LOCUS       sequence-498751-        7025 bp    DNA     circular SYN 30-JAN-2026
DEFINITION  Photostable genetically encoded voltage indicator for imaging in rat
            hippocampal neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Cao C, Ruixin Zhu, Zhou S, Zhao Z, Lin C, Liu S, Peng L, Subach FV, 
            Piatkevich KD, Zou P
  TITLE     A photostable genetically encoded voltage indicator for imaging 
            neural activities in tissue and live animals
  JOURNAL   bioRxiv 2025.05.29.656768
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jan 30, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.05.29.656768"
COMMENT     Sequence Label: pAAV-hSyn-Vega
FEATURES             Location/Qualifiers
     source          1..7025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        341..788
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2198..2902
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGF
                     QTAKLKVTKGGPLPFAWDILSPHFTFGSKAYVKHPADIPDYFKLSFPEGFKWERVMNYE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     KIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    3158..3746
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3211..3231)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3629..3640)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3766..4242
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3965..3984)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4288..4428
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4288..4417
                     /label=AAV2 ITR
     rep_origin      4503..4958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4590..4609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4800..4821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4975..4994)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5094..5116
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5154..5172)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5240..5344
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5345..5413,5414..6205)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5563..5582)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6376..6964
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6865..6884
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgcaaa gatggataaa gttttaaaca gagaggaatc
      181 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
      241 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcagca
      301 aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg ggttttagga
      361 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
      421 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
      481 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
      541 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
      601 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
      661 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
      721 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
      781 gagcgcagtc gagaagggga tggctgacgt ggaaaccgag accggcatga ttgcacagtg
      841 gattgtcttt gctattatgg ctgctgctgc tattgctttt ggagtggctg tgcactttcg
      901 gccttcagag ctgaagagcg catactatat caacattgcc atctgcacta tcgccgctac
      961 cgcttactat gcaatggccg tgaactacca ggacctgaca atgggaagcg ccagtactcc
     1021 cttcaagttt cagctcaaag ggaccatcaa tgggaagagc ttcactgttg agggagaggg
     1081 cgaaggaaac tctcatgagg gttctcacaa ggggaaatat gtctgcacat ctggaaagct
     1141 tcccatgtca tgggcagcct tgggcacaag tttcggctat ggcatgaagt actacacgaa
     1201 atacccatcc ggtctgaaga actggtttcg agaggtgatg cctggtggct ttacctatga
     1261 caggcatatc cagtacaagg gagatggatc cattcatgcg aagcaccagc acttcatgaa
     1321 gaatgggacg taccacaaca ttgtggagtt taccggtcaa gacttcaagg agaatagccc
     1381 ggtgttgaca ggcgatatga atgtgagcct tcccaacgaa gtccctcaca tacctagaga
     1441 tgatggcgtt gagtgtccag tcacactcct gtacccactg ctgagcgaca agtccaagta
     1501 tgtggaagcc tatcagtaca ccatctgcaa accgctgcac aatcaaccag ctcctgatgt
     1561 gccctatcac tggattcgga aacagtatac tcagagcaaa gatgacgcag aagaacgcga
     1621 ccatatctgt cagtcagaaa ccctggaagc tcattccgga aatggtgaaa ggcaggtggt
     1681 ctacgcaaga tatatttgtt gggtgctgac cacaccactg ctcctgctcg atctcatcgt
     1741 catgaccaag atgggcggag tgatgatttc ttgggtcatc ggcgcagaca ttttcatgat
     1801 cgtgtttggt attctgggcg ccttcgagga tgaacacaag ttcaaatggg tgtactttat
     1861 cgctggatgt gtgatgcagg cagtcctgac atacgggatg tataacgcca cttggaaaga
     1921 cgatctgaag aaaagccccg agtaccatag ctcctatgtc agtctgctcg tcttcctgtc
     1981 aatcctctgg gtgttttatc ctgtcgtgtg ggctttcggg tctggtagtg gcgtgctgtc
     2041 cgtcgacaat gaggccattc tcatgggaat cctggatgtg ctcgctaagc cactgtttgg
     2101 aatggggtgc ctcattgccc atgagactat cttcaagaag agtagaatca caagcgaagg
     2161 cgagtacatc cccctggatc aaatagacat aaatgtagtg agcaagggcg aggagaacaa
     2221 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
     2281 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc
     2341 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     2401 tttcaccttt ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa
     2461 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt
     2521 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     2581 gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
     2641 ggcctcctcc gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag
     2701 gctgaagctg aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa
     2761 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca
     2821 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     2881 cggcatggac gagctgtaca aggctagcca gagccagcct atcctgaaca ccaaggagat
     2941 ggcccctcag agcaagcctc ctgaggagct ggagatgagc agcatgccta gccctgtggc
     3001 ccctctgcct gccaggaccg agggcgtgat cgacatgagg agcatgagca gcatcgacag
     3061 cttcatcagc tgcgccaccg acttccctga ggccaccagg ttcgggggct tctgctacga
     3121 gaacgaggtg taagaattcg atatcaagct tatcgataat caacctctgg attacaaaat
     3181 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     3241 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     3301 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     3361 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     3421 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     3481 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     3541 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tatgttgcca cctggattct
     3601 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     3661 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     3721 gatctccctt tgggccgcct ccccgcatcg ataccggctc gagacgggtg gcatccctgt
     3781 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3841 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3901 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3961 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     4021 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     4081 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     4141 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     4201 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctcgagct gattttgtag
     4261 gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
     4321 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4381 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     4441 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     4501 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4561 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4681 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     4741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4801 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     4861 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     4921 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     4981 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5041 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5101 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5161 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5221 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5281 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5341 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5401 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5461 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5521 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5581 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5641 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5701 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5761 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5821 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5881 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5941 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6001 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6061 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6121 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6181 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6241 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6301 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6361 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6421 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6481 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6541 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6601 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6661 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6721 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6781 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6841 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6901 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6961 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7021 catgt
//
