LOCUS       sequence-498558-        3692 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Encodes UbSYHRE (UBQ-HRE1-GAL4STE12), Gateway compatible as Donor 
            plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Lavilla-Puerta M, He Y, Piccinini L, Di Paco L, Papachristodoulou A,
            Licausi F, Giuntoli B
  TITLE     A synthetic ERFVII-dependent circuit in yeast sheds light on the 
            regulation of early hypoxic responses of plants
  JOURNAL   bioRxiv 2025.09.01.673502
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.01.673502"
COMMENT     Sequence Label: UbSYHRE_pDONR201
FEATURES             Location/Qualifiers
     source          1..3692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     35..54
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     113..132
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    244..343
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(600..1040)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     protein_bind    complement(1781..1880)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1933..2019
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2111..2138
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2273..2861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2353..2372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3004..3692,1..121))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
       61 aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttga atatggctca
      121 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat
      181 ttttatcttg tgcaatgtaa catcagagat tttgagacac gggccagagc tgcagctgga
      241 tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag
      301 caatgctttc ttataatgcc aactttgtac aagaaagctg ggtgttagaa accgttgttg
      361 tttggatcgg ttttcaagaa tggcaaacca tcagacatat catcctcttc agaccaatca
      421 aaaccatgca atgcatctct tggcatgaag tctaatggga tagtagagtt atcatgatga
      481 gcagcagaat caatagctgg agtaaaagct tgtggtggaa atggatcgaa ggcattttct
      541 tcagttggat attcaacaga gactgggaag taatccaatg gaaaatcttc ttcatctcta
      601 gacacggtca actgtctttg acctttgtta gaagattctt cagagctgga ggtagcagaa
      661 attctatgtt gtctcaaggt caatggcata tcagtttcaa cagaagccaa tctatcagta
      721 acggcatcct tgttaacgtt atcttgaacg aacaaaccag tcaacaaagc cttgatatct
      781 tgcaaggaat ccatcttcaa gatcatgtcc agatcttctc ttgggaaaat caacaagaac
      841 agctgctcca atctttccaa cctagattca acttcggtca aatgtgctct agtcaatgga
      901 gatctcttag tctttggaga gtatctacat tcccagttgt tcttcaaaca cttagcacac
      961 tttggctttt ctttggagca cttcaatttt ttcagcctgc agatatcgca agcttgttca
     1021 atagaggaca acagcttcat attggagtct tgatagctcc ataaatttcc aatatcggtc
     1081 gccgattggt cgtcggcaac cggaatctga tagaatctca tgtaatcctc caatgccatc
     1141 aggtcctccg ataagctctc tacttggttt tccgaaatca gctcattttg attcccccct
     1201 ggtttagtat cggcttcttc ttctacttga gtgtttggga aattcagttt ggctttccgg
     1261 ccacggattt tgatggcagc aacgtcgtag gctcgagcag cttcgtcggc ggttttgaat
     1321 gtgccaagcc agacacgtac acctttgctc gggtcacgaa tctccgctgc ccatttgccc
     1381 catggcctct gccttatccc tctgtacaga ttcttcctct ctcgtttccc atctctttct
     1441 tcactcactg agacgggttt acgcttcttc ctgctgctaa cagagccgag ttgactcggt
     1501 tctgactcag atttcgacca gatgaaatca gaaatgatag ctcccccaca accacctctt
     1561 aacctgagaa ccaagtggag tgttgattct ttctggatgt tgtaatcagc caaggtgcgg
     1621 ccatcttcta gctgcttacc agcgaagatc aatctttgct ggtcaggagg aatgccttcc
     1681 ttgtcctgga tctttgcctt aacattgtcg atggtgtcag agctctcaac ctcgagagtg
     1741 atggtcttac cagtgagtgt tttcacgaaa atttgcatgg agcctgcttt tttgtacaaa
     1801 gttggcatta taaaaaagca ttgctcatca atttgttgca acgaacaggt cactatcagt
     1861 caaaataaaa tcattatttg gggcccgaga tccatgctag cgttaacgcg agagtaggga
     1921 actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
     1981 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     2041 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     2101 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct
     2161 ggctagcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2221 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2281 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2341 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2401 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2461 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2521 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2581 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2641 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2701 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2761 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2821 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2881 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagct
     2941 tgcgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
     3001 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
     3061 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
     3121 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
     3181 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
     3241 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
     3301 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
     3361 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
     3421 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
     3481 atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga gtaaccatgc
     3541 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
     3601 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
     3661 aaacaactct ggcgcatcgg gcttcccata ca
//
