LOCUS       sequence-498215-        1278 bp    DNA     linear   UNA 27-JAN-2026
DEFINITION  ASF1A tagged on the N-terminus with mCherry and a Nuclear Export 
            Sequence to make it cytoplasmic..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Apta-Smith MJ, Hernandez-Fernaud JR, Bowman AJ
  TITLE     Evidence for the nuclear import of histones H3.1 and H4 as monomers.
  JOURNAL   EMBO J. 2018 Oct 1;37(19):e98714. doi: 10.15252/embj.201798714. Epub
            2018 Sep 3.
  PUBMED    30177573
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 27, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2018-10-1"; volume: "37(19):e98714. doi"; pages: " 
            10.15252/embj.201798714. Epub 2018 Sep 3"
COMMENT     Sequence Label: pCL1013
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             73..99
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             151..171
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gnnnnncnnc nnggcggcat ggacgagctg tacaagtccg gactcagatc tcgagctccc
       61 gtcccacttc agttgcctcc cttagagcgt ttaactctag actgcaacga gcaagcttcg
      121 aattctgcag tcgacggtac cggcagcggc gaaaaccttt acttccaggg ccatatggcg
      181 ggatttggtg ctatggagaa atttttggta gaatataaga gtgcagtgga gaagaaactg
      241 gcagagtaca aatgtaacac caacacagca attgaactaa aattagttcg ttttcctgaa
      301 gatcttgaaa atgacattag aactgtcttt cctgagtata cccatcaact ctttggggat
      361 gatgaaactg cttttggtta caagggtcta aagatcctgt tatactatat tgctggtagc
      421 ctgtcaacaa tgttccgtgt tgaatatgca tctaaagttg atgagaactt tgactgtgta
      481 gaggcagatg atgttgaggg caaaattaga caaatcattc cacctggatt ttgcacaaac
      541 acgaatgatt tcctttcttt actggaaaag gaagttgatt tcaagccatt cggaacctta
      601 cttcatacct actcagttct cagtccaaca ggaggagaaa actttacctt tcagatatat
      661 aaggctgaca tgacatgtag aggctttcga gaatatcatg aaaggcttca gacctttttg
      721 atgtggttta ttgaaactgc tagctttatt gacgtggatg atgaaagatg gcactacttt
      781 ctagtatttg agaagtataa taaggatgga gctacgctct ttgcgaccgt aggctacatg
      841 acagtctata attactatgt gtacccagac aaaacccggc cacgtgtaag tcagatgctg
      901 attttgactc catttcaagg tcaaggccat ggtgctcaac ttcttgaaac agttcataga
      961 tactacactg aatttcctac agttcttgat attacagcgg aagatccatc caaaagctat
     1021 gtgaaattac gagactttgt gcttgtgaag ctttgtcaag atttgccntg tttttcccgg
     1081 gaaaaattaa tgcaaggatt caatgaagnt atgggcaata gaggcacaac annaagttca
     1141 aaataaataa gcaacacgct agaaagggtt natgaaaatt cttccaacta ctgggtaact
     1201 ggacatggag tggatgccga aacaataccg aaagcctccc gacctgggnn nttttaaaga
     1261 aaaactaatt tagcctca
//
