LOCUS       sequence-49899-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Hiraike H, Wada-Hiraike O, Nakagawa S, Koyama S, Miyamoto Y, Sone K,
            Tanikawa M, Tsuruga T, Nagasaka K, Matsumoto Y, Oda K, Shoji K, 
            Fukuhara H, Saji S, Nakagawa K, Kato S, Yano T, Taketani Y
  TITLE     Identification of DBC1 as a transcriptional repressor for BRCA1.
  JOURNAL   Br J Cancer. 2010 Mar 16;102(6):1061-7. Epub 2010 Feb 16.
  PUBMED    20160719
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Br J 
            Cancer."; date: "2010-03-16"; volume: "102(6)"; pages: "1061-7. Epub
            2010 Feb 16"
COMMENT     Sequence Label: pcDNA Myc DBC1
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..135
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccgcca ccatggaaca gaagctcata
      121 tctgaagagg acctcgaatt catgtcccag tttaagcgcc agcggatcaa cccgcttcca
      181 gggggacgca acttctcagg cacagcttca acatctcttc tgggtcctcc tcctggtttg
      241 ctcactcctc ctgtggccac agaactgtcc cagaatgcca ggcaccttca gggtggggag
      301 aaacagcggg tcttcactgg tattgttacc agcttgcatg actactttgg ggttgtggat
      361 gaagaggtct tttttcagct aagtgtggtg aagggccgtc tgccccagct gggtgagaag
      421 gtgctggtga aggctgcata caacccaggc caggcagtgc cctggaatgc tgtcaaggtg
      481 caaacgctct ccaaccagcc cctactgaag tccccagcac ctcctcttct gcatgtagca
      541 gccctgggcc agaagcaagg gatcctggga gctcagcctc agttgatctt ccagcctcac
      601 cggattcccc cactctttcc tcagaagcct ctgagtctct tccaaacatc ccacacactt
      661 cacctgagcc acctgaacag atttcctgcc cggggccctc atggacggtt ggatcagggc
      721 cgaagtgatg actatgactc caaga
//
