LOCUS       sequence-49915-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H,
            Chen-Tsai Y, Luo L
  TITLE     Extensions of MADM (Mosaic Analysis with Double Markers) in Mice.
  JOURNAL   PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27.
  PUBMED    22479386
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(3)"; pages: "e33332. Epub 2012 Mar 27"
COMMENT     Sequence Label: pMADM-alpha
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..106)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(281..300)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     550..568
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    818..851
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ccggtgctgc cggtgccatg ccccaggaac aggtggtggc ggccctcgga gcgctcgtac
       61 tgttccacga tggtgtagtc ctcgttgtgg gaggtgatgt ccagcttggt gtccacgtag
      121 tagtagccgg gcagttgcac gggcttcttg gccatgtaga tggtcttgaa ctccaccagg
      181 tagtggccgc cgtccttcag cttcagggcc tggtggatct cgcccttcag cacgccgtcg
      241 cgggggtaca ggcgctcggt ggaggcctcc cagcccatgg tcttcttctg cattacgggg
      301 ccgtcggggg ggaagttggt gccgcgcatc ttcaccttgt agatcagcgt gccgtcctgc
      361 agggaggagt cctgggtcac ggtcaccaga ccgccgtcct cgaagttcat cacgcgctcc
      421 cacttgaagc cctcggggaa ggacagcttc ttgtaatcgg ggatgtcggc ggggtgcttc
      481 acgtacgcct tggagccgta catgaactgg ggggacagga tgtcccaggc gaagggcagg
      541 gggccgccct tggtcacctt cagcttggcg gtctgggtgc cctcgtaggg gcggccctcg
      601 ccctcgccct cgatctcgaa ctcgtggccg ttcatggagc cctccatgcg caccttgaag
      661 cgcatgaact ctttgatgac ctcctcgccc ttactcacct aagggtggga aaatagacca
      721 ataggcagag agagtcagtg cctatcagaa acccaagagt cttctctgtc tccacatgcc
      781 cagtttctat tggtctcctt aaacctgtct tgtaaccata acttcgtata atgtatgcta
      841 tacgaagtta ta
//
