LOCUS       sequence-499903-        5088 bp    DNA     circular SYN 11-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Engler C, Youles M, Gruetzner R, Ehnert TM, Werner S, Jones JD, 
            Patron NJ, Marillonnet S
  TITLE     A golden gate modular cloning toolbox for plants.
  JOURNAL   ACS Synth Biol. 2014 Nov 21;3(11):839-43. doi: 10.1021/sb4001504. 
            Epub 2014 Feb 20.
  PUBMED    24933124
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 11, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4001504";
            journalName: "ACS Synth Biol"; date: "2014-11-21- 21"; volume: "3"; 
            issue: "11"; pages: "839-43"
COMMENT     Sequence Label: pICH41551
FEATURES             Location/Qualifiers
     source          1..5088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             436..1227
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1398..1986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1887..1906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2140..2157
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
      361 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
      421 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
      481 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
      541 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
      601 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
      661 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
      721 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
      781 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
      841 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
      901 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
      961 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1021 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1081 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1141 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1201 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1261 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1321 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2161 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatca
     2221 ctctgtggtc tcaggaggtg ttcatctgtt ggacaagcta ataatatcta tttgttgtct
     2281 gaatcacaat tttcccatga taaattaaag ccaaaattcg gcttctcaca acctgataga
     2341 atctaatttt atgactttta tcaaaaaaaa gaagaagata gaatctaatt ttatgacatt
     2401 taatggtagc cattccttaa aaattcccga ttcaactaat ccttggagtt cattttctca
     2461 taggtggatt tcatgctgga gttactgtaa atagggttgg atttctacat tcttgcgaga
     2521 gtgatctgca ttccagcccc ctctgaagca caggagtcag ctgcatttca aatgattacc
     2581 caattacaga ctatggcaga ccgcacccat tagatttaat gtggcaaaga aacatcaact
     2641 ccaatatttt tgaatcctat taatggctat cataccatag tactgtatta gtactgtcca
     2701 gaagcaaaaa ttgttgtttt agaatattac ttggttgcaa ccgtatcagt gctttgaaca
     2761 tttatcaagg ccaaatacat aatggtgcct tgaaatcaac tctagtttat tgaaatgatg
     2821 tctattgagc acctatatgt gttgccagta ttgcccatgc ttaacttagc attctgttaa
     2881 caagtgttat gctggatgtt cgaatcgaac atggagtttt catgttgctt agaactatat
     2941 gctttcagtg ttagcagtat cggaaggtaa atacagttta aacaccaaaa gtagagagaa
     3001 ataaggagga atttaatctt tggatttgat tcgtctttct tgtgggaatg gtgaactcgt
     3061 taaccagcta ggtgaaatga actcaatact tagttcaaaa ttgtttaagt tccatttttg
     3121 gttaagagaa ttgagttgtg ctctctagct tttgaagatg atttaggttc aaggactata
     3181 attttacacg atattagagc tagatccatt gataggccgc gtttcttaat gtttttggcc
     3241 ttgtaattct gttttcacat tgattgactg aatgagttgt gctctttcta tatgatctcg
     3301 gataattctt tttctcatga gctaacttaa gttaggatat gagctaactg ctctccaaca
     3361 ggcaacaacc catagaggaa ccaatcagtt ttttcttttt gtcctcctct tccttgcacg
     3421 agtggactta gtaaatagaa aggaaaataa caaaaatgta taagaaacaa gtcaatctct
     3481 tgggtccctt aataatcaaa gaaaaccaat gaatgaactc caagtgcaca aaactaagat
     3541 aaggcaaagg aaaataattt gtagtaagaa tgctatatat tgataatgtt ggtaataata
     3601 ttatctagaa gcaatccaca gaaggggtgc cacgtgtcaa cttgtaaatc acaaattgca
     3661 agtcatcact aatattatta tcaagggacc atgcaaagtg aaaataaata attcatacta
     3721 agtactgatg aatcccctaa tgattttggt aaaaatcatt aagttaaggt ggatacacat
     3781 cttgtcatat gatcaaatgg tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa
     3841 ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc
     3901 aacagcttaa cgttggcttg ccacgcatta cttgactgta aaactctcac tcttaccgaa
     3961 cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga
     4021 ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc
     4081 tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga tattcgtgag
     4141 caaaaacgac ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt
     4201 tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta
     4261 gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc
     4321 tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta
     4381 agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta
     4441 catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat
     4501 ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc
     4561 tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag
     4621 ccatgggttc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat
     4681 atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac
     4741 ggactaggcc tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta
     4801 atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt
     4861 tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc
     4921 ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga cttactcgtg
     4981 gctgcaaccc tactagctca aaatttattc acacatggtt acgctttggg gaaattatga
     5041 ggggatctct cagtactaag tagtgagagc aatacttgag accacgaa
//
