LOCUS       sequence-498212-        1759 bp    DNA     linear   UNA 26-FEB-2026
DEFINITION  Mitochondria-tethered construct for the RAPID-Release approach.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ
  TITLE     Evidence for the nuclear import of histones H3.1 and H4 as monomers.
  JOURNAL   EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub
            2018 Sep 3.
  PUBMED    30177573
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-10-1"; volume: "37(19):e98714. doi"; pages: " 
            10.15252/embj.201798714. Epub 2018 Sep 3"
COMMENT     Sequence Label: pCL1010
FEATURES             Location/Qualifiers
     source          1..1759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      57..66
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(474..476,477..479,480..1190)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHWVLK
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(519..540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(780..799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cnnnnnngnn nncntntagc agagctggtt tagtgaccgt cagatccgct agcgccgccg
       61 ccatggccac taaggcagcc cgcaaaagcg ctccggccac cggcggcgtg aaaaagcccc
      121 accgctaccg gccgggcacc gtggctctgc gcgagatccg ccgttatcag aagtccactg
      181 aactgcttat tcgtaaacta cctttccagc gcctggtgcg cgagattgcg caggacttta
      241 aaacagacct gcgtttccag agctccgctg tgatggctct gcaggaggcg tgcgaggcct
      301 acttggtagg gctatttgag gacactaacc tgtgcgccat ccacgccaag cgcgtcacta
      361 tcatgcccaa ggacatccag ctcgcccgcc gcatccgcgg agagagagct ggaggtggct
      421 caggaggtgg ctcaggaggt ggctcaggtg gaggcgcgct accggtcgcc accatggtga
      481 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
      541 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
      601 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
      661 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
      721 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
      781 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
      841 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
      901 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      961 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     1021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     1081 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcactgg gtccttaagg
     1141 agttcgtgac cgccgccggg atcactctcg gcatggacga actgtacaag tactcagatc
     1201 tcgagggaag ttccggcggg aacggaagaa aaactgtaaa attccaaagc ggtggatccg
     1261 aaaccgtgcc ntttccaaac ggtggccggg ggaaacggng gattcagtgg gcccggaaag
     1321 tggnncngna agtaaccccn ncntggccnt tcttggnnaa aaaattggcn ttaaagcctg
     1381 gaaaaaacca accccnnnnn tttnggcaaa cacnnnnnaa aggcnnnttt aaagtnggac
     1441 accttnnnnc caantannna ncggnnccca accctgaaaa acnntttnan ccnnnttgnc
     1501 nnnnnnnngg nggccnggaa gggcgntann nnaaanngnn nnntgaatat aannccnngg
     1561 ggnnnantta atnnttccnc ccngnnccng nnnnnnagaa caganctgag ggaattgnan
     1621 nggnnnnnnn gaaaatctnn nntnnnnnnn nnaacttggn naatgggnnn nnnnacnnnn
     1681 ngncgtnngn ngttatcnga nggatcnnnt annnnttnnn aattnaacat ttnnnnnctn
     1741 aaannntnnn ncaannnga
//
