LOCUS       sequence-498577-       10504 bp    DNA     circular SYN 28-JAN-2026
DEFINITION  lentiviral vector for expressing Halo-tagged RNase L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  TITLE     Halo-tagged RNase L with or without cytoplasmic retention signal
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 28, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLJM1-Halo-RNaseL
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        131..334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     285..305
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      390..399
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             396..1286
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     misc_feature    3587..3704
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3753..4263
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4182..4200
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4285..4884
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4285..4304)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4781..4801
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5012..5245
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5317..5438
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5354..5373)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5408..5427
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5478..5613
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5633..5652)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5634..5652)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5662..5679)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5662..5678)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5671..5693)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5820..6275
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5907..5926)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6117..6138
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6301..6405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6406..6474,6475..7266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6624..6643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7437..8025
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7926..7945
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8179..8196
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8313..8334
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8349..8354,8355..8372,8373..8379)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8387..8403
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8392..8414
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8411..8427
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8411..8427
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8446..8466
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8448..8466
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8494..8720
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8721..8901
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8948..9073
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9566..9799
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9984..10028
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     10326..10346
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
       61 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      301 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      361 gcgctaccgg tgtcgacgat atctctagag ccaccatggc agaaatcggt actggctttc
      421 cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc gatgttggtc
      481 cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc tacgtgtggc
      541 gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac ctgatcggta
      601 tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc cgcttcatgg
      661 atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac gactggggct
      721 ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt attgcattta
      781 tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc cgcgagacct
      841 tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag aacgttttta
      901 tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag atggaccatt
      961 accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc ccaaacgagc
     1021 tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac atggactggc
     1081 tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt ctgatcccac
     1141 cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg gacatcggcc
     1201 cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag atcgcgcgct
     1261 ggctgtcgac gctcgagatt tccggatccg ctggctccgc tgctggttct ggcgaattca
     1321 tggagagcag ggatcacaac aacccccagg agggacccac gtcctccagc ggtagaaggg
     1381 ctgcagtgga agacaatcac ttgctgatta aagctgttca aaacgaagat gttgacctgg
     1441 tccagcaatt gctggaaggt ggagccaatg ttaatttcca ggaagaggaa gggggctgga
     1501 cacctctgca taacgcagta caaatgagca gggaggacat tgtggaactt ctgcttcgtc
     1561 atggtgctga ccctgttctg aggaagaaga atggggccac gccttttatc ctcgcagcga
     1621 ttgcggggag cgtgaagctg ctgaaacttt tcctttctaa aggagcagat gtcaatgagt
     1681 gtgattttta tggcttcaca gccttcatgg aagccgctgt gtatggtaag gtcaaagccc
     1741 taaaattcct ttataagaga ggagcaaatg tgaatttgag gcgaaagaca aaggaggatc
     1801 aagagcggct gaggaaagga ggggccacag ctctcatgga cgctgctgaa aaaggacacg
     1861 tagaggtctt gaagattctc cttgatgaga tgggggcaga tgtaaacgcc tgtgacaata
     1921 tgggcagaaa tgccttgatc catgctctcc tgagctctga cgatagtgat gtggaggcta
     1981 ttacgcatct gctgctggac catggggctg atgtcaatgt gaggggagaa agagggaaga
     2041 ctcccctgat cctggcagtg gagaagaagc acttgggttt ggtgcagagg cttctggagc
     2101 aagagcacat agagattaat gacacagaca gtgatggcaa aacagcactg ctgcttgctg
     2161 ttgaactcaa actgaagaaa atcgccgagt tgctgtgcaa acgtggagcc agtacagatt
     2221 gtggggatct tgttatgaca gcgaggcgga attatgacca ttcccttgtg aaggttcttc
     2281 tctctcatgg agccaaagaa gattttcacc ctcctgctga agactggaag cctcagagct
     2341 cacactgggg ggcagccctg aaggatctcc acagaatata ccgccctatg attggcaaac
     2401 tcaagttctt tattgatgaa aaatacaaaa ttgctgatac ttcagaagga ggcatctacc
     2461 tggggttcta tgagaagcaa gaagtagctg tgaagacgtt ctgtgagggc agcccacgtg
     2521 cacagcggga agtctcttgt ctgcaaagca gccgagagaa cagtcacttg gtgacattct
     2581 atgggagtga gagccacagg ggccacttgt ttgtgtgtgt caccctctgt gagcagactc
     2641 tggaagcgtg tttggatgtg cacagagggg aagatgtgga aaatgaggaa gatgaatttg
     2701 cccgaaatgt cctgtcatct atatttaagg ctgttcaaga actacacttg tcctgtggat
     2761 acacccacca ggatctgcaa ccacaaaaca tcttaataga ttctaagaaa gctgctcacc
     2821 tggcagattt tgataagagc atcaagtggg ctggagatcc acaggaagtc aagagagatc
     2881 tagaggacct tggacggctg gtcctctatg tggtaaagaa gggaagcatc tcatttgagg
     2941 atctgaaagc tcaaagtaat gaagaggtgg ttcaactttc tccagatgag gaaactaagg
     3001 acctcattca tcgtctcttc catcctgggg aacatgtgag ggactgtctg agtgacctgc
     3061 tgggtcatcc cttcttttgg acttgggaga gccgctatag gacgcttcgg aatgtgggaa
     3121 atgaatccga catcaaaaca cgaaaatctg aaagtgagat cctcagacta ctgcaacctg
     3181 ggccttctga acattccaaa agttttgaca agtggacgac taagattaat gaatgtgtta
     3241 tgaaaaaaat gaataagttt tatgaaaaaa gaggcaattt ctaccagaac actgtgggtg
     3301 atctgctaaa gttcatccgg aatttgggag aacacattga tgaagaaaag cataaaaaga
     3361 tgaaattaaa aattggagac ccttccctgt attttcagaa gacatttcca gatctggtga
     3421 tctatgtcta cacaaaacta cagaacacag aatatagaaa gcatttcccc caaacccaca
     3481 gtccaaacaa gcctcagtgt gatggagctg gtggggccag tgggttggcc agccctgggt
     3541 gctgagaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     3601 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     3661 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     3721 acagcagaga tccactttgg ccgcggctcg agggggttgg ggttgcgcct tttccaaggc
     3781 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     3841 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     3901 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     3961 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     4021 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     4081 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     4141 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     4201 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     4261 cagggggatc caccggagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     4321 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     4381 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4441 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4501 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4561 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4621 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4681 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4741 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4801 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4861 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     4921 agcgcacgac cccatgcatc ggtaccttta agaccaatga cttacaaggc agctgtagat
     4981 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5041 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5101 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5161 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5221 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     5281 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5341 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5401 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5461 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5521 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5581 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     5641 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     5701 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     5761 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     5821 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     5881 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     5941 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6001 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6061 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6121 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6181 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6241 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     6301 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6361 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6421 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6481 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6541 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6601 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6661 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6721 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6781 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6841 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6901 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6961 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     7021 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     7081 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     7141 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7201 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7261 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7321 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7381 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7441 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7501 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7561 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7621 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7681 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7741 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7801 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7861 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7921 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7981 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     8041 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     8101 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     8161 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8221 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     8281 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     8341 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     8401 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     8461 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     8521 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     8581 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     8641 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     8701 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     8761 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     8821 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     8881 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     8941 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     9001 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     9061 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     9121 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     9181 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     9241 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     9301 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     9361 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     9421 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     9481 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     9541 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     9601 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     9661 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     9721 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     9781 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     9841 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     9901 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     9961 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
    10021 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
    10081 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
    10141 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
    10201 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
    10261 gattagtgaa cggatctcga cggtatcgat cacgagacta gcctcgagcg gccgccccct
    10321 tcaccgaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
    10381 gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
    10441 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
    10501 atca
//
