LOCUS       sequence-498488-        7714 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and 
            EGFP tagged human Sec 61B in mammalian cell lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Shen Z, Gelashvili Z, Niethammer P
  TITLE     Endoplasmic reticulum disruption stimulates nuclear membrane 
            mechanotransduction.
  JOURNAL   Nat Cell Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9.
  PUBMED    41366519
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2025 Dec 9. doi: 10.1038/s41556-025-01820-9."
COMMENT     Sequence Label: PSB-CMV-Puro
            ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGF...
FEATURES             Location/Qualifiers
     source          1..7714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        10..389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..593
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     544..564
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        638..656
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      676..685
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1561..1581
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1585..1605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1609..1629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1645..1701
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(1708..1710,1711..1713,1714..2424)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1708..1710,1711..1713,1714..2424)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1753..1774)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2014..2033)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2361..2382
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2431..2721
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     primer_bind     complement(2753..2770)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2759..2983
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        2990..3347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2990..3181
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3013..3033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3198..3333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3375..3974
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3375..3394)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3871..3891
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3988..4007)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    4017..4098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     4042..4061
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4573..4593)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4573..4591)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4612..4628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4612..4628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4625..4647)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4636..4652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4660..4666,4667..4684,4685..4690))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4705..4726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4843..4860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5014..5602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5094..5113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5773..6564,6565..6633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6396..6415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6634..6738)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6765..7220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6852..6871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7062..7083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7346..7368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7360..7377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7361..7377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7387..7405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
       61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
      601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
      661 cccaagctgg ctagcgccac catggatgat tttctgaaca gcgcgatgag cagcctgtat
      721 agcggctgga gcagctttac caccggcgcg agcaaatttg cgagcgcggc gaaagaaggc
      781 gcgaccaaat ttggcagcgg accggtcatg gtgtctaagg gcgaagagct gattaaggag
      841 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
      901 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggccgtcgag
      961 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaaa
     1021 accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt ccctgagggc
     1081 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     1141 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1201 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac cgagaccctg
     1261 taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct cgtgggcggg
     1321 ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc taagaacctc
     1381 aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga ggccgacaaa
     1441 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1501 ctggggcaca aactgaccgg actcaggtcc cgagctcaag cttcgaattc ccgggtcgat
     1561 ccaaaaaaga agagaaaggt agatccaaaa aagaagagaa aggtagatcc aaaaaagaag
     1621 agaaaggtat ccggtggaag cggagctact aacttcagcc tgctgaagca ggctggagac
     1681 gtggaggaga accctggacc tagatccatg gtgagcaagg gcgaggagct gttcaccggg
     1741 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     1801 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1861 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     1921 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1981 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2041 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2101 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     2161 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     2221 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     2281 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     2341 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     2401 ctcggcatgg acgagctgta caagagatct atgcctggtc cgacccccag tggcactaac
     2461 gtgggatcct cagggcgctc tcccagcaaa gcagtggccg cccgggcggc gggatccact
     2521 gtccggcaga ggaaaaatgc cagctgtggg acaaggagtg caggccgcac aacctcggca
     2581 ggcaccgggg ggatgtggcg attctacaca gaagattcac ctgggctcaa agttggccct
     2641 gttccagtat tggttatgag tcttctgttc atcgcttctg tatttatgtt gcacatttgg
     2701 ggcaagtaca ctcgttcgta atctagaggg cccgtttaaa cccgctgatc agcctcgact
     2761 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     2821 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     2881 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     2941 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggaccggac tgaggcggaa
     3001 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     3061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     3301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctagattttc
     3361 tgaccctagc caccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     3421 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     3481 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     3541 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     3601 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     3661 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     3721 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtgtcgccc gaccaccagg
     3781 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     3841 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     3901 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     3961 agcccggtgc ctgaggatct cgagctcgat gagtttggac aaaccacaac tagaatgcag
     4021 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4081 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4141 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtac ttaagaggga
     4201 tcgataagga tctagcttgt ggaaggctac tcgaaatgtt tgacccaagt taaacaattt
     4261 aaaggcaatg ctaccaaata ctaattgagt gtatgtaaac ttctgaccca ctgggaatgt
     4321 gatgaaagaa ataaaagctg aaatgaatca ttctctctac tattattctg atatttcaca
     4381 ttcttaaaat aaagtggtga tcctaactga cctaagacag ggaattttta ctaggattaa
     4441 atgtcaggaa ttgtgaaaaa gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc
     4501 cgacttcaac tgtataggga tcctctagct agagtcgacc tcgagggggg gcccggtacc
     4561 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     4621 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     4681 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     4741 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     4801 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     4861 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5161 cggatacctg tccgcctttc tcccttcggg aaccgtggcg ctttctcata gctcacgctg
     5221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     5281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     5341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     5401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     5461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     5521 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     5581 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     5641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     5701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     5761 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     5821 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     5881 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5941 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6001 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6061 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6121 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6181 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta acttggccgc
     6241 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6301 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6361 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6421 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6481 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6541 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6601 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     6661 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     6721 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     6781 cgcattaagc gcggcgggag tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     6841 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     6901 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     6961 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     7021 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     7081 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     7141 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     7201 aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     7261 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     7321 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     7381 cgcgcgtaat acgactcact atagggcgaa ttggagctcg gatccctata cagttgaagt
     7441 cggaagttta catacactta agttggagtc attaaaactc gtttttcaac tactccacaa
     7501 atttcttgtt aacaaacaat agttttggca agtcagttag gacatctact ttgtgcatga
     7561 cacaagtcat ttttccaaca attgtttaca gacagattat ttcacttata attcactgta
     7621 tcacaattcc agtgggtcag aagtttacat acactaagtt gactgtgcct ttaaacagct
     7681 tggaaaattc cagaaaatga tgtcatggct ttag
//
