ttgtgaccga gcacctcgct aagttttccg gtcaccctct tcgctctgcc
      781 cagcacctcg tcgatgccac acagaacacg gattgttaca ccgaggtatc ctctgctctc
      841 aaggtctgta tgataaatat gtcaaagatc gcaaacgacc ttagattgat ggcttccggc
      901 cctcgagccg gtctgagtga gattgtgctc ccagcgagac agcccgggag ctccattatg
      961 cccgggaagg tcaaccccgt catgccagag gtcatgaatc aggtagcctt tcaggttttc
     1021 gggaacgacc ttaccataac cagtgccagt gaggctgggc agttcgagct gaacgtcatg
     1081 gagccagtac ttttctttaa cctgatccag tctatcagca tcatgaccaa cgttttcaag
     1141 tctttcaccg agaattgtct gaagggaatc aaagcgaacg aggagcgaat gaaggagtac
     1201 gtggaaaagt caataggcat tatcacagct atcaatcctc acgttggtta cgagaccgca
     1261 gccaagctag cccgcgaggc ctacctgact ggcgagagca taagggagct ctgtataaaa
     1321 tacggggttc taactgagga gcaactgaac gagattctga acccttacga gatgacgcac
     1381 cctgggatcg ccgggcggaa gggcgggtca ggcggcagtg gcggctccat ggactataaa
     1441 gacgatgacg ataaatag
//
