LOCUS       sequence-498181-        5287 bp    DNA     circular SYN 26-JAN-2026
DEFINITION  In vitro transcription (SP6 promotor) or mammalian expression (CMV 
            promotor) of the catalytic domain of human Adar2 (E488Q, T375G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5287)
  TITLE     Cifuentes Lab Plasmids 2025
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCS2_hsAdar2_E488Q_T375G
FEATURES             Location/Qualifiers
     source          1..5287
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1339..1473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1363..1382)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1417..1436
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1586..1606)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1586..1604)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1625..1641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1625..1641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1638..1660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1649..1665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1673..1679,1680..1697,1698..1703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1718..1739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1856..1873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2027..2615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2107..2126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2786..3577,3578..3646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3409..3428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3647..3751)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3777..4232)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3914..3935)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4126..4145
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4303..5287
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgccacca tgtacccata cgatgttcca
      121 gattacgctg gaggccagct gcatttaccg caggttttag ctgacgctgt ctcacgcctg
      181 gtcctgggta agtttggtga cctgaccgac aacttctcct cccctcacgc tcgcagaaaa
      241 gtgctggctg gagtcgtcat gacaacaggc acagatgtta aagatgccaa ggtgataagt
      301 gtttctacag gaggcaaatg tattaatggt gaatacatga gtgatcgtgg ccttgcatta
      361 aatgactgcc atgcagaaat aatatctcgg agatccttgc tcagatttct ttatacacaa
      421 cttgagcttt acttaaataa caaagatgat caaaaaagat ccatctttca gaaatcagag
      481 cgaggggggt ttaggctgaa ggagaatgtc cagtttcatc tgtacatcag cacctctccc
      541 tgtggagatg ccagaatctt ctcaccacat gagccaatcc tggaagaacc agcagataga
      601 cacccaaatc gtaaagcaag aggacagcta cggaccaaaa tagagtctgg tcaggggacg
      661 attccagtgc gctccaatgc gagcatccaa acgtgggacg gggtgctgca aggggagcgg
      721 ctgctcacca tgtcctgcag tgacaagatt gcacgctgga acgtggtggg catccaggga
      781 tcactgctca gcattttcgt ggagcccatt tacttctcga gcatcatcct gggcagcctt
      841 taccacgggg accacctttc cagggccatg taccagcgga tctccaacat agaggacctg
      901 ccacctctct acaccctcaa caagcctttg ctcagtggca tcagcaatgc agaagcacgg
      961 cagccaggga aggcccccaa cttcagtgtc aactggacgg taggcgactc cgctattgag
     1021 gtcatcaacg ccacgactgg gaaggatgag ctgggccgcg cgtcccgcct gtgtaagcac
     1081 gcgttgtact gtcgctggat gcgtgtgcac ggcaaggttc cctcccactt actacgctcc
     1141 aagattacca agcccaacgt gtaccatgag tccaagctgg cggcaaagga gtaccaggcc
     1201 gccaaggcgc gtctgttcac agccttcatc aaggcggggc tgggggcctg ggtggagaag
     1261 tccaccgagc aggaccagtt ctcactcacg taacaaggcc tctcgagcct ctagaactat
     1321 agtgagtcgt attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc
     1381 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     1441 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     1501 tttcaggttc agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat
     1561 tgggcccggt acccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     1621 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1681 gagccgggag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1741 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1801 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1861 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1921 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1981 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     2041 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     2101 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     2161 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     2221 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     2281 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     2341 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     2401 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2461 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2521 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2581 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2641 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2701 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2761 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2821 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2881 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2941 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     3001 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     3061 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     3121 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     3181 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     3241 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     3301 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     3361 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3421 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3481 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3541 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3601 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3661 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3721 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     3781 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     3841 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     3901 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     3961 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     4021 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     4081 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     4141 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     4201 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     4261 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagtcgaccg ccaattcaat
     4321 atggcgtata tggactcatg ccaattcaat atggtggatc tggacctgtg ccaattcaat
     4381 atggcgtata tggactcgtg ccaattcaat atggtggatc tggaccccag ccaattcaat
     4441 atggcggact tggcaccatg ccaattcaat atggcggacc tggcactgtg ccaactgggg
     4501 aggggtctac ttggcacggt gccaagtttg aggaggggtc ttggccctgt gccaagtccg
     4561 ccatattgaa ttggcatggt gccaataatg gcggccatat tggctatatg ccaggatcaa
     4621 tatataggca atatccaata tggccctatg ccaatatggc tattggccag gttcaatact
     4681 atgtattggc cctatgccat atagtattcc atatatgggt tttcctattg acgtagatag
     4741 cccctcccaa tgggcggtcc catataccat atatggggct tcctaatacc gcccatagcc
     4801 actcccccat tgacgtcaat ggtctctata tatggtcttt cctattgacg tcatatgggc
     4861 ggtcctattg acgtatatgg cgcctccccc attgacgtca attacggtaa atggcccgcc
     4921 tggctcaatg cccattgacg tcaataggac cacccaccat tgacgtcaat gggatggctc
     4981 attgcccatt catatccgtt ctcacgcccc ctattgacgt caatgacggt aaatggccca
     5041 cttggcagta catcaatatc tattaatagt aacttggcaa gtacattact attggaagta
     5101 cgccagggta cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg
     5161 ctgccaagta catccccatt gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc
     5221 attgacgtaa atgggcggta ggcgtgccta atgggaggtc tatataagca atgctcgttt
     5281 agggaac
//
