LOCUS       sequence-498564-        9333 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Lentiviral expression of human NUDT5 W28A Y74E with N-terminal 
            mCherry seperated by a flexible GSG-linker and P2A, under CMV 
            promoter as well as a puromycin resistance cassette under PGK 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Nguyen TA, Lin JG, Marques AMC, Fottner M, Bauer LG, Reicher A, Daum
            D, Scrofani L, Liu Y, Cheng C, D'Angelo L D D L, Sanchez J, Bueschl 
            C, Marella N, Buphamalai P, Traversi F, Beres M, Moll HP, Siklos M, 
            Genger JW, Hofstaetter G, Villanti L, Malik M, Klimek C, 
            Runggatscher K, Guertl B, Hansen JS, Dobner S, Babosova O, Becirovic
            T, de Rooij LPMH, Casanova E, Koren A, Froese DS, Rosenblatt DS, 
            Klavins K, Bergthaler A, Menche J, Hannich JT, Abele M, Sdelci S, 
            Lang K, Huber KVM, Kubicek S
  TITLE     A non-enzymatic role of Nudix hydrolase 5 in repressing purine de 
            novo synthesis.
  JOURNAL   Science. 2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257.
  PUBMED    41196952
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257."
COMMENT     Sequence Label: Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A Y74E-WPRE
FEATURES             Location/Qualifiers
     source          1..9333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..708
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(146..164)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             718..774
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    1450..2038
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1503..1523)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1921..1932)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        2052..2551
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2087..2110)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2484..2503
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2572..3171
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2572..2591)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3068..3088
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3309..3542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3614..3735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3651..3670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3705..3724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3775..3910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3930..3949)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3931..3949)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3959..3976)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3959..3975)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3968..3990)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4117..4572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4204..4223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4414..4435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4598..4702
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4703..4771,4772..5563)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4921..4940)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5734..6322
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6223..6242
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6476..6493
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6610..6631
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6646..6651,6652..6669,6670..6676)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6684..6700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6689..6711
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6708..6724
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6708..6724
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6743..6763
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6745..6763
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6791..7017
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7018..7198
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7245..7370
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7863..8096
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8281..8325
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8592..8708
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8731..9034
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9035..9238
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9189..9209
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9235..9259
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      join(9328..9333,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
       61 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      121 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
      181 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
      241 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
      301 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      361 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctcaga cggccccgta
      421 atgcagaaga aaaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
      481 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
      541 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
      601 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      661 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg atccggcgct
      721 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatggag
      781 agccaagaac caacggaatc ttctcagaat ggcaaacagt atatcatttc agaggagtta
      841 atttcagaag gaaaagcagt caagcttgaa aaaacaacgt acatggatcc tactggtaaa
      901 actagaactt gggaatcagt gaaacgtaca accaggaaag agcagactgc ggatggtgtc
      961 gcggtcatcc ccgtgctgca gagaacactt cacgaagagt gtatcgttct ggtgaaacag
     1021 ttccgaccac caatgggggg ctactgcata gagttccctg caggtctcat agatgatggt
     1081 gaaaccccag aagcagctgc tctccgggag cttgaagaag aaactggcta caaaggggac
     1141 attgccgaat gttctccagc ggtctgtatg gacccaggct tgtcaaactg tactatacac
     1201 atcgtgacag tcaccattaa cggagatgat gccgaaaacg caaggccgaa gccaaagcca
     1261 ggggatggag agtttgtgga agtcatttct ttacccaaga atgacctgct gcagagactt
     1321 gatgctctgg tagctgaaga acatctcaca gtggacgcca gggtctattc ctacgctcta
     1381 gcgctgaaac atgcaaatgc aaagccattt gaagtgccct tcttgaaatt ttaaagcggc
     1441 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     1501 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     1561 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     1621 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     1681 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     1741 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     1801 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     1861 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     1921 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     1981 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     2041 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     2101 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     2161 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     2221 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     2281 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     2341 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     2401 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     2461 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     2521 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     2581 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     2641 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     2701 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     2761 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     2821 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     2881 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     2941 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     3001 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     3061 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     3121 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     3181 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
     3241 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     3301 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     3361 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3421 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3481 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3541 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     3601 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3661 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3721 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     3781 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3841 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3901 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     3961 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4021 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4081 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4141 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4201 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4261 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4321 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4381 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4441 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4501 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4561 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4621 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4681 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4741 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4801 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4861 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4921 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     4981 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5041 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5101 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5161 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5221 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5281 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5341 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5401 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5461 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5521 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5581 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5641 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5701 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5761 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5821 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5881 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5941 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6001 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6061 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6121 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6181 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6241 ttatagtcct gtcgggtttc gccacctctt acttgagcgt cgatttttgt gatgctcgtc
     6301 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6361 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6421 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6481 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6541 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6601 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6661 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6721 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     6781 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     6841 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     6901 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     6961 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     7021 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7081 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7141 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7201 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7261 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7321 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7381 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7441 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7501 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7561 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7621 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7681 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7741 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7801 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7861 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     7921 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     7981 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8041 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8101 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8161 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8221 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8281 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8341 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8401 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8461 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     8521 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     8581 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     8641 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc
     8701 aaaattttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
     8761 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     8821 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     8881 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     8941 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     9001 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     9061 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     9121 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     9181 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
     9241 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     9301 acaccgactc tagaggatcc accggtcgcc acc
//
