LOCUS       sequence-498179-        8371 bp    DNA     circular SYN 26-JAN-2026
DEFINITION  In vitro transcription (SP6 promotor) or mammalian expression (CMV 
            promotor) of human Pum2 fused to the catalytic domain of zebrafish 
            Adar1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8371)
  TITLE     Cifuentes Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2_hsPum2_dreAdar1
FEATURES             Location/Qualifiers
     source          1..8371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3136..3162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    4423..4557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4447..4466)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4501..4520
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4670..4690)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4670..4688)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4709..4725)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4709..4725)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4722..4744)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4733..4749
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4757..4763,4764..4781,4782..4787))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4802..4823
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4940..4957)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5111..5699)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5191..5210)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5870..6661,6662..6730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6493..6512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6731..6835)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6861..7316)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6998..7019)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7210..7229
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7387..8371
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagc caccatggac tacaaggacg acgacgacaa gtcccagcct
      121 attatggtac agagaagatc tggacagggt tttcatggaa acagtgaagt aaatgcaata
      181 ctgtctccgc gatcagaaag tggaggcctt ggtgtgagca tggtagaata tgtattaagt
      241 tcttctcctg ctgataaatt ggattctcga tttaggaagg gaaattttgg cactagagat
      301 gctgaaacag atggacctga gaaaggagat caaaaaggca aggcttctcc atttgaggag
      361 gaccaaaaca gagatcttaa acaaggagat gatgatgatt ctaaaataaa tggcagaggt
      421 ttgccaaatg gaatggatgc cgattgcaaa gattttaatc gtactcctgg aagtcgtcaa
      481 gcctctccaa ctgaagtagt tgagcgcttg ggccccaata ctaatccctc agaaggactg
      541 gggcctcttc ctaatcctac agctaataaa ccacttgttg aagaattttc aaatcctgaa
      601 actcagaatc tggatgccat ggaacaagtt ggtctggaat ccttacagtt tgactatcct
      661 ggtaatcagg taccaatgga ctcttcagga gctactgtag gcctttttga ctacaattcc
      721 cagcagcagc tctttcagag gactaatgca ctaacagttc aacagttaac tgcagctcaa
      781 cagcagcaat atgcattagc agcagctcag cagccacata tagctggtgt attctcagca
      841 ggccttgctc cagctgcatt tgtgccaaat ccatacatta ttagtgctgc tcctccaggg
      901 accgatccgt atactgcagc aggattggct gcagcagcta cattagcagg tccagcagtg
      961 gttccacctc agtattacgg cgttccatgg ggggtgtatc cagccaactt atttcagcag
     1021 caagctgcag ctgcggcaaa taacacagcc agtcagcaag cagcatcaca agctcagcct
     1081 ggacagcaac aggttctccg tgctggagca ggtcagcgtc ctcttactcc caatcagggt
     1141 cagcaagggc agcaagcaga atcacttgcg gcagctgcag cagcaaatcc aacattggct
     1201 tttggtcagg gtcttgctac tggcatgcca ggctatcaag tactagctcc aactgcctat
     1261 tatgatcaga ctggtgcctt agtggttggc cctggagcaa ggactggcct tggagctcca
     1321 gttcggttaa tggctccaac acctgtttta attagttcag cagcagcaca agctgcagca
     1381 gcagcagcag ctggaggaac tgcaagtagc cttacaggca gcacaaatgg tctgtttcgg
     1441 ccaattggca ctcagccacc acagcagcag caacagcagc caagcactaa tctgcaatct
     1501 aattcatttt atggaagcag ttctttgact aatagctccc agagtagttc tttattttct
     1561 catggacctg gtcaacctgg aagtacatct cttggctttg gaagtggtaa ctctttgggt
     1621 gctgctatag gctcagccct cagtggattt ggttcatcag ttggcagttc tgcaagtagt
     1681 agtgccacaa ggagagagtc tctatctact agctctgact tgtacaaaag atctagtagc
     1741 agcctagcac ccatagggca accattttac aatagtctgg gattttcctc ctctccaagt
     1801 ccaataggca tgcctctgcc aagccaaact ccaggacatt cacttacgcc accgccatca
     1861 ctttcatcac atggatcctc atccagtttg catttaggag gactgacaaa tggtagtggt
     1921 cgatatatct ctgcagcacc tggagcagaa gcaaaatatc gaagtgcttc aagcacttcc
     1981 agtctattta gctccagcag ccagctcttt cctccttccc ggcttcggta taataggtct
     2041 gatattatgc cttctggccg cagtagatta ttggaagatt tcagaaacaa ccgcttccca
     2101 aaccttcagc ttagagactt gattggacat atagttgagt tttctcaaga ccagcatggt
     2161 tctagattca tacagcaaaa actagagaga gctactccag ctgagcgaca gatggtattt
     2221 aatgaaattc tgcaagcagc ctatcaatta atgactgatg tttttggcaa ctatgttata
     2281 cagaagtttt ttgagtttgg gagtctggat caaaaattag ccctggctac tcgtattcgt
     2341 ggtcatgttc tacccttagc cttgcagatg tatggctgcc gcgttattca gaaagcatta
     2401 gaatctattt cttctgacca gcagagtgaa atggtaaagg agctggatgg tcatgtgctc
     2461 aaatgtgtga aagatcagaa tggaaaccat gttgtacaaa aatgtatcga atgtgttcag
     2521 ccacagtcac tacagttcat cattgatgct ttcaagggac aagtatttgt gctttcaact
     2581 catccttatg gctgcagagt aattcagcgc atcctagagc attgcactgc agaacagacc
     2641 ttacctatct tagaagaact ccaccaacat acagagcagt tggtacagga tcagtatggc
     2701 aattatgtta ttcagcatgt actggaacac ggtcgacctg aagacaagag caaaattgtt
     2761 tccgaaatca ggggaaaggt tttagccctg agtcaacaca aatttgccag caatgtagta
     2821 gaaaagtgtg ttactcatgc ctcccgtgct gagagagctt tactgattga cgaggtttgc
     2881 tgccagaatg atggtcctca cagtgcctta tacaccatga tgaaggacca gtatgccaat
     2941 tacgtggttc aaaagatgat tgatatggct gaacctgctc agagaaagat aatcatgcac
     3001 aagattcgac ctcacattac tactttgcgc aaatacacat acgggaagca tatactggcc
     3061 aagttggaaa agtattattt gaagaatagc ccggacctag gacctattgg aggaccacca
     3121 aatggaatgc tgatgtaccc atacgatgtt ccagattacg ctggcggaca gctggaccaa
     3181 acaccgtccc gacagccaat ccccagggag ggacttcagc tgcatttgcc ccaggtcctc
     3241 gcggatgcgg tctctcgttt agttgtggat aagttcagcg agctgacaga caacttctcg
     3301 tctccacatg cacggcggaa agtcctggca ggagttgtca tgacaaccgg taaggatgtg
     3361 atggacgctc aggtgatttg tgtgacgacg ggaaccaagt gtataaacgg agagtacatg
     3421 agcgatcgag gactggccct taatgactgc catgctgaga tcatcgcacg gcgctcactc
     3481 attagatacc tgtacaatca gctggaatac ttcctcagcg acagcactga agaacatgag
     3541 aaatccatat tcagatggtg cagtgaacat ggctaccggc taaaagatga tattcaattc
     3601 catctgtata tcagcacgtc gccctgtgga gacgcccgga tcttctcccc tcatgaggct
     3661 ggtgccgagg accagggtga ccgacatcca aaccgcaagg cccgtggaca actacgaacc
     3721 aagattgagt ctggagaagg aacgatacct gttcgtacga gtaacaccat tcagacttgg
     3781 gatggagtcc tgcagggaga gcgactgcta accatgtcat gcagtgacaa aattgccaga
     3841 tggaacgtga tcggagtaca gggatctctt ctgagttact tcacagagcc catctatttc
     3901 tccagtttaa ttctgggaag tctgtatcat gctgaccatc tttccagagc catgtaccag
     3961 cgcatcgcag acatggatga tattcccccg ccattcagaa tcaacagacc acttcttagc
     4021 ggtatcagta acacagaagc aaggcaacct gggaaagctc ctaatttcag cgtcaactgg
     4081 acagtaggag accaggggtt ggagatcatt aacgcaacca caggaaaaga tgacctcgga
     4141 agacagtcgc aactttgcaa acacgctctt tacagtcgtt gggtctgtct acatgcaaag
     4201 ctcagtgaga ctctgcggat ccgaggatct cggcctggct cctaccacga ggcaaaacaa
     4261 ggggcggtcg agtatcatga ggcgaaacag actcttttca aagctttcta caaagcggga
     4321 ctgggagcat gggtggaaaa acccatcgaa caagaccagt tttcccttac tccctagcaa
     4381 ggcctctcga gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga
     4441 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     4501 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     4561 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     4621 ttcgcggccg cggcgccaat gcattgggcc cggtacccag cttttgttcc ctttagtgag
     4681 ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     4741 cgctcacaat tccacacaac atacgagccg ggagcataaa gtgtaaagcc tggggtgcct
     4801 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4861 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4921 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4981 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5041 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5101 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5161 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5221 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5281 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     5341 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5401 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     5461 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     5521 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     5581 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     5641 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     5701 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     5761 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     5821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     6361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     6421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     6481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     6541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     6601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     6661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     6721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     6781 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6841 ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     6901 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     6961 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     7021 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     7081 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     7141 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     7201 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     7261 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca
     7321 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     7381 acgccagtcg accgccaatt caatatggcg tatatggact catgccaatt caatatggtg
     7441 gatctggacc tgtgccaatt caatatggcg tatatggact cgtgccaatt caatatggtg
     7501 gatctggacc ccagccaatt caatatggcg gacttggcac catgccaatt caatatggcg
     7561 gacctggcac tgtgccaact ggggaggggt ctacttggca cggtgccaag tttgaggagg
     7621 ggtcttggcc ctgtgccaag tccgccatat tgaattggca tggtgccaat aatggcggcc
     7681 atattggcta tatgccagga tcaatatata ggcaatatcc aatatggccc tatgccaata
     7741 tggctattgg ccaggttcaa tactatgtat tggccctatg ccatatagta ttccatatat
     7801 gggttttcct attgacgtag atagcccctc ccaatgggcg gtcccatata ccatatatgg
     7861 ggcttcctaa taccgcccat agccactccc ccattgacgt caatggtctc tatatatggt
     7921 ctttcctatt gacgtcatat gggcggtcct attgacgtat atggcgcctc ccccattgac
     7981 gtcaattacg gtaaatggcc cgcctggctc aatgcccatt gacgtcaata ggaccaccca
     8041 ccattgacgt caatgggatg gctcattgcc cattcatatc cgttctcacg ccccctattg
     8101 acgtcaatga cggtaaatgg cccacttggc agtacatcaa tatctattaa tagtaacttg
     8161 gcaagtacat tactattgga agtacgccag ggtacattgg cagtactccc attgacgtca
     8221 atggcggtaa atggcccgcg atggctgcca agtacatccc cattgacgtc aatggggagg
     8281 ggcaatgacg caaatgggcg ttccattgac gtaaatgggc ggtaggcgtg cctaatggga
     8341 ggtctatata agcaatgctc gtttagggaa c
//
