LOCUS       sequence-49826-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kashatus DF, Lim KH, Brady DC, Pershing NL, Cox AD, Counter CM
  TITLE     RALA and RALBP1 regulate mitochondrial fission at mitosis.
  JOURNAL   Nat Cell Biol. 2011 Aug 7;13(9):1108-15. doi: 10.1038/ncb2310.
  PUBMED    21822277
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Cell Biol"; date: "2011-08-7- 7"; volume: "13"; issue: "9"; 
            pages: "1108-15"
COMMENT     Sequence Label: pGEX-5X2-hDrp1(518-736)-Flag
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..65)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(630..641)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(671..693)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ggcagatcgt cagtcaggtc acgatgcggc cgctcgagtc acttgtcgtc atcgtctttg
       61 tagtcccaaa gatgagtctc ccggatttca gcaataattt gactggctcc ttgtaatgcc
      121 tttagcatat cagctgcttc tttcctgcgc tgtgccatgt cctcagattc tgtcagaaga
      181 tcatccaata aggatgattt atacagctgg cctactagct cactctgaag agtgtctttc
      241 acatgattaa acaaaaaatg cattactgcc tttggcacac tgtcttgaat attctttctg
      301 acaatgagga aatatgattt aatgagtcgt tcaataacct cacaatctcg ctgttcccga
      361 gcagatagtt ttcgtgcaac aggaactggc acatctagca ggttcacggc atgacctttt
      421 tgtggactgg ctggcataat tggaatgggt tttgattttt cttctgctaa taactcttca
      481 gcttttgaag ttttcagcat tcctctccag ttgcctgtgg ttggttcttg aacaccatct
      541 ccaaccccac cacctccaga tgcaaccttg tctcgtgata cagctgaagg taattctctg
      601 gctagcctgt ttcgaattcc ggggatccca cgaccttcga tcagatccga ttttggagga
      661 tggtcgccac caccaaacgt ggcttgccag ccctgcaaag gccatgctat atacttgctg
      721 gatttcaagt acttatcaat ttgtgggata gcttcaatac gttttttaaa acaaactaat
      781 tttgggaacg catccaggca cattgggtcc atgtataaaa caacatcaag agcgtcatac
      841 aacatgaagt caggatgggt tac
//
