LOCUS       sequence-499031-       10736 bp    DNA     circular SYN 03-FEB-2026
DEFINITION  Optogenetic expression of Shh driven by five GAL4/GAVPO binding 
            sites in front of a synthetic YB-TATA minimal promoter in a PiggyBac
            integration plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10736)
  AUTHORS   Benzinger D, Briscoe J
  TITLE     Investigating morphogen and patterning dynamics with optogenetic 
            control of morphogen production.
  JOURNAL   Dev Cell. 2025 Aug 19:S1534-5807(25)00495-2. doi: 
            10.1016/j.devcel.2025.07.019.
  PUBMED    40848721
REFERENCE   2  (bases 1 to 10736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 3, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2025 Aug 19:S1534-5807(25)00495-2. doi: 
            10.1016/j.devcel.2025.07.019."
COMMENT     Sequence Label: pDBB56
FEATURES             Location/Qualifiers
     source          1..10736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(1902..2476,2477..2479,2480..2488)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2069..2086)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2296..2315
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2495..2872
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      2885..2894
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3630..3764
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3903..5079
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4132..5070
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5027..5047
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5089..6108
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     "
     primer_bind     complement(6180..6200)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    6242..6363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7126..7160
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7651..7667)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7651..7667)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7664..7686)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7675..7691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7699..7705,7706..7723,7724..7729))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7744..7765
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7882..7899)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8053..8641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8133..8152)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8812..9603,9604..9672))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9435..9454
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9673..9777)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9803..10258)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9940..9961)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10152..10171
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10385..10407
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10399..10416
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10400..10416
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   10546..10608
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aaaaacaaaa actcaaaatt tcttctataa agtaacaaaa cttttatgag ggacagcccc
       61 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc
      121 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg
      181 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
      241 tctttgagcc tgcagacacc tggggggata cggggaaaag gcctccacgg ccactagtaa
      301 acgcggccac tagtaaacgg agtttctaga cggagtactg tcctccgagc ggagtactgt
      361 cctccgactc gagcggagta ctgtcctccg atcggagtac tgtcctccgc gaattccgga
      421 gtactgtcct ccgaagacgc tagcgggcga tccatatgct ctagagggta tataatgggg
      481 gccactagtc tactaccaga aagcttggca ttccggtact gggtaaagcc gaattcctag
      541 cgccgccacc accatgctgc tgctgctggc cagatgtttt ctggtgatcc ttgcttcctc
      601 gctgctggtg tgccccgggc tggcctgtgg gcccggcagg gggtttggaa agaggcggca
      661 ccccaaaaag ctgacccctt tagcctacaa gcagtttatt cccaacgtag ccgagaagac
      721 cctaggggcc agcggcagat atgaagggaa gatcacaaga aactccgaac gatttaagga
      781 actcaccccc aattacaacc ccgacatcat atttaaggat gaggaaaaca cgggagcaga
      841 ccggctgatg actcagaggt gcaaagacaa gttaaatgcc ttggccatct ctgtgatgaa
      901 ccagtggcct ggagtgaagc tgcgagtgac cgagggctgg gatgaggacg gccatcattc
      961 agaggagtct ctacactatg agggtcgagc agtggacatc accacgtccg accgggaccg
     1021 cagcaagtac ggcatgctgg ctcgcctggc tgtggaagca ggtttcgact gggtctacta
     1081 tgaatccaaa gctcacatcc actgttctgt gaaagcagag aactccgtgg cggccaaatc
     1141 cggcggctgt ttcccgggat ccgccaccgt gcacctggag cagggcggca ccaagctggt
     1201 gaaggactta cgtcccggag accgcgtgct ggcggctgac gaccagggcc ggctgctgta
     1261 cagcgacttc ctcaccttcc tggaccgcga cgaaggcgcc aagaaggtct tctacgtgat
     1321 cgagaccctg gagccgcgcg agcgcctgct gctcaccgcc gcgcacctgc tcttcgtggc
     1381 gccgcacaac gactcggggc ccacgcccgg gccaagcgcg ctctttgcca gccgcgtgcg
     1441 ccccgggcag cgcgtgtacg tggtggctga acgcggcggg gaccgccggc tgctgcccgc
     1501 cgcggtgcac agcgtgacgc tgcgagagga ggaggcgggc gcgtacgcgc cgctcacggc
     1561 gcacggcacc attctcatca accgggtgct cgcctcgtgc tacgctgtca tcgaggagca
     1621 cagctgggca caccgggcct tcgcgccttt ccgcctggcg cacgcgctgc tggccgcgct
     1681 ggcacccgcc cgcacggacg gcgggggcgg gggcagcatc cctgcagcgc aatctgcaac
     1741 ggaagcgagg ggcgcggagc cgactgcggg catccactgg tactcgcagc tgctctacca
     1801 cattggcacc tggctgttgg acagcgagac catgcatccc ttgggaatgg cggtcaagtc
     1861 cagctgactt gatatcgaat tcctgcagcc cgggggatcc gcccctctcc ctcccccccc
     1921 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     1981 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     2041 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     2101 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     2161 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     2221 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     2281 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     2341 aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt tacatgtgtt
     2401 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     2461 aaaacacgat gataatatgg ccacaaccat ggttatgcca gagccagcga agtctgctcc
     2521 cgccccgaaa aagggctcca agaaggcggt gactaaggcg cagaagaaag gcggcaagaa
     2581 gcgcaagcgc agccgcaagg agagctattc catctatgtg tacaaggttc tgaagcaggt
     2641 ccaccctgac accggcattt cgtccaaggc catgggcatc atgaattcgt ttgtgaacga
     2701 cattttcgag cgcatcgcag gtgaggcttc ccgcctggcg cattacaaca agcgctcgac
     2761 catcacctcc agggagatcc agacggccgt gcgcctgctg ctgcctgggg agttggccaa
     2821 gcacgccgtg tccgagggta ctaaggccat caccaagtac accagcgcta aggatccacc
     2881 ggtcgccacc atggtatcaa aaggcgaggc ggtcattaaa gagttcatga ggtttaaagt
     2941 ccatatggaa ggaagcatga atggtcacga gtttgagata gagggagagg gtgagggaag
     3001 gccgtacgaa gggacgcaaa ctgcgaagtt gaaggtaacg aaaggcggcc ctctgccatt
     3061 ctcttgggac atcctttcac cgcaatttat gtacggctct agggccttca caaaacaccc
     3121 cgccgatatt ccggattact ataaacaaag ttttcctgag ggcttcaagt gggaaagagt
     3181 aatgaacttc gaggatgggg gagctgtgac ggttactcaa gacactagcc tggaggacgg
     3241 tactcttata tacaaggtaa aactaagagg gacgaatttt ccgccagacg gccccgtgat
     3301 gcaaaaaaaa actatggggt gggaagcatc caccgagagg ctgtaccccg aagacggggt
     3361 cctgaagggt gatattaaaa tggcattaag attaaaagat ggcgggagat acttagctga
     3421 cttcaagact acatataaag ccaaaaaacc tgtacagatg cctggagctt acaatgtgga
     3481 tcgtaatctg gatattacat cccacaacga agattatacc gtggtggagc agtacgagcg
     3541 ttcagagggg cgtcactcca ccgggggtat ggatgaatta tacaagtaag atttcgagcc
     3601 tctagaacta tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt
     3661 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     3721 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3781 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaattc gctgggatcc
     3841 gatttaaatt cgaattcggg cccgcggccg cactcaattc tctagaaata ttctcgaggt
     3901 ttctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3961 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4021 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4081 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     4141 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     4201 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     4261 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     4321 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     4381 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     4441 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     4501 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     4561 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     4621 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     4681 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     4741 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     4801 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     4861 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     4921 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4981 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     5041 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gcgccaccat gaaaaagcct
     5101 gaactcaccg ctacctctgt cgagaagttt ctgatcgaaa agttcgacag cgtgtccgac
     5161 ctgatgcagc tctccgaggg cgaagaatct cgggctttca gcttcgatgt gggagggcgt
     5221 ggatatgtcc tgcgggtgaa tagctgcgcc gatggtttct acaaagatcg ctatgtttat
     5281 cggcactttg catccgccgc tctccctatt cccgaagtgc ttgacattgg ggagttcagc
     5341 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcaccttgca agacctgcct
     5401 gaaaccgaac tgcccgctgt tctccagccc gtcgccgagg ccatggatgc catcgctgcc
     5461 gccgatctta gccagaccag cgggttcggc ccattcggac ctcaaggaat cggtcaatac
     5521 actacatggc gcgatttcat ctgcgctatt gctgatcccc atgtgtatca ctggcaaact
     5581 gtgatggacg acaccgtcag tgcctccgtc gcccaggctc tcgatgagct gatgctttgg
     5641 gccgaggact gccccgaagt ccggcacctc gtgcacgccg atttcggctc caacaatgtc
     5701 ctgaccgaca atggccgcat aacagccgtc attgactgga gcgaggccat gttcggggat
     5761 tcccaatacg aggtcgccaa catcttcttc tggaggccct ggttggcttg tatggagcag
     5821 cagacccgct acttcgagcg gaggcatccc gagcttgcag gatctcctcg gctccgggct
     5881 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     5941 gatgcagctt gggctcaggg tcgctgcgac gcaatcgtcc ggtccggagc cgggactgtc
     6001 gggcgtacac aaatcgcccg cagaagcgct gccgtctgga ccgatggctg tgtggaagtg
     6061 ctcgccgata gtggaaacag acgccccagc actcgtccta gggcaaagga tctgcagtaa
     6121 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6181 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgcgttaact
     6241 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6361 ttatcatgtc tggaattgac tcaaatgatg tcaattagtc tatcagaagc tcatctggtc
     6421 tcccttccgg gggacaagac atccctgttt aatatttaaa cagcagtgtt cccaaactgg
     6481 gttcttatat cccttgctct ggtcaaccag gttgcagggt ttcctgtcct cacaggaacg
     6541 aagtccctaa agaaacagtg gcagccaggt ttagccccgg aattgactgg attccttttt
     6601 tagggcccat tggtatggct ttttccccgt atccccccag gtgtctgcag gctcaaagag
     6661 cagcgagaag cgttcagagg aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc
     6721 ccgcacgctg ccggctcggg gatgcggggg gagcgccgga ccggagcgga gccccgggcg
     6781 gctcgctgct gccccctagc gggggaggga cgtaattaca tccctggggg ctttgggggg
     6841 gggctgtccc tgatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     6901 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     6961 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     7021 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     7081 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     7141 cgcagactat ctttctaggg ttaatctagc tgcatcagga tcatatcgtc gggtcttttt
     7201 tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa gaagcccgtg
     7261 ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac tgctgctgca
     7321 ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg gccatgcacg
     7381 cctttaacgg tgaactgttc gttcaggcca cctgggatac cagttcgtcg cggcttttcc
     7441 ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat accggcgaca
     7501 gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg ggatattacg
     7561 tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata ccccgtgagt
     7621 tacccggcgg gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7681 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7741 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7801 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7861 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8461 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     8521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8701 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8761 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8821 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8881 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8941 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     9001 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     9061 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     9121 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     9181 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     9241 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9301 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9361 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9421 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9481 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9541 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9601 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9661 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9721 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9781 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     9841 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     9901 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     9961 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
    10021 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
    10081 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
    10141 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
    10201 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
    10261 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
    10321 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
    10381 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcctcgtt cattcacgtt
    10441 tttgaacccg tggaggacgg gcagactcgc ggtgcaaatg tgttttacag cgtgatggag
    10501 cagatgaaga tgctcgacac gctgcagaac acgcagctag attaacccta gaaagataat
    10561 catattgtga cgtacgttaa agataatcat gtgtaaaatt gacgcatgtg ttttatcggt
    10621 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
    10681 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaa
//
