LOCUS       sequence-498270-        9600 bp    DNA     circular SYN 26-JAN-2026
DEFINITION  Contains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola 
            (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in 
            Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Cunningham FX Jr, Gantt E
  TITLE     A study in scarlet: enzymes of ketocarotenoid biosynthesis in the 
            flowers of Adonis aestivalis.
  JOURNAL   Plant J. 2005 Feb;41(3):478-92.
  PUBMED    15659105
REFERENCE   2  (bases 1 to 9600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2005-02"; volume: "41(3)"; pages: "478-92"
COMMENT     Sequence Label: pAC-BETAipi
FEATURES             Location/Qualifiers
     source          1..9600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..546
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     447..466
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     663..680
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        1072..1174
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1175..1834
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1241..1260)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2043..2062
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        complement(join(9461..9466,9467..9483,9484..9489))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
       61 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
      121 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
      181 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
      241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
      301 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
      361 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
      421 ggagagcgca cgagggagcc gccaggggga aacgcctggt atctttatag tcctgtcggg
      481 tttcgccacc actgatttga gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta
      541 tggaaaaacg gctttgccgc ggccctctca cttccctgtt aagtatcttc ctggcatctt
      601 ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc
      661 gtagcgagtc agtgagcgag gaagcggaat atatcctgta tcacatattc tgctgacgca
      721 ccggtgcagc cttttttctc ctgccacatg aagcacttca ctgacaccct catcagtgcc
      781 aacatagtaa gccagtatac actccgctag cgctgatgtc cggcggtgct tttgccgtta
      841 cgcaccaccc cgtcagtagc tgaacaggag ggacagctga tagaaacaga agccactgga
      901 gcacctcaaa aacaccatca tacactaaat cagtaagttg gcagcatcac ccgacgcact
      961 ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt
     1021 ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca
     1081 cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg tattttttga
     1141 gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1201 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1261 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     1321 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     1381 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     1441 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     1501 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     1561 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1621 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1681 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1741 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1801 acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc
     1861 ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc
     1921 gaaagcaaat tcgaccccaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     1981 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     2041 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     2101 acgatgcgtc cggcgtagag gatctaccac ctgcgtgcgc tgggtgttga tgtgcgcctg
     2161 acccgctccg acgtgacaaa gggccacccg gagtactatc aggactacgc ccgccgcctg
     2221 gcagaggaga cgccgggtgc ttactatatt gatcagttct ctaacccggc caacccgctg
     2281 gcgcacgcca ccaccaccgc accggagctg ttcgaccagc tggcaggcag ggttgatgcc
     2341 gtggtagtag gcgttggctc cggcggcacg ctgggtggct tgcaggcatg gtttgccaaa
     2401 cactctccgc ataccgaatt cgtgctggcc gatcccgttg gctcgattct ggcggatcag
     2461 gttgagaccg gacagcacgg cgaggcggga tcccgcgccg gggctacctg aagcggctct
     2521 acgtcgccca ccgcctgcac cacgcggtgc gcggccggga gggctgcgtc tccttcggtt
     2581 ttatttacgc ccgcaagcct gccgacctac aggcgatcct gcgtgaacgt catggccgcc
     2641 cgcctaaacg ggacgctgcc aaagaccggc cggacgcggc gtcaccctcg tcgtcttcgc
     2701 ccgaataacc tgccccggtg ccgccatcag catggcaatt ttttcacctt tgctggtgtg
     2761 ctggcggcga tcccaggcgc tgcctcccgc cgcttttacc ttaataccga tctcccggta
     2821 gacgctgcgg gcggtggcga tcgcccaggc gcagcgcggc ggcagatcgt gtagcccggc
     2881 ctgggaggag atgtagtacg gctctgcggc atcaataagc cgctccgcca cccgcgccag
     2941 cgcggcccga ttctcccgcg cggcatagtt ctccggggtc agcccggcat cctgcagcca
     3001 ctcggcgggc agatagcagc ggtcaatagc cgcatcgtca ataatatccc gggcgatatt
     3061 cgtcagctgg aaggccagcc ccagatcgca ggcgcgatcc agcacccgct catcccgcac
     3121 gcccatcacc ctggccatca tcagacccac cacgcccgcc acgtgatagc agtagcgcag
     3181 cgtatcctca aaggtgacat agcgggtctg agccacgtcc atcgcaaagc cgtcgaggtg
     3241 atcgagcgcc atgcggggcg taataccgtg ggtcagcgcc acctcctgaa aggcagcgaa
     3301 ggccggatcc tgcatctcgg ccccttcaaa cgccgccagg gtcagcgtgc gcagccgggc
     3361 caggcgctgg gtggcctcct cctccgccgc ggcctcgctg gcgaagccgt gggtctggtc
     3421 gtcaatgacg tcatcgcagt ggcggcacca ggtgtagagc atcagcacgc tacggcgggt
     3481 ggccgggtcg aacagcttcg cagcggtggc aaaacttttc gagccgttgg ccatggtctg
     3541 cgtggcgtgg tcaagcagcg gcggttggct cattgcagat cctcaatcat caggctggcg
     3601 gtggctttcg ccgaggccac tacgccagga atgcccgccc cagggtgagt acctgcgccc
     3661 accaggtaga ggttggcaat gtcgctgtcg cggttgtgcg ggcggaacca ggcgctttgg
     3721 gtcagcagcg gctcgatgga gaaggccgat cccagatgcg catccagcgt gtcgtggaag
     3781 tctgccgggg taaagatccg ctgggtcacc agctggctac gcaggccggg catatagcgc
     3841 tcttcaaggt agtcaaagat gcggtcgcgc agcttcggcc cctcctgcgc ccagtccagc
     3901 ggcgcgttgc caagatgcgg caccggggcc agcacgtaga agctggcgca gccgggaggc
     3961 gcgagcgagg gatcggtcac gcagggcgag tgcaggtaga gcgagaagtc atccgccagc
     4021 gcgctgccgg taaagatctc gtcgatcagc tcccggtagc ggggaccaaa acagatggta
     4081 tggtgcgcca gctgggaatg aggctggttc aggccgaagt agagcacaaa cagcgagttg
     4141 ctcatgctct tgcgctccag cgctgccgcc cgcttctgcc ccaccggatg gtggccgagc
     4201 agctttttat aggtgttcac cacgtcagcg ttcgaggcta cggcgtcggt gtcaaagatc
     4261 cgaccatccg ccagccggac ctggcttacg cggttatcgg ccaccaccag ctcttcgacc
     4321 cgggcgttga gttcgatctc cccgcccaga tcggtaaaca gcttcaccat gccgttcacc
     4381 agcgccccgg tgccgccctc agggaaccag accccccact cccgctcaag ggcgtggatc
     4441 agggtgtaga tggacgaggt ggtgaagggg ttgccgccta ccagcaggga gtggaacgag
     4501 aaggcctgcc gcagatgctc atcctcaata aagcgcgaaa ccgactggta gacgctctgc
     4561 cacgcctgga gcttaagcag ctgcggcccg gcgcgcagca tgtcgcgaaa agagaggaac
     4621 ggcacgctgc cgaggcgcaa atatccctcc tggaataccg cctgggagta agccagaaag
     4681 cgccggtagc cctcgacgtc gcgggggttg aactgggtaa tctgcgcctc aagctcggcg
     4741 ctgtcgttag catagtcgag ggtcttcccg gactcccagc agagtcggta gaagggtttt
     4801 accggcagca gcctgacgta atcctccatg cgcctgccgg ccagggtgaa cagcgcctca
     4861 agcgcggtag gatcggtgat caccgtcggc ccggcgtcaa aggtaaagcc ctggtcatgc
     4921 cagacgtagg cccgaccgcc gggcttgtcc cgctgctcca gcagtacggt tgggatccct
     4981 gccgcctgca ggcgaatcgc cagcgccagg ccaccaaagc ctgcgccaat cacaacggtt
     5041 tttttcatcc tttatctcgt ctgtcaggaa aatggttcag cgccgcccgc caggcttcgc
     5101 ccagcggaac cggtggcttg cccgtcaaaa tgcgggcctt atcaaagaga gagagccgac
     5161 cggcgtaaaa gcgctctacg gtgggctccg gcagcccata aaagcgctgc atcacccgcc
     5221 agcggttctc ctcgcgcccg gccaggaaaa gcatccggtt cagcaggcgg aagaatccct
     5281 gcctgcgcca gtggcgttcg gcaaactgcc gggtgagctg atagagcgga acgctgccca
     5341 gccgcgggct gtcggcaatc gcgtcggcaa gggccaccgc cagcggcagc gaatagccag
     5401 tggtagggtg aaatagccca gcccgcattc ccgagcgcgg cacgcccggc gcatcggccc
     5461 acagagcctg gatgtcaccc gccagggtaa tcggcagaca gccggtctcc tcgcgttcaa
     5521 gctgggccag ctgccaccct ttgctgtgag catagtcggt aaccgtctgg cgtagggcat
     5581 tatcatcacg ctgcgggaca ttggcgtagc gcgtatcctc gatcagcagc gtgtcggcgg
     5641 agagcggcag cgtgtagaca aagcgatagc cctgctgctg cgccaccgtg gcatccatca
     5701 ggatcggtac ggtcaggccg tggggctgtg tcagccgcca ctgctgacca agaaagagct
     5761 gatagccggt ttgcatcgcc gaactggcgg tcacgccgcg tccgtcaatc accgctccgg
     5821 caagcagcgc ctcaccgttg gcaaggcgca cgctattggg taacacctcg ctcaccgaac
     5881 agtttagcca gatgttctcc cccagcgcct gatgcagggc ctcggcaaag cgctctgagg
     5941 taatggagta gtagccgcgc gcgaggcgac ggcgaagatc gggaaactgc acctcatagc
     6001 ccggccaggc gtgggccacc agcggggcca gccaggcgtg ctgcccggga gtcagatcgt
     6061 cttcatggaa tgaccaggta tggttcccgc cgggctgctc cccggcctcg atcagcagca
     6121 ggttaagctg cgggtagcgc tggcgcagac gccaggcgat cagcccgttg gccaggccgc
     6181 cgccgactaa aatcagatcc ctcacgatac gctctcactc cctgctatgg cctgttcaat
     6241 cagggtcgcc gcacggggcg tgcccccggc gtcgttaagc tgctggcggg ccgtcgccag
     6301 acgctgatgc agcgtctcat cccccagcag ttgggcaatc tcatccgcca gcgtctgctg
     6361 tctggcaaag cgcgataccc ggcgacccag cccgttatag accagccggg cagccacggc
     6421 gggctggtcg aaagagagtg gcaccgccag caccggcgtc gcggcagcca gcgcatccag
     6481 tacggtattg agaccgccgt gggtgatcac cagattagcc tcggcaacgt agcgcggctg
     6541 gtcgagctta tcgatgataa gctgtcaaac atgagaatta caacttatat cgtatggggc
     6601 tgacttcagg tgctacattt gaagagataa attgcactga aatctagaaa tattttatct
     6661 gattaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa
     6721 acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga
     6781 accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt
     6841 aaccggcgca tgacttcaag actaactcct ctaaatcaat taccggtggc cgcgttgggc
     6901 catctccagc gccagggcat gcagcgccac cgcatgactg tagagcggcg gtgccacaat
     6961 ggcaaaatgg ctcatggcgt atcccgcaca tagcggatat cagcctgctt tagatcgctc
     7021 aggctgcggc tgccggtgca gaagcaggcc acccgcagct gctcaataat cacgttgaag
     7081 tgatcgatca ccttctccgt ggagacgcct gcgctgccga gcacggcggc ggcctgccct
     7141 accatgcacg cgccgagccg cagcgctttc gccgcgtcga cgccgttttt aatcccgccc
     7201 gaggcaataa ggggcatctg cggccaggcc tcggcaatgt caaccagcgc ctcagcggtg
     7261 gggatccccc agtcggcaaa gacgttggcc acgctgcgct gctgctcggt ggccgcccgc
     7321 tcgccttcaa cggcggccca gctggtgccg cccgcgcccg cgacgtcaat cacggtaacg
     7381 ccggcatcga tcagctgccc ggccacggtt cgggagatac cggctcccac ctctttcacc
     7441 accagcggaa cgggcagctc gcggaccaga gtttcaatag ccgccagccg tccgcgccag
     7501 tcgcgatcgc cgccgggctg tagcgcctcc tgcagcgggt ttaggtgcac aatcagcgca
     7561 tccgcctcga tcatctccac ggcccgtcgg gcgtaatcaa taccttttct gccggtcagc
     7621 tgcgccgcgc cgaggttcgc cagcagcggc acgtccggag ccagctgccg cagggtttta
     7681 tccagcccta agcccgcgtc gctctcaatg gcgacgcgct gggagcccac ccccatcgca
     7741 atttttagca cctgcgccgc ctcggcgagg tggcggttga tatggcgcga gcgctcaacg
     7801 ccgccggtca tggagctgat cagcagcgga gcctgtagct gccgattcag gaaggtggtt
     7861 tccagcgtga tgtcgctaaa attcagctct ggcagggcgc agtgggtaaa gcgccagcgc
     7921 tcaaaacctg cgctagcctg agttacggcg cgacgggggt cgagaacgat atccagatga
     7981 tcgttcttac gctgaacaag gcgctcgtcc ttcattcatc actactcctg taatgtggtt
     8041 ccatcgttat gtactgcttg aacccaaaag ggcggtatca ggcgattttc atgaccggtg
     8101 accagtcggc aaggtgatgg ccaaaccaca gatgcataaa ctgtcggatg gcgccgccct
     8161 gcggacaggc aaaagtgagg tgtttgtcgg cggaatcaat atgctcgcgc agcttttgcc
     8221 gggccgcgtc tgcgcccagc cggttgacca gcgtcgattt tcccgcgtcc ttattgcgat
     8281 ctttaccggt ttccgggtga tcgtcacgca gatcgtccag cagttgaaac gcctggccga
     8341 agtcgagggc gaaggcgtgc agcgtctctc gcgtgctcgg cgacgaggcg gaagcaatgg
     8401 cgacgatctg cagcatcgcg ctgaacagaa tgccggtctt gaggtggttg gtgctgagga
     8461 tagcgtcagg ggtacggtcg agggcggcat cgttaagatc gcgaaactgc cccagtacca
     8521 ggccctgcac gcccacggcg gtagagagct cgttgaccgc ctgggcacgc ctctcccccg
     8581 gcagatcgcc ggtggcggcg atcagaccaa aggctttaga gagcagccca acggaggcaa
     8641 ggatcgccac gctctcacca aattttttgt gggtagtggg ctgaccgcgg cgcagctcgg
     8701 cgttgtccat gcagggcatg tcgtcgagca tcagcgacgc ggtatgggtc agttcaacgg
     8761 cgcaggcgag atcgagcagc gtaggcatac tgccctggta gcggaggtcg cgggcggcca
     8821 gcagcatcag cagcggacgg atccgtttac cgggtgccat gacgccttca cgcatcgcaa
     8881 ggctgacgat atcctggctg tcggtttcag gtaacaggcc agccaggtga tcgtcaatgg
     8941 attgtctcat tacttctatt tcgcgatgag gcgaaacgcc cgctttactg ccactcacca
     9001 ttgcaaggtt acccgctgaa ttgtctttta gtatcagtta accgtagacc gcgcggatag
     9061 ctctcgccag gttctgaagc agttttacat tgtgcgaagc atgagacgct gtgcctttag
     9121 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa
     9181 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat
     9241 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg
     9301 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca
     9361 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt
     9421 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata
     9481 agctgtcaaa catgagaatt acaacttata tcgtatgggg ctgacttcag gtgctacatt
     9541 tgaagagata aattgcactg aaatctagaa atattttatc tgattaataa gatgatcttc
//
