LOCUS       sequence-498228-        5366 bp    DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Hanish JP, Yanowitz JL, de Lange T
  TITLE     Stringent sequence requirements for the formation of human 
            telomeres.
  JOURNAL   Proc Natl Acad Sci U S A. 1994 Sep 13. 91(19):8861-5.
  PUBMED    8090736
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1994 Sep 13. 91(19):8861-5."
COMMENT     Sequence Label: pSXneo 270(T2AG3)
FEATURES             Location/Qualifiers
     source          1..5366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2111..2914
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2174..2193)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2784..2803
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2921..2969
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(3020..3039)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3021..3039)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3126..3143)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3297..3885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3377..3396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4056..4847,4848..4916))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4679..4698
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4917..5021)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5089..5107
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5145..5167)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5267..5286
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttaggtga cactatagaa ccagatctat cgatgcggcc gcaattccga accctaaccc
       61 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      121 taaccctaac cctaacccta accctaaccc taaccctaac cctaacccta accctaaccc
      181 taaccctaac cctaacccta accctaaccc taaccccaac cccaacccca accccaaccc
      241 taaccctaac cctaacccca accccaaccc caaccccaac cccaacccca accctaaccc
      301 caaccccaac cccaacccca accccaaccc caaccctaac cctaacccca accccaaccc
      361 caaccccaac cctaacccta accctaaccc caaccccaac cctaacccta accctaaccc
      421 taaccctaac cctaacccta accccaaccc taaccctaac cctaacccta accccaaccc
      481 caaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
      541 caaccccaac cctaacccca accctaaccc taaccctaac cctaacccta accctaaccc
      601 taaccccaac cccaacccca accccaaccc caaccccaac cccaacccca accccaaccc
      661 taaccctaac cctaacccta accccaaccc caaccctaac cctaacccta accctaaccc
      721 taaccctaac cctaacccca accccaaccc caaccccaac cccaacccca accccaaccc
      781 caaccctaac cctaacccta accctaaccc taaccctaac cccaacccta accctaaccc
      841 taacgctaac cctaacccca accctaaccc caaccccaac cccaacccca accccaaccg
      901 gaattccgcg gaattcgagc tcggtacccg gccgcaattc cgaaccctaa ccctaaccct
      961 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
     1021 aaccctaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
     1081 aaccctaacc ctaaccctaa ccccaacccc aaccccaacc ccaaccctaa ccctaaccct
     1141 aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ctaaccccaa ccccaacccc
     1201 aaccccaacc ccaaccccaa ccctaaccct aaccccaacc ccaaccccaa ccccaaccct
     1261 aaccctaacc ctaaccccaa ccccaaccct aaccctaacc ctaaccctaa ccctaaccct
     1321 aaccctaacc ccaaccctaa ccctaaccct aaccctaacc ccaaccccaa ccccaacccc
     1381 aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccccaaccct
     1441 aaccccaacc ctaaccctaa ccctaaccct aaccctaacc ctaaccctaa ccccaacccc
     1501 aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccctaa ccctaaccct
     1561 aaccctaacc ccaaccccaa ccctaaccct aaccctaacc ctaaccctaa ccctaaccct
     1621 aaccccaacc ccaaccccaa ccccaacccc aaccccaacc ccaaccccaa ccctaaccct
     1681 aaccctaacc ctaaccctaa ccctaacccc aaccctaacc ctaaccctaa cgctaaccct
     1741 aaccccaacc ctaaccccaa ccccaacccc aaccccaacc ccaaccggaa ttccgcggaa
     1801 ttcgagctcg gtacccgggg atcctctaga gtcgagcagt gtggttttca agaggaagca
     1861 aaaagcctct ccacccaggc ctggaatgtt tccacccaat gtcgagcagt gtggttttgc
     1921 aagaggaagc aaaaagcctc tccacccagg cctggaatgt ttccacccaa tgtcgagcaa
     1981 accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc ggggcggcgc
     2041 ggtcccaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc
     2101 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc
     2161 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     2221 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     2281 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     2341 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     2401 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     2461 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     2521 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     2581 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     2641 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     2701 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     2761 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     2821 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     2881 cgccttctat cgccttcttg acgagttctt ctgaggggat cggcaataaa aagacagaat
     2941 aaaacgcacg ggtgttgggt cgtttgttcg gatccgtcga gcagctgaag cttgcatgcc
     3001 tgcaggtcga ggccggtctc cctatagtga gtcgtattaa tttcgataag ccaggttaac
     3061 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3121 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3181 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3721 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3961 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4021 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4081 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4141 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4201 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4261 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4321 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4381 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4441 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4501 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4561 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4621 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4681 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4741 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4801 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4861 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4921 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4981 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5041 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5101 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5161 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5221 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5281 attgtactga gagtgcacca tatggacata ttgtcgttag aacgcggcta caattaatac
     5341 ataaccttat gtatcataca catacg
//
