LOCUS       sequence-498571-        5836 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  Urease Cluster KO | Erythromycin Resistance Cassette | Conjugative 
            Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ortiz L, Esmurria A, Gilbert C, Crits-Christoph A, Barnum TP, 
            Mancuso CP, Kang SC, Leung J, Fenn K, Abrams MB, Brumwell SL, Lee 
            HH, Ostrov N
  TITLE     A genetic platform for a biocementation bacterium
  JOURNAL   bioRxiv 2025.12.05.692644
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.12.05.692644"
COMMENT     Sequence Label: pGLU_504
FEATURES             Location/Qualifiers
     source          1..5836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            3..111
                     /note="incP origin of transfer"
     primer_bind     complement(3760..3776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3760..3776)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3773..3795)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      3966..4354
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(4487..4511)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4611..5402,5403..5471))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5234..5253
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5472..5576)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5675..5697
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5689..5706
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5690..5706
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tacggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca
       61 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc
      121 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg
      181 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg
      241 gaaaagtaat cattcgtata tatgtacggg cttcatcttt tttgttaatc aaggttggta
      301 gtgcgtgtaa aatcgcatat tttatacata aaaatgaggc aacacttgtt ttgtaacagt
      361 gtagatggag aggcatcaag gttttagccg attatggcaa aaatcttgat gtttttttag
      421 tttttgggca taataaataa actgatttag caagaaatct atgcaattaa gtttatatag
      481 aaggtgacgc ctaaactttt tcgtgtagtt tgttcagttt atagtcaaaa tgcggaagcg
      541 aaactaggtc ttcacacctt tgcgctctct ttttacaggt ttttcaaacc cgtaaacttc
      601 ctttgtaatt cggttataaa tcataaatat cactcacttt ctaaggtttt taatggagtg
      661 tgtttaggaa ttttcgaaag gcagtctagt gaaagtattc ggaagaattt tgttttgaac
      721 attttttagt ggaatccttt taaagaattt agatttatgt tatacatata ttcgccgggt
      781 cttttctata gtgtttagtt taccaaatcc actagacaag aaaagatccc attttcatgt
      841 ttatgaatct atcgtgcatt cgtttggctt ctagttcatt aaatgaattc ggatgctatc
      901 taaatcgcca ataaccattt tactgatcat gtatgatgtc aaaatggtta tccgtatgat
      961 tactctctag cctgacgata aagtgatttt aatttatttg ttatcattat ggctaatttt
     1021 gataaagcaa ccataatgag tatagcaacc taaatttaaa tcattccgtc aaaaatcgtg
     1081 aattagcctg aatttcggaa cgtctattcc ttaatttgat atatacgatt acctattttc
     1141 aaattgatca tcgtactaaa aaagtctcgc ttaattttat tgaaaatagg tttgatttaa
     1201 ggtggtttgg ggaaggtatg ttcaaagcgc tttagaagtt aggagaccag cttcacacgg
     1261 cgtgtggctg cggaacgggt cacaaaaagc tctgactgcc ggtagcgttc ctaaacatgc
     1321 attaccctac tcgacgcgtc ctacgcgtga cccgatcctt taactctggc aaccctcaaa
     1381 attgaatgag acatgctaca cctccggata ataaatatat ataaacgtat atagatttca
     1441 taaagtctaa cacactagac ttatttactt cgtaattaag tcgttaaacc gtgtgctcta
     1501 cgaccaaaac tataaaacct ttaagaactt tcttttttta caagaaaaaa gaaattagat
     1561 aaatctctca tatcttttat tcaataatcg catccgattg cagtataaat ttaacgatca
     1621 ctcatcatgt tcatatttat cagaggtcgt gctataatta tactaatttt ataaggagga
     1681 aaaaatatgg gcatttttag tatttttgta atcagcacag ttcattatca accaaacaaa
     1741 aaataagtgg ttataatgaa tcgttaataa gcaaaattca tataaccaaa ttaaagaggg
     1801 ttataaatga acgagaaaaa tataaaacac agtcaaaact ttattacttc taagcataat
     1861 atcgacaaga ttatgacaaa cataagatta aatgaacatg ataatatctt tgaaatcggc
     1921 tcaggaaaag gccattttac ccttgaatta gtaaagaggt gtaatttcgt aactgccatt
     1981 gaaatagacc ataaattatg caaaactaca gaaaataaac ttgttgatca cgataatttc
     2041 caagttttaa acaaggatat attgcagttt aaatttccta aaaaccaatc ctataaaata
     2101 tatggtaata taccttataa cataagtacg gatataatac gcaaaattgt ttttgatagt
     2161 atagctaatg agatttattt aatcgtggaa tacgggtttg ctaaaagatt attaaataca
     2221 aaacgctcat tggcattact tttaatggca gaagttgata tttctatatt aagtatggtt
     2281 ccaagagaat attttcatcc taaacctaaa gtgaatagct cacttatcag attaagtaga
     2341 aaaaaatcaa gaatatcaca caaagataaa caaaagtata attatttcgt tatgaaatgg
     2401 gttaacaaag aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat
     2461 gcaggaattg acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc
     2521 tataaattat ttaataagta agcttgttaa gggatgcata aactgcatcc cttaacttgt
     2581 ttttcgtgtg cctatttttt gtgaatcgat tatgtctttt gcgcagtcgg cattataaaa
     2641 taacgagtct cattggggac ggtcactgtc ttacaatgag atgagatttt ataatggtgc
     2701 acctattgta aaatacaaat tataaacaac actctatcca cggagaggca ttggctttca
     2761 aacgataatt gtaaaatttg ttatgcgcat atgaacagct agttagtgaa tgattttatg
     2821 cgcaaaacat gatacttcac actaaaagaa agcctacata cctaattctc cttgagtggg
     2881 ataggttgtt tatagaagtt atggaaaatt gtgtaactat agtttgccgt tcaatcgagt
     2941 tcgaaattat cgtgtttcat tgtgaaagta ttcacattga aataacgttc atctggggag
     3001 atgttcggat atgattatta accaaaacga tgagcataca aatctatagt atttacaaca
     3061 accttttgca atatatttaa gatttaatat tatttataca attttaaaat acttggagtg
     3121 aaaaatatgt ctaataatgc tcaaacagca caacaaggtg gatcaaatgg caaggcgtat
     3181 ctaatcctaa tcattggtat tatggcagtt gcgttttcag caccttgggt aaagcaatcg
     3241 aactttgaac cagcaacttc tgttgttctt cgtgttggtt ttggattgtt gttcttgcta
     3301 cctttcttta taagagaaat taaaaaaatc ggtaaaataa acaaaaccgg tgttatttta
     3361 tctcttgttg caggtttgtt tcttggaatt gactttacag cttggaatta ttcaatctat
     3421 ttcgtaggat cagggattgc atcaatctta ctgaacctac aaatcatcat tttaccggca
     3481 cttgctttcc tattcgatag agagcgtatt ccaaaaagtt tcttcatcgt tgcgccaatc
     3541 atgattcttg gtgtactcat gacaggtggt attttcgaca gtgctgaagc gactgaagga
     3601 ccaacgactg catggggtat gaaccttgcc gccaggatac atagattacc acaactccga
     3661 gcccttccac ctagcgtgct aaccacgcac gctattgttg tataggctag gtggaggctc
     3721 agtgatgata agtctgcgat ggtggatgca tgtgtcatgg tcatagctgt ttcctgtgtg
     3781 aaattgttat ccgctcagag ggcacaatcc tattccgcgc tatccgacaa tctccaagac
     3841 attaggtgga gttcagttcg gcgtatggca tatgtcgctg gaaagaacat gtgagcaaaa
     3901 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttaca gctgggcgcg
     3961 ccccatgtca gccgttaagt gttcctgtgt cactcaaaat tgctttgaga ggctctaagg
     4021 gcttctcagt gcgttacatc cctggcttgt tgtccacaac cgttaaacct taaaggcttt
     4081 aaaagcctta tatattcttt tttttcttat aaaacttaaa accttagagg ctatttaagt
     4141 tgctgattta tattaatttt attgttcaaa catgagagct tagtacgtga aacatgagag
     4201 cttagtacgt tagccatgag agcttagtac gttagccatg agggtttagt tcgttaaaca
     4261 tgagagctta gtacgttaaa catgagagct tagtacgtga aacatgagag cttagtacgt
     4321 actatcaaca ggttgaactg ctgatcttca gatcggcgcg ccggccggcc gaagatcctt
     4381 tgatcttttc tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc
     4441 gtaaatgggt agggggcttc aaatcgtcct cgtgatacca attcggagcc tgcttttttg
     4501 tacaaacttg ttgataatgg caattcaagg atcttcacct agatcctttt aaattaaaaa
     4561 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     4621 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     4681 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     4741 atgataccgc gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     4801 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     4861 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     4921 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4981 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5041 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5101 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5161 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5221 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5281 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5341 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5401 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5461 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     5521 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     5581 tttccccgaa aagtgccaga tacctgaaac aaaacccatc gtacggccaa ggaagtctcc
     5641 aataactgtg atccaccaca agcgccaggg ttttcccagt cacgacgttg taaaacgacg
     5701 gccagtcatg cataatccgc acgcatctgg aataaggaag tgccattccg cctgacctta
     5761 gcgtgcgaac agcacgctat tgttgtatca ttactcgcat ccattctcag gctgtctcgt
     5821 ctcgtctccg aaggag
//
