LOCUS       sequence-498480-        8737 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Lentiviral vector encoding a truncated promoter region of human 
            SNAIL gene driving a luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8737)
  AUTHORS   Chhe K, Kalyanaraman B, Spielberger SA, Lin HH, Taylor SR, Farkas ME
  TITLE     Reporter-Mediated Evaluation of the Circadian Oscillations of SNAIL 
            Across In Vitro Models.
  JOURNAL   Clocks Sleep. 2025 Sep 28;7(4):54. doi: 10.3390/clockssleep7040054.
  PUBMED    41133664
REFERENCE   2  (bases 1 to 8737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Clocks 
            Sleep."; date: "2025-09-28"; volume: "7(4):54. doi"; pages: " 
            10.3390/clockssleep7040054"
COMMENT     Sequence Label: SNAIL-Luc
FEATURES             Location/Qualifiers
     source          1..8737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(51..70)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        246..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        631..829
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             847..1027
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1074..1199
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1696..1929
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2114..2158
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2452..2569
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(2668..2684)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2669..2685)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        2684..2998
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2692..2710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2728..2746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2763..2781
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2799..2817
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2835..2853
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2870..2888
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2906..2924
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2971..2995
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3456..3465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3462..5114
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3528..3546)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     primer_bind     5116..5132
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5116..5132
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5181..5201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5186..5515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5366..5501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5529..6128
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5529..5548)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6025..6045
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6743..6923
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(6985..7573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7065..7084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7744..8535,8536..8604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8367..8386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8605..8709)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgatcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
       61 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      121 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      181 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
      241 gcgttgacat tgattattga ctagctagtt attaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
      841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     1021 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     1081 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     1141 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     1201 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1261 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1321 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1381 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1441 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1501 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
     1561 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     1621 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     1681 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     1741 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     1801 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     1861 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     1921 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     1981 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     2041 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     2101 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     2161 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     2221 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     2281 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     2341 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
     2401 cggatcggca ctgcgtgcgc caattcgaca aatggcagta ttcatccaca attttaaaag
     2461 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2521 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2581 cagggacagc agagatccag tttggttagt accgggccgg cccgctctag ctagacgtat
     2641 taccgccaga tcccccatca agcttatcga taccgtcgac ctcgagttta ctccctatca
     2701 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt
     2761 actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg
     2821 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt
     2881 gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag
     2941 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     3001 gagaattcag gtgacccgcc tcttaacggt cgccgcgtcc cgtctctccc ccaccaaagc
     3061 acacttccct ttgcattgta attatctgtt tacttcgtct gtctccctca ctggaccaga
     3121 agctaccctt cgggagaggc tctgagtgtt ctgtccgggg ctgtgccctg gccccaggta
     3181 cagtgcccca cacgtgctgg gcgctccgta aacactggat aagggaagga acgggtgctc
     3241 ttggctagct gggccaggct gctttgcaaa aaggccgtgg catttcaagt cgccgagagc
     3301 cacgtgcggt gtccctttcc tcgcttcctc cccagcgatg tgcgtttccc tcgtcaatgc
     3361 caagcttctc gaggggcggg gccttatctg ccccgcccgc ggccgctcga gatctgcgat
     3421 ctaagtaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca
     3481 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
     3541 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     3601 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
     3661 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     3721 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     3781 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     3841 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     3901 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     3961 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     4021 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     4081 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     4141 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     4201 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     4261 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     4321 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     4381 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     4441 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     4501 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     4561 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     4621 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     4681 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     4741 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     4801 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     4861 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     4921 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     4981 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     5041 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     5101 agatcgccgt gtaattctag aactagtgga tcccccgggc tgcaggaatt gatggccgcc
     5161 accgcggtgg aggaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5221 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5341 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5401 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5461 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     5521 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
     5581 gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg
     5641 atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg
     5701 ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca
     5761 cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt
     5821 tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc
     5881 ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg
     5941 gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg
     6001 ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg
     6061 tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg
     6121 gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca cgaccccatg
     6181 catcgatcaa ttcgatgggg gatcaattcg ataagtcgag gtcgacgacc tcgatcgaga
     6241 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6301 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6361 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6421 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6481 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6541 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6601 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6661 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6721 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6901 gtcagtgtgg aaaatctcta gcagcatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6961 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7021 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7081 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7141 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7201 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7261 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7321 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7381 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7441 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7501 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7561 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7621 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8701 ggttccgcgc acatttcccc gaaaagtgcc acctgac
//
