LOCUS       sequence-498377-        2577 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2577)
  TITLE     Plant Golden-gate N-Terminal and C-Terminal Tags.
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pICSL30055
FEATURES             Location/Qualifiers
     source          1..2577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(57..77)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(84..104)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(420..437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(591..1179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(671..690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1350..2141)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2363..2467)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2535..2553
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgtggtctc acattgatcc tccgccacca gaaccaccac cacctgatcc accacccacc
       61 ttacgctttt tctttgggtc ctcaaccttc cgcttcttct taggaccacc gtactcatca
      121 atacccagag cgtcggtgaa catctgctcg aactcaaaat cagccatatc aagagcgccg
      181 tatggagcag agtcatgagg agtgaaacca ggaccaggag aatcaccatc accgagcata
      241 tccaggtcga agtcatccag agcatcagca tgagccatag caacatcctc accatccagg
      301 tgcagctcat caccaagaga aacatcagta ggaggagcca tggtgagacc acagagtgat
      361 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      421 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      481 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      541 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      601 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      661 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      721 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      781 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      841 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      901 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      961 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1021 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1081 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1141 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1201 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1261 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1321 agtatatatg agtaaacttg gtctgacagt tatttgccga ctaccttggt gatctcgcct
     1381 tttacgtaat ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
     1441 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
     1501 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
     1561 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
     1621 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
     1681 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     1741 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
     1801 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     1861 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
     1921 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     1981 accgtaacca gcaaatcaat atcgctgtgt ggcttcaggc cgccatccac tgcggagccg
     2041 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     2101 gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa ctttgtttta
     2161 gggcgactgc cctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg
     2221 gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtaccccaaa aaaaaacagt
     2281 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     2341 tggaccagtt gcgtgagcgc atactcttcc tttttcaata ttattgaagc atttatcagg
     2401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2461 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2521 cattaaccta taaaaatagg cgtatcacga ggccctttcg tccactgaag agccact
//
