LOCUS       sequence-498565-        8620 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Lentiviral expression of human NUDT5 with N-terminal 2xHA under CMV 
            promoter and puromycin resistance cassette under PGK promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8620)
  AUTHORS   Nguyen TA, Lin JG, Marques AMC, Fottner M, Bauer LG, Reicher A, Daum
            D, Scrofani L, Liu Y, Cheng C, D'Angelo L D D L, Sanchez J, Bueschl 
            C, Marella N, Buphamalai P, Traversi F, Beres M, Moll HP, Siklos M, 
            Genger JW, Hofstaetter G, Villanti L, Malik M, Klimek C, 
            Runggatscher K, Guertl B, Hansen JS, Dobner S, Babosova O, Becirovic
            T, de Rooij LPMH, Casanova E, Koren A, Froese DS, Rosenblatt DS, 
            Klavins K, Bergthaler A, Menche J, Hannich JT, Abele M, Sdelci S, 
            Lang K, Huber KVM, Kubicek S
  TITLE     A non-enzymatic role of Nudix hydrolase 5 in repressing purine de 
            novo synthesis.
  JOURNAL   Science. 2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257.
  PUBMED    41196952
REFERENCE   2  (bases 1 to 8620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2025 Nov 6:eadv4257. doi: 10.1126/science.adv4257."
COMMENT     Sequence Label: Lenti-CMV-2xHA-NUDT5-WPRE
FEATURES             Location/Qualifiers
     source          1..8620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        92..295
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     246..266
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     292..316
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             395..421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             395..421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             425..451
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1127..1715
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1180..1200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1598..1609)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        1729..2228
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1764..1787)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2161..2180
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2249..2848
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2249..2268)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2745..2765
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2986..3219
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3291..3412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3328..3347)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3382..3401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3452..3587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3607..3626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3608..3626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3636..3653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3636..3652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3645..3667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3794..4249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3881..3900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4091..4112
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4275..4379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4380..4448,4449..5240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4598..4617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5411..5999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5900..5919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6153..6170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6287..6308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6323..6328,6329..6346,6347..6353)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6361..6377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6366..6388
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6385..6401
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6385..6401
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6420..6440
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6422..6440
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6468..6694
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6695..6875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6922..7047
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7540..7773
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7958..8002
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8269..8385
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(8408..8620,1..91)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
       61 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      121 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      181 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      241 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcgttt
      301 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
      361 ccgactctag agggatccac cggtcgccac catgtacccc tacgacgtgc ccgactacgc
      421 cggctatccg tatgatgtcc cggactatgc aggagagagc caagaaccaa cggaatcttc
      481 tcagaatggc aaacagtata tcatttcaga ggagttaatt tcagaaggaa aatgggtcaa
      541 gcttgaaaaa acaacgtaca tggatcctac tggtaaaact agaacttggg aatcagtgaa
      601 acgtacaacc aggaaagagc agactgcgga tggtgtcgcg gtcatccccg tgctgcagag
      661 aacacttcac tatgagtgta tcgttctggt gaaacagttc cgaccaccaa tggggggcta
      721 ctgcatagag ttccctgcag gtctcataga tgatggtgaa accccagaag cagctgctct
      781 ccgggagctt gaagaagaaa ctggctacaa aggggacatt gccgaatgtt ctccagcggt
      841 ctgtatggac ccaggcttgt caaactgtac tatacacatc gtgacagtca ccattaacgg
      901 agatgatgcc gaaaacgcaa ggccgaagcc aaagccaggg gatggagagt ttgtggaagt
      961 catttcttta cccaagaatg acctgctgca gagacttgat gctctggtag ctgaagaaca
     1021 tctcacagtg gacgccaggg tctattccta cgctctagcg ctgaaacatg caaatgcaaa
     1081 gccatttgaa gtgcccttct tgaaatttta aagcggccgc gtcgacaatc aacctctgga
     1141 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     1201 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     1261 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     1321 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     1381 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     1441 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     1501 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     1561 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     1621 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     1681 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg gtaggggagg
     1741 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     1801 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     1861 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     1921 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     1981 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     2041 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     2101 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     2161 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     2221 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     2281 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     2341 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     2401 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     2461 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     2521 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     2581 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     2641 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     2701 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     2761 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     2821 gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga
     2881 aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca atgacttaca
     2941 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctagctc
     3001 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3061 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3121 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3181 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     3241 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3301 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3361 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3421 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3601 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3661 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     3721 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     3781 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3841 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3901 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3961 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4021 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4081 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4141 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4201 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     4921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     4981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5521 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6181 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6301 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6361 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6421 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6481 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6541 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6601 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6661 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     6721 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6781 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6841 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     6901 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     6961 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7021 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7081 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7141 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7201 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7261 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7321 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7381 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7441 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7501 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7561 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7621 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7681 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     7741 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     7801 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     7861 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     7921 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     7981 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8041 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8101 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8161 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     8221 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
     8281 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     8341 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga taagcttggg
     8401 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     8461 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     8521 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     8581 atatgccaag tacgccccct attgacgtca atgacggtaa
//
