LOCUS       sequence-497874-        9881 bp    DNA     circular SYN 21-JAN-2026
DEFINITION  Expression of human FUT8 with C-terminal myc and FLAG tags in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9881)
  TITLE     Hypofucosylation promotes pertussis toxin binding to cell surface 
            glycococonjugates and pertussis toxin-induced intracellular ERK 
            signaling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 21, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-FUT8-IRES-puro
FEATURES             Location/Qualifiers
     source          1..9881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(760..1551,1552..1620))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1383..1402
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1621..1725)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1773..1907
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1810..1829)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1864..1883
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2075..2708
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2755..2880
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3377..3610
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3795..3839
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4102..4217
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        4275..4578
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4579..4782
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4733..4753
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4779..4803
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6630..6659
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             6678..6701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(6705..7279,7280..7282,7283..7291)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6872..6889)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7099..7118
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7311..7910
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7311..7330)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7807..7827
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7924..8512
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7977..7997)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8395..8406)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8719..9352
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9480..9496)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9480..9496)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9493..9515)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9504..9520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9528..9534,9535..9552,9553..9558))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9573..9594
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9711..9728)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
      841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
      901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
      961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     1081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     1381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1741 agtgccacct gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt
     1801 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     1861 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     1921 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     1981 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     2041 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     2101 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     2161 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     2221 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     2281 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     2341 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     2401 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     2461 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     2521 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2581 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2641 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2701 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2761 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2821 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2881 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     2941 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3001 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3061 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3121 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3181 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     3241 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3301 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3361 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3421 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3481 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3541 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     3601 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     3661 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     3721 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     3781 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     3841 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     3901 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     3961 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4021 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4081 acggatctcg acggtatcgc ctttaaaaga aaagggggga ttggggggta cagtgcaggg
     4141 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     4201 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttatcgataa
     4261 gcttgggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     4321 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     4381 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     4441 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     4501 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     4561 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     4621 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     4681 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     4741 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
     4801 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctactag
     4861 aggatctatt tccggtgatg cggccatgga ctggttcctg gcgttggatt atgctcattc
     4921 tttttgcctg ggggaccttg ctgttttata taggtggtca cttggtacga gataatgacc
     4981 atcctgatca ctctagccga gaactgtcca agattctggc aaagcttgaa cgcttaaaac
     5041 aacagaatga agacttgagg cgaatggccg aatctctccg gataccagaa ggccctattg
     5101 atcaggggcc agctatagga agagtacgcg ttttagaaga gcagcttgtt aaggccaaag
     5161 aacagattga aaattacaag aaacagacca gaaatggtct ggggaaggat catgaaatcc
     5221 tgaggaggag gattgaaaat ggagctaaag agctctggtt tttcctacag agtgaattga
     5281 agaaattaaa gaacttagaa ggaaatgaac tccaaagaca tgcagatgaa tttcttttgg
     5341 atttaggaca tcatgaaagg tctataatga cggatctata ctacctcagt cagacagatg
     5401 gagcaggtga ttggcgggaa aaagaggcca aagatctgac agaactggtt cagcggagaa
     5461 taacatatct tcagaatccc aaggactgca gcaaagccaa aaagctggtg tgtaatatca
     5521 acaaaggctg tggctatggc tgtcagctcc atcatgtggt ctactgcttc atgattgcat
     5581 atggcaccca gcgaacactc atcttggaat ctcagaattg gcgctatgct actggtggat
     5641 gggagactgt atttaggcct gtaagtgaga catgcacaga cagatctggc atctccactg
     5701 gacactggtc aggtgaagtg aaggacaaaa atgttcaagt ggtcgagctt cccattgtag
     5761 acagtcttca tccccgtcct ccatatttac ccttggctgt accagaagat ctcgcagatc
     5821 gacttgtacg agtgcatggt gaccctgcag tgtggtgggt gtctcagttt gtcaaatact
     5881 tgatccgccc acagccttgg ctagaaaaag aaatagaaga agccaccaag aagcttggct
     5941 tcaaacatcc agttattgga gtccatgtca gacgcacaga caaagtggga acagaagctg
     6001 ccttccatcc cattgaagag tacatggtgc atgttgaaga acattttcag cttcttgcac
     6061 gcagaatgca agtggacaaa aaaagagtgt atttggccac agatgaccct tctttattaa
     6121 aggaggcaaa aacaaagtac cccaattatg aatttattag tgataactct atttcctggt
     6181 cagctggact gcacaatcga tacacagaaa attcacttcg tggagtgatc ctggatatac
     6241 attttctctc tcaggcagac ttcctagtgt gtactttttc atcccaggtc tgtcgagttg
     6301 cttatgaaat tatgcaaaca ctacatcctg atgcctctgc aaacttccat tctttagatg
     6361 acatctacta ttttgggggc cagaatgccc acaatcaaat tgccatttat gctcaccaac
     6421 cccgaactgc agatgaaatt cccatggaac ctggagatat cattggtgtg gctggaaatc
     6481 attgggatgg ctattctaaa ggtgtcaaca ggaaattggg aaggacgggc ctatatccct
     6541 cctacaaagt tcgagagaag atagaaacgg tcaagtaccc cacatatcct gaggctgaga
     6601 aaagcggacc gacgcgtacg cggccgctcg agcagaaact catctcagaa gaggatctgg
     6661 cagcaaatga tatcctggat tacaaggatg acgacgataa gccgcccctc tccctccccc
     6721 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     6781 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6841 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6901 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6961 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     7021 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     7081 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     7141 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     7201 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     7261 tgaaaaacac gatgataagc ttgccacaac ccacaaggag acgaccttcc atgaccgagt
     7321 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     7381 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     7441 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     7501 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     7561 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     7621 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     7681 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     7741 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     7801 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     7861 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag acgcgtctgg
     7921 aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7981 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     8041 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     8101 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     8161 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     8221 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     8281 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     8341 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8401 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8461 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     8521 aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8581 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8641 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     8701 taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     8761 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     8821 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     8881 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     8941 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     9001 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     9061 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     9121 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     9181 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     9241 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     9301 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     9361 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     9421 aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc gtaatcatgg
     9481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt t
//
