LOCUS       sequence-498534-       10316 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  A lentiviral vector express PABP-INT fusion protein under a Tetoff 
            system. Contains EGFP as selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Chao YK, Wu M, Gong Q, Chen F
  TITLE     A genetically encoded device for transcriptome storage in mammalian 
            cells.
  JOURNAL   Science. 2026 Jan 15:eadz9353. doi: 10.1126/science.adz9353.
  PUBMED    41538410
REFERENCE   2  (bases 1 to 10316)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2026 Jan 15:eadz9353. doi: 10.1126/science.adz9353."
COMMENT     Sequence Label: pKC152_Lenti_tetoff_PABP-INT_EF1a_EGFP
FEATURES             Location/Qualifiers
     source          1..10316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3620..3638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3692..3710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3728..3746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3764..3782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3800..3818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3836..3854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3899..3923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4048..4057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5687..5706)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5752..5807
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5806..5825)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        6011..6222
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6235..6503
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      6538..6547
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6544..6546,6547..6549,6550..7260)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6544..6546,6547..6549,6550..7260)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6589..6610)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6850..6869)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7197..7218
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7267..7320
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7321..7938,7939..7944,7945..7980,7981..7983,7984..8019,
                     8020..8022,8023..8058,8059..8067)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIECLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8080..8668
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8133..8153)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8551..8562)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8743..8976
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9095..9113)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9181..9285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9286..9354,9355..10146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9504..9523)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag accggtggcc
     3601 tccacggcca ctagattact ccctatcagt gatagagaac gtatgaagag tttactccct
     3661 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     3721 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     3781 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     3841 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     3901 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     3961 actttccgta ccacttccta ccctcgtaaa gaagttccta ttccgaagtt cctattcttc
     4021 aaaaggtata ggaacttcgc tcgagccgcc accatggcct cgctctacgt gggggacctc
     4081 caccccgacg tgaccgaggc gatgctctac gagaagttca gcccggccgg gcccatcctc
     4141 tccatccggg tctgcaggga catgatcacc cgccgctcct tgggctacgc gtatgtgaac
     4201 ttccagcagc cggcggacgc ggagcgtgct ttggacacca tgaattttga tgttataaag
     4261 ggcaagccag tacgcatcat gtggtctcag cgtgatccat cacttcgcaa aagtggagta
     4321 ggcaacatat tcattaaaaa tctggacaaa tccattgata ataaagcact gtatgataca
     4381 ttttctgctt ttggtaacat cctttcatgt aaggtggttt gtgatgaaaa tggttccaag
     4441 ggctatggat ttgtacactt tgagacgcag gaagcagctg aaagagctat tgaaaaaatg
     4501 aatggaatgc tcctaaatga tcgcaaagta tttgttggac gatttaagtc tcgtaaagaa
     4561 cgagaagctg aacttggagc tagggcaaaa gaattcacca atgtttacat caagaatttt
     4621 ggagaagaca tggatgatga gcgccttaag gatctctttg gcaagtttgg gcctgcctta
     4681 agtgtgaaag taatgactga tgaaagtgga aaatccaaag gatttggatt tgtaagcttt
     4741 gaaaggcatg aagatgcaca gaaagctgtg gatgagatga acggaaagga gctcaatgga
     4801 aaacaaattt atgttggtcg agctcagaaa aaggtggaac ggcagacgga acttaagcgc
     4861 aaatttgaac agatgaaaca agataggatc accagatacc agggtgttaa tctttatgtg
     4921 aaaaatcttg atgatggtat tgatgatgaa cgtctccgga aagagttttc tccatttggt
     4981 acaatcacta gtgcaaaggt tatgatggag ggtggtcgca gcaaagggtt tggttttgta
     5041 tgtttctcct ccccagaaga agccactaaa gcagttacag aaatgaacgg tagaattgtg
     5101 gccacaaagc cattgtatgt agctttagct cagcgcaaag aagagcgcgg tggtagcgtg
     5161 tgcatccagc actggcaaga tgcagttcct tggacggaat tgctgtctct ccagacggag
     5221 gatggtttct ggaagctcac gcccgaactc ggactcatat tgaaccttaa tacaaatggg
     5281 ctccactcct ttctgaagca gaaaggcatc cagagtctcg gtgttaaggg ccgggagtgc
     5341 cttctcgacc tcatagccac gatgctcgtg ttgcaattta tccgcactag gctggaaaaa
     5401 gagggtatcg tattcaaaag tctgatgaaa atggacgacg ctagtataag tagaaacatc
     5461 ccctgggctt ttgaggccat caagcaggcg agtgagtggg tcaggcgaac agagggtcag
     5521 tacccaagca tatgtccccg actggagttg gggaacgact gggatagcgc aaccaagcag
     5581 ctcttgggtc tgcaaccaat ttcaacagtg tcaccactgc atcgagtcct gcattactcc
     5641 cagggctaag gatccaccgg atctagataa ctgagcggcc gcctaatctt tttccctctg
     5701 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     5761 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     5821 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     5881 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     5941 aacagccccc tgctgtccat tccttacata tgactagtaa ggatctgcga tcgctccggt
     6001 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6061 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6121 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6181 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct
     6241 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt
     6301 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt
     6361 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc
     6421 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt
     6481 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacacctgc agccaacgcc
     6541 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     6601 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc
     6661 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     6721 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     6781 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     6841 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     6901 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     6961 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     7021 aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     7081 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     7141 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     7201 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     7261 gctagcgagg gcagaggaag tctgctaaca tgcggtgacg tcgaggagaa tcctggccca
     7321 atgtctagac tggacaagag caaagtcata aactctgctc tggaattact caatgaagtc
     7381 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     7441 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctggcaat cgagtgtctg
     7501 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     7561 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     7621 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     7681 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     7741 acactgggct gcgtattgga ggatcaggag catcaagtag caaaagagga aagagagaca
     7801 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccatcag
     7861 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     7921 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     7981 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     8041 tttgaccttg acatgctccc cggatgagtc gacgatccta atcaacctct ggattacaaa
     8101 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8161 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8221 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8281 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8341 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8401 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8461 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     8521 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8581 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8641 cggatctccc tttgggccgc ctccccgcct gagatccttt aagaccaatg acttacaagg
     8701 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     8761 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     8821 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8881 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8941 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
     9001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggccc gggttaatta
     9061 aggaaagggc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     9121 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     9181 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9241 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9301 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9361 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9421 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9481 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     9541 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9601 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9661 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9721 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9781 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     9841 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     9901 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     9961 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10021 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10081 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10141 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10201 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10261 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
