LOCUS       sequence-498802-        7107 bp    DNA     circular SYN 02-FEB-2026
DEFINITION  Based on pAF259 but with bitluc expression driven by the C. 
            saccharoperbutylacetonicum bdh promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7107)
  TITLE     Fagan lab plasmids 2025
REFERENCE   2  (bases 1 to 7107)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEW001
FEATURES             Location/Qualifiers
     source          1..7107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     oriT            1951..2060
                     /note="incP origin of transfer"
     CDS             2093..2464
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttcaaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgctcggc
      781 cggggatcga tccccgggta cgtacccggc agtttttctt tttcggcaag tgttcaagaa
      841 gttattaagt cgggagtgca gtcgaagtgg gcaagttgaa aaattcacaa aaatgtggta
      901 taatatcttt gttcattaga gcgataaact tgaatttgag agggaactta gatggtattt
      961 gaaaaaattg ataaaaatag ttggaacaga aaagagtatt ttgaccacta ctttgcaagt
     1021 gtaccttgta cctacagcat gaccgttaaa gtggatatca cacaaataaa ggaaaaggga
     1081 atgaaactat atcctgcaat gctttattat attgcaatga ttgtaaaccg ccattcagag
     1141 tttaggacgg caatcaatca agatggtgaa ttggggatat atgatgagat gataccaagc
     1201 tatacaatat ttcacaatga tactgaaaca ttttccagcc tttggactga gtgtaagtct
     1261 gactttaaat catttttagc agattatgaa agtgatacgc aacggtatgg aaacaatcat
     1321 agaatggaag gaaagccaaa tgctccggaa aacattttta atgtatctat gataccgtgg
     1381 tcaaccttcg atggctttaa tctgaatttg cagaaaggat atgattattt gattcctatt
     1441 tttactatgg ggaaatatta taaagaagat aacaaaatta tacttccttt ggcaattcaa
     1501 gttcatcacg cagtatgtga cggatttcac atttgccgtt ttgtaaacga attgcaggaa
     1561 ttgataaata gttaacttca ggtttgtctg taactaaaaa caagtattta agcaaaaaca
     1621 tcgtagaaat acggtgtttt ttgttaccct aaaatctaca attttataca taaccacagg
     1681 ttagtacaaa gaccttgtgt ttctttttga aaggcttaaa acaaggattt ttccttgatt
     1741 taagccccga aaagcaacac aaccaaggtt ttagtatcaa tctgtggttt ttatattttc
     1801 agagaccggt cagatctgca gaattcgccc ttcctgcttc ggggtcatta tagcgatttt
     1861 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
     1921 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
     1981 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
     2041 tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga
     2101 tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga
     2161 acgaagagcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct
     2221 ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct
     2281 ggcccgcatc aatggcgacc tgggccgcct gggcggcctg ctgaaactct ggctcaccga
     2341 cgacccgcgc acggcgcggt tcggtgatgc cacgatcctc gccctgctgg cgaagatcga
     2401 agagaagcag gacgagcttg gcaaggtcat gatgggcgtg gtccgcccga gggcagagcc
     2461 atgacttttt tagccgctaa aacggccggg gggtgcgcgt gattgccaag cacgtcccca
     2521 tgcgctccat caagaagagc gacttcgcgg agctggtgaa gtacatcacc gacgagcaag
     2581 gcaagaccga tcccctacta ctgacagctt ccaaggagct aaagaggtcc ctagcgcagc
     2641 ctaagggcga attccagcac actggcatct ttttatttag ggatttctca cataaaatag
     2701 aggctattta tagcctctat tttattttat atttatttta aataaaaaag acttctcatg
     2761 agagaagcct ttttctattt aaagttttat taaaacttat aggatcccta tagaatttct
     2821 tcaaaaagtc tataacctgt aacactgttt atagttactc taaacaacat agatccatca
     2881 ggtgtaatta acctttcatc tattatttta tttccattcc acaaagtgcc tgttacggtt
     2941 attttctttc catcaaaaac tgcaatccct tcgtatggtc taccaaaata atttaacata
     3001 tttggtgtta caccatcaat tactaaagtt ccataaggta atattacttt aaagtgatga
     3061 tcgtctactg gatatacaac tttgaatact tcctcaattt gtgccatttg gtctgctgat
     3121 agtccctcat aaggaattat tacatgtata tcgatcttta atgcattttc tccacttcta
     3181 actattcttt gtattggtgt aactgagact gcaagatttt gtaaaagaga actcactcca
     3241 ccctgttcta aaacttgatc taaattatat gcagcagttt gttcccaatc acccacaaaa
     3301 tcttcaagtg taaacacaaa attttctcct ttactgcagg agctcttctt ttcctcctct
     3361 tacacacgca atttattgcg tttatcttta caattaaaag tatactcctt agagttaact
     3421 caaggtcaat aaaaattttt aatttctggg ctaaaatgaa aaagtaactt catgattgca
     3481 atagtaaatt catcttacaa gaagttagtc ttgcacataa atctagttga acggtaccat
     3541 aaaaataaga agcctgcaaa tgcaggcttc ttatttttat gctagcttga tgcagaattc
     3601 gcccttaagt ctaaaaatta ggggagatgt aaggatttgg gaaaaataga agatgttata
     3661 atcataaata tggtattcgt aggcttaaag tcaaaaagga ggtgaaatat aaatagattt
     3721 ttagctaaat taagtaagaa ataggaggag atttattgaa caaaaaatta gaaaaaccat
     3781 ttgtatataa gagagagtac gatttgactg gatatgatgt tgaaatttta caaaaatatg
     3841 agttagaaca agcaatatat gtttatgttg ggagtagttg tgcatataac atgagagcta
     3901 gaagtagtaa atggagatac catataagaa caaataataa gtctatatgt tgtaacatta
     3961 aaaattttat acataacttg gaattgtttt ataaaatgga attaaagttg tcagataata
     4021 ttattaatga taagctatac tatagcaata tagcagagtt tgaagaattt gaaacactag
     4081 aaaaagctag agaggtagaa agtactatta taagtcaata tcaattttta gattctataa
     4141 atcatatgtt aaaacaaaaa ataattttat tgagtaataa ggatagtgtg ttaaacataa
     4201 ctaaaaatgg aaatacaaat tatttgaaag taaaaaataa atacatagaa aaacataaga
     4261 acaagccaat aatgagatac catatcaact gtcaattcaa tacagatgga agtgtcaaaa
     4321 gtattacaca ggagtttgaa ccaatattgg aattaaacaa aaaaaatacc ctaagccgac
     4381 caagcagagt atttttaaaa taatatttta agataacaac aaaatgagat aatactacta
     4441 gacaatgaca actcaactac caattgagtt tatggagcta ccaactccaa tatcggtcta
     4501 actgattaag tatctgtagt tatataataa tattgctatc aattttagca tcttaacaat
     4561 attattatac atactaagct aaaattattc aatagttgta aaagttgatt agtcaataag
     4621 tatatattta atgtagtgtt atctcttaaa aaaactagat aaggagataa taaatatatg
     4681 gaacaattag attcaaaata taagttgaaa aaatttctaa tggcagtatt tagagatggt
     4741 ataggacaag gaaataatct tattgataat gaatatgtta gagtatttca aaataataaa
     4801 agtaatagta aacaattaga actcggagaa gaatttaaag aatatagtaa aacaactttt
     4861 tttaaaaata tagatgatat agtagaattt accttcgcaa aaaatattta ttatgaaaat
     4921 acatttttta acctatgtac aactgatgga aaagcaggaa ccaatgaaaa cttaataaat
     4981 agatatgcat taggatttga ttttgacaaa aaagaattag gacaaggttt taattataaa
     5041 gatataatta atttatttac taagatagga ttacattatc atatcctagt tgatagtgga
     5101 aatggattcc atgtttatgt gctaattaat aaaactaata acattaagtt agtatcagaa
     5161 gttacaaata cattaataaa taaattgggt gcagataaac aagcaaattt atctactcaa
     5221 gtattaagag taccttatac atataatatt aaaaatacta ctaaacaagt aaaaataata
     5281 caccaagaca aaaatatata tagatatgac atagaaaagt tagctaaaaa atattgcaaa
     5341 gatgtaaaaa cagtaggtaa tactaataca aaatatatat tagatagtaa gctaccaaat
     5401 tgtatagtag atattttaaa aaatggtagt aaagatggac ataaaaacct agatttgcaa
     5461 aaaatagttg tgactttaag attgaggaat aaaagtttaa gtcaagtaat atccgttgct
     5521 agagaatgga actatatatc acaaaatagt ctttcaaata gtgagctaga atatcaagtc
     5581 aagtatatgt atgagaagct taaaacggtt aattttggtt gtacaggttg tgagtttaat
     5641 agtgattgtt ggaataaaat agaatcagat tttatatata gtgatgaaga tactttgttc
     5701 aatatgccac ataagcactc aaaggatttg aaatataaga ataggaaagg ggttaaaata
     5761 atgactggta atcaattgtt tatctataat gtgttactta acaataaaga tagagaatta
     5821 aacatagacg atataatgga gctgataacc tataaacgta agaagaaagt taaaaacatt
     5881 gttatgagtg aaaagacatt aagagaaaca ttaaaagaac ttcaacataa tgattatatt
     5941 acaaaaacaa aaggtgttac aaagctagga ataaaagata catacaatgt aaaagaagtt
     6001 agatgtaata tagataaaca atatactatt agttactttg ttaccatggc agtaatttgg
     6061 ggaataattt caactgaaga attaagatta tatactcata tgagatataa gcaagattta
     6121 ttggtcaaag atgataaaat aaaaggaaat atattaagaa ttaatcaaga ggaattagca
     6181 aaagatttag gagtaacaca gcaaagaatt tcaaatatga tagaatcttt attagatact
     6241 aaaattttag atgtatggga aactaaaata aatgatagag gatttatgta ctatacatat
     6301 agattaaaca agtagatttt tgataggatt agaattgatt ttctagtcct atttttatgc
     6361 aaaaaaacta attaataaaa atttcttttg gtaaaataat tgtacgagaa ttgcaaaaaa
     6421 aaaatggcat caaagtattg aaattaagcc gttttaaaaa tttcttttgg taaaataatt
     6481 gtacatatat atgtagtata tatatatatg ttttttagag aatgtataac tagaatatag
     6541 agctagaata tagagaatgt ataactagaa tatagagcta gaatatagag aatgtataac
     6601 tagaatatag agctagaata tagagaatgt ataactagaa tatagagcta gaatatagag
     6661 aatgtataac tagaatatag agctagaata tagagaatgt ataactagaa tatagagcta
     6721 gaatatagag aatgtataac tagaatatag agctagaatc ctaattagta ggtgcttttt
     6781 taaaacaagt taaaaatcaa aaatagtatt agtaagcatt ggaaatgcta gattctaaaa
     6841 tagaaaagta aaaaattggt gcactatcta aacttatcta tatcgctttt tccgtcgttt
     6901 ggttctctag ttacgataca ggggatatgc ttatattgag ttatagtact aatcagtgct
     6961 taatatagtt aataaaatta tagttaccat agtttagtaa ctatgatgta tgttagttag
     7021 aaacttgcat ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     7081 ccccgtagaa aagatcaaag gatcttc
//
