LOCUS       sequence-49796-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Premsrirut PK, Dow LE, Kim SY, Camiolo M, Malone CD, Miething C, 
            Scuoppo C, Zuber J, Dickins RA, Kogan SC, Shroyer KR, Sordella R, 
            Hannon GJ, Lowe SW
  TITLE     A rapid and scalable system for studying gene function in mice using
            conditional RNA interference.
  JOURNAL   Cell. 2011 Apr 1;145(1):145-58.
  PUBMED    21458673
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-04-1"; volume: "145(1)"; pages: "145-58"
COMMENT     Sequence Label: pTGMP
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           53..146
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           164..276
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(325..348)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 aggagactgt acaagtaatg aaccggtcga ctagggataa cagggtaatt gtttgaatga
       61 ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc tgggattact
      121 tcttcaggtt aacccaacag aaggctcgag caagcagtca gaattcaagg ggctacttta
      181 ggagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac
      241 aaagctgaat taaaatggta taaattaaat cacttttttc aattctaccg ggtaggggag
      301 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      361 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      421 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      481 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      541 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      601 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
      661 ggcgggctca
//
