LOCUS       sequence-49791-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, 
            Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, 
            Hannon GJ, Lowe SW
  TITLE     A rapid and scalable system for studying gene function in mice using
            conditional RNA interference.
  JOURNAL   Cell. 2011 Apr 1;145(1):145-58.
  PUBMED    21458673
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-04-1"; volume: "145(1)"; pages: "145-58"
COMMENT     Sequence Label: pCol-TGM
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..106
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    join(139..204,205..242)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     polyA_signal    411..545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    665..799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    811..829
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    853..871
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    895..913
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 tccgggcctt tcgacctgca tccatctaga tctcgatcga gcagctgaag cttatggaga
       61 agttactatt ccgaagttcc tattctctag aaagtatagg aacttcgatc cccctcgatc
      121 ccccgggctg cagatctgta gggcgcagta gtccagggtt tccttgatga tgtcatactt
      181 atcctgtccc ttttttttcc acagctcgcg gttgaggaca aactcttcgc ggtctttcca
      241 gtggggatcg acggtatcga taagcttgca gatctgcgac tctagaggat ctgcgactct
      301 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      361 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
      421 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      481 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      541 ggatctgcga ctctagagga tcataatcag ccataccaca tttgtagagg ttttacttgc
      601 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
      661 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
      721 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
      781 atcttatcat gtctggatcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      841 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
      901 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat a
//
