LOCUS       sequence-497753-        1488 bp    DNA     linear   UNA 02-FEB-2026
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            E.coli derived aspartate lyase in combination with turbo GFP using 
            the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1488)
  TITLE     Aspartate availability drives differential engagement of the 
            malate-aspartate shuttle
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCW57-GFP-P2A-EcASPase
FEATURES             Location/Qualifiers
     source          1..1488
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1462..1485
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tccaataata tcaggataga ggaagacttg ctgggaacaa gagaggtacc tgcagacgct
       61 tattacgggg tgcatacctt gcgggctata gagaatttct acatatctaa taataagata
      121 tccgacatcc ccgagttcgt gcgaggcatg gtcatggtga agaaggctgc tgcaatggcc
      181 aataaggaat tgcagactat cccgaagtct gtggccaacg ctataatcgc agcttgcgac
      241 gaggtactca ataatggtaa gtgtatggac caatttcccg tggatgtgta tcaaggtggg
      301 gcgggcacct cagtcaatat gaatacgaat gaggttctcg cgaacattgg ccttgagctc
      361 atgggacatc agaagggcga gtaccaatat ctcaatccta atgatcacgt gaataagtgc
      421 caaagcacaa atgatgcata tccaacaggc tttcgaatag cggtctattc cagcctaatc
      481 aaacttgttg acgcaataaa tcagcttcgg gaaggattcg agaggaaggc cgtggagttt
      541 caagatatac tcaagatggg cagaactcaa ctccaagatg cggtccccat gacgcttgga
      601 caagagttta gggccttttc aattcttctc aaggaagagg tcaagaatat tcagagaaca
      661 gcagagcttt tgctagaggt aaatctcggg gctactgcta ttggcaccgg actaaatact
      721 cccaaggaat atagcccact cgcggtcaag aagctagccg aggtgacggg atttccctgt
      781 gttccagccg aggatcttat agaggctact agcgattgtg gagcgtacgt catggtgcat
      841 ggagcattga agaggttggc agtaaagatg tctaagattt gcaatgatct ccgactgctg
      901 agcagcgggc ccagagctgg tctcaatgaa attaatctac ctgagctcca agcaggtagc
      961 agtattatgc ctgccaaggt gaatccagtg gtacctgagg ttgtcaatca agtctgtttt
     1021 aaggtgattg ggaatgatac aacggtgaca atggcggccg aggctggaca acttcaactg
     1081 aatgtgatgg aacccgtaat cgggcaagct atgtttgaga gcgtccatat cctaacaaat
     1141 gcatgttata atcttttgga gaagtgtatc aatgggatta cggcaaataa agaggtctgt
     1201 gaggggtatg tctataattc aattggcata gtgacgtatt tgaatccctt tattgggcat
     1261 cataatggcg atattgtagg gaagatttgc gctgagactg gcaaatctgt gcgagaggtt
     1321 gtcttggagc gaggcttgct gacagaggcc gagctggatg acatatttag cgtgcaaaac
     1381 ctcatgcatc ccgcgtataa ggctaagaga tacaccgacg agtccgagca aggtggcagc
     1441 ggtgggtcag gtgggtccat ggactataaa gacgatgacg ataaatga
//
