LOCUS       sequence-499515-       11388 bp    DNA     circular SYN 10-FEB-2026
DEFINITION  BCTV vector containing the GUS:NPTII repari template. Lacks the BCTV
            virion sense ORFs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Neubauer M, Vollen K, Zhao C, Ascencio-Ibanez JT, Hanley-Bowdoin L, 
            Stepanova AN, Alonso JM
  TITLE     Development of Modular Geminivirus-Based Vectors for High Cargo 
            Expression and Gene Targeting in Plants.
  JOURNAL   Plant Biotechnol J. 2025 Sep 21. doi: 10.1111/pbi.70320.
  PUBMED    40976866
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 10, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Sep 21. doi: 10.1111/pbi.70320."
COMMENT     Sequence Label: BCTV-Delta-VS-RT-pDGB3alpha2
FEATURES             Location/Qualifiers
     source          1..11388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..37
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1338..1967
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2396..3469
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3535..3729
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3965..3987)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4073..4213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4128..4147
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4228..4245)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4399..4987)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4479..4498)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5075..5890)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5075..5890)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5798..5817
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     misc_feature    6323..6347
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8239..9030
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8290..8309)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8900..8919
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9057..9309
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(9333..9352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9334..9352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(10492..10503)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tgagacgaag cttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
       61 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
      121 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
      181 tagtgcagtc ggcttctgac gttcagtgca gccgtcatct gaaaacgaca tgtcgcacaa
      241 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
      301 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
      361 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
      421 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
      481 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
      541 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
      601 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
      661 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
      721 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
      781 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
      841 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
      901 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
      961 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     1021 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     1081 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacctc
     1141 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     1201 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     1261 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     1321 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     1381 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     1441 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     1501 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     1561 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     1621 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     1681 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     1741 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     1801 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     1861 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     1921 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     1981 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     2041 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     2101 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     2161 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     2221 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     2281 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     2341 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     2401 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2461 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2521 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2581 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2641 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2701 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2761 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2821 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2881 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2941 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     3001 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     3061 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3121 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3181 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3241 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3301 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3361 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3421 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3481 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggactct ttcctgtgga
     3541 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3601 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3661 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3721 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3781 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3841 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3901 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3961 aaaacctctg acacatgcag ctcccggtga cggtcacagc ttgtctgtaa gcggatgccg
     4021 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     4081 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4141 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4201 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4261 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4321 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4381 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4441 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4501 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4561 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4621 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4681 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4741 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4801 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4861 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4921 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4981 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     5041 acgttaaggg attttggtca tgcattctag gtgattagaa aaactcatcg agcatcaaat
     5101 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     5161 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     5221 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     5281 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     5341 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     5401 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat
     5461 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     5521 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     5581 tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     5641 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     5701 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     5761 acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     5821 ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt tcccgttgaa
     5881 tatggctcat aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc
     5941 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
     6001 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
     6061 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
     6121 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     6181 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     6241 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     6301 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacata acaagcttcg
     6361 tctcagtcag gagccgttat aatattaccg gatggcccga aaaaaatggg caaccaatca
     6421 aaacgtgaca cgtggaaggg tactgtagaa tgatgtgacg tttttgagcg ggaaacttcc
     6481 tgaagaagat tcctttcaga taagatttgt tgactggtca atagaagggg acaactttat
     6541 taaagtaact ttactttatt aaaagtaaag ttagtgggcc ccacaggaaa cttgctcagc
     6601 aagttttaag tgggccccac aggaaacttg ctcagcaagt tttaactggg ccccgcagga
     6661 aacttgctca gcaagttttg cattatgtcg ttttatatac atttttttta catgtatatg
     6721 tacttataac catggcgtgc ctgcagacta gtatttaaat tagggataac agggtaatag
     6781 cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac
     6841 accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt
     6901 aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt
     6961 gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg
     7021 aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa
     7081 agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag
     7141 ggcgaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa
     7201 gatgcggact tgcgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta
     7261 atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg
     7321 ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt
     7381 aacctctctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa
     7441 gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg
     7501 cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt
     7561 ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg
     7621 acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc
     7681 gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat
     7741 ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat
     7801 cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac
     7861 accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt
     7921 gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg
     7981 caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg
     8041 aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg
     8101 cagcagggag gcaaacaacg cagggaggca aacaatgata tcacaactct cctgacgcgt
     8161 catcgtcggc tacagcctcg ggaattgcta cctagctcga gcaagatcca aggagatata
     8221 acaatggctt cctcctggat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     8281 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     8341 gtgttccggc tgtcagcgca gggtagaccg gttctttttg tcaagaccga cctgtccggt
     8401 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgta
     8461 ccttgcgctg ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     8521 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     8581 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     8641 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     8701 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     8761 gcgagaatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     8821 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     8881 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     8941 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     9001 ttctatcgcc ttcttgacga gttcttctga taaccgcgga gagctcgaat ttccccgatc
     9061 gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga
     9121 ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga
     9181 cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga
     9241 tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt
     9301 tactagatcg tgggcccgtc cagttccatt cgccctatag tgagtcgtat taaacctgca
     9361 ttaatgaatc ggccaacgcg cggggtaggg ataacagggt aatcccggga gagggtataa
     9421 tatttattat taataatgaa attagtacaa gttcattgca acactctggt tctgaattac
     9481 acttgataca aaattaaatt cttcatacag gacatgattt aaacctctaa ttacattatt
     9541 aatacaaatt aaacctagat tatccaagta tatctctagt ctctttttca agaccttcaa
     9601 gaaacggggt ccagtcaata tcctgttcga tccggttatc gttagctcta tccagcactt
     9661 gtgaagattc aacgctctcc ggaggttgta gttgaatcgg atttgtatct tcagttgata
     9721 gattggcccg tatctctcga accataggag tttcatatac agtggattcg gtacactctc
     9781 gaattccacg gaatttgttg cctgatggag agtaataggt tctcctgtgc gaaaatcctc
     9841 tattacactc atggtgtatt gtgaagtggc agttacaggg tagagtcacc ttgcgaggat
     9901 gcttctgctt ggttattaaa gagcggacct tggagtttgc agaaggttga attatgtaat
     9961 gtccaggact taagggcttc attttctggt ttttcaagaa attgttggta tgagctctca
    10021 ggtcctggat tgcataatat aatgcagggg ataccacctt taatgacacg tggttttcca
    10081 tactttaagt ttgtctgcca ctccttttgt gctccaataa ggtgcttcca gtgtttcatc
    10141 tttaagtaag tgggatctac gtcatcaatg acgttgtatt ccacttcatc ataatatgtt
    10201 ctaaggctaa aatctaaatg ccctgtgatg taattgtggg cccctaaaga tctagcccac
    10261 atagtcttcc ctgttcttga atcaccctct actatgatac tattatatct taaaggccgc
    10321 gcagcggcat caaccccaaa atatgcatca gcccattctt gcatttcctc aggaacgttt
    10381 gtgaatgagg acagaggaaa tagtggagtc catggatcag gtgccctctg aaatatcttt
    10441 tgagcattgt tcaaaagatt gtgatgttga aggaagtaat cctttggttg ttcttccttc
    10501 aatatttgaa gtgcttgttc aagagaagtt gcgtttaacg ccttggcata tgagtcgtta
    10561 gctgtctgtt gacctcctct agcacttcta ccgtcgatct ggaattcccc ccattcaatt
    10621 gtgtctccgt ccttgtcgac gtaggacttg acgtcggagc tggatttagc tccctgaatg
    10681 ttcggatgga aatgtgctga cctggttggg gataccaggt cgaataatct gatatttgtg
    10741 atctgaactt ttccttcgag ttgaagcagg acgtggagat gaggttgccc atcttcgtgt
    10801 agctctctgg caattttaat atatttttta ttagatgaaa gttgtattcc ttgaatttgc
    10861 tcaagagctt cttcttttga aagagaacac tgaggatatg taagaaatat gttttttgct
    10921 tgaatacgaa atcttttagt aggaggcatt ggactccgat gacgtggctt agcatattaa
    10981 catatctatt ggagtattgg agtactatat atagtagtac aactctcata agggccatcc
    11041 gttataatat taccggatgg cccgaaaaaa atgggcaacc aatcaaaacg tgacacgtgg
    11101 aagggtactg tagaatgatg tgacgttttt gagcgggaaa cttcctgaag aagattcctt
    11161 tcagataaga tttgttgact ggtcaataga aggggacaac tttattaaag taactttact
    11221 ttattaaaag taaagttagt gggccccaca ggaaacttgc tcagcaagtt ttaagtgggc
    11281 cccacaggaa acttgctcag caagttttaa ctgggccccg caggaaactt gctcagcaag
    11341 ttttgcatta tgtcgtttta tatacgttat ttttacatgt atatcgct
//
