LOCUS       sequence-500484-        7562 bp    DNA     circular SYN 19-FEB-2026
DEFINITION  mammalian expression of FLAG-tagged human androgen receptor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Bai S, He B, Wilson EM
  TITLE     Melanoma antigen gene protein MAGE-11 regulates androgen receptor 
            function by modulating the interdomain interaction.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(4):1238-57.
  PUBMED    15684378
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 19, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(4)"; pages: "1238-57"
COMMENT     Sequence Label: pCMV-FLAG-hAR
FEATURES             Location/Qualifiers
     source          1..7562
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             631..654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3586..4208
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3785..3804)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(4232..4252)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4237..4566
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4417..4552
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4604..4623)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4605..4623)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4637..4653)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4637..4653)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4650..4672)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4661..4677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4685..4691,4692..4709,4710..4715))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4730..4751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4868..4885)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5039..5627)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5119..5138)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5798..6589,6590..6658))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6421..6440
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6659..6763)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6790..7245
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6877..6896)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7087..7108
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7371..7393
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7385..7402
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7386..7402
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tctagccaag ctcaaggatg gactacaaag acgatgacga taaagaagtg
      661 cagttagggc tgggaagggt ctaccctcgg ccgccgtcca agacctaccg aggagctttc
      721 cagaatctgt tccagagcgt gcgcgaagtg atccagaacc cgggccccag gcacccagag
      781 gccgcgagcg cagcacctcc cggcgccagt ttgctgctgc tgcagcagca gcagcagcag
      841 cagcagcagc agcagcagca gcagcagcag cagcagcagc agcagcagca gcagcaagag
      901 actagcccca ggcagcagca gcagcagcag ggtgaggatg gttctcccca agcccatcgt
      961 agaggcccca caggctacct ggtcctggat gaggaacagc aaccttcaca gccgcagtcg
     1021 gccctggagt gccaccccga gagaggttgc gtcccagagc ctggagccgc cgtggccgcc
     1081 agcaaggggc tgccgcagca gctgccagca cctccggacg aggatgactc agctgcccca
     1141 tccacgttgt ccctgctggg ccccactttc cccggcttaa gcagctgctc cgctgacctt
     1201 aaagacatcc tgagcgaggc cagcaccatg caactccttc agcaacagca gcaggaagca
     1261 gtatccgaag gcagcagcag cgggagagcg agggaggcct cgggggctcc cacttcctcc
     1321 aaggacaatt acttaggggg cacttcgacc atttctgaca acgccaagga gttgtgtaag
     1381 gcagtgtcgg tgtccatggg cctgggtgtg gaggcgttgg agcatctgag tccaggggaa
     1441 cagcttcggg gggattgcat gtacgcccca cttttgggag ttccacccgc tgtgcgtccc
     1501 actccttgtg ccccattggc cgaatgcaaa ggttctctgc tagacgacag cgcaggcaag
     1561 agcactgaag atactgctga gtattcccct ttcaagggag gttacaccaa agggctagaa
     1621 ggcgagagcc taggctgctc tggcagcgct gcagcaggga gctccgggac acttgaactg
     1681 ccgtctaccc tgtctctcta caagtccgga gcactggacg aggcagctgc gtaccagagt
     1741 cgcgactact acaactttcc actggctctg gccggaccgc cgccccctcc gccgcctccc
     1801 catccccacg ctcgcatcaa gctggagaac ccgctggact acggcagcgc ctgggcggct
     1861 gcggcggcgc agtgccgcta tggggacctg gcgagcctgc atggcgcggg tgcagcggga
     1921 cccggttctg ggtcaccctc agccgccgct tcctcatcct ggcacactct cttcacagcc
     1981 gaagaaggcc agttgtatgg accgtgtggt ggtggtgggg gtggtggcgg cggcggcggc
     2041 ggcggcggcg gcggcggcgg cggcggcggc ggcggcgagg cgggagctgt agccccctac
     2101 ggctacactc ggccccctca ggggctggcg ggccaggaaa gcgacttcac cgcacctgat
     2161 gtgtggtacc ctggcggcat ggtgagcaga gtgccctatc ccagtcccac ttgtgtcaaa
     2221 agcgaaatgg gcccctggat ggatagctac tccggacctt acggggacat gcgtttggag
     2281 actgccaggg accatgtttt gcccattgac tattactttc caccccagaa gacctgcctg
     2341 atctgtggag atgaagcttc tgggtgtcac tatggagctc tcacatgtgg aagctgcaag
     2401 gtcttcttca aaagagccgc tgaagggaaa cagaagtacc tgtgcgccag cagaaatgat
     2461 tgcactattg ataaattccg aaggaaaaat tgtccatctt gtcgtcttcg gaaatgttat
     2521 gaagcaggga tgactctggg agcccggaag ctgaagaaac ttggtaatct gaaactacag
     2581 gaggaaggag aggcttccag caccaccagc cccactgagg agacaaccca gaagctgaca
     2641 gtgtcacaca ttgaaggcta tgaatgtcag cccatctttc tgaatgtcct ggaagccatt
     2701 gagccaggtg tagtgtgtgc tggacacgac aacaaccagc ccgactcctt tgcagccttg
     2761 ctctctagcc tcaatgaact gggagagaga cagcttgtac acgtggtcaa gtgggccaag
     2821 gccttgcctg gcttccgcaa cttacacgtg gacgaccaga tggctgtcat tcagtactcc
     2881 tggatggggc tcatggtgtt tgccatgggc tggcgatcct tcaccaatgt caactccagg
     2941 atgctctact tcgcccctga tctggttttc aatgagtacc gcatgcacaa gtcccggatg
     3001 tacagccagt gtgtccgaat gaggcacctc tctcaagagt ttggatggct ccaaatcacc
     3061 ccccaggaat tcctgtgcat gaaagcactg ctactcttca gcattattcc agtggatggg
     3121 ctgaaaaatc aaaaattctt tgatgaactt cgaatgaact acatcaagga actcgatcgt
     3181 atcattgcat gcaaaagaaa aaatcccaca tcctgctcaa gacgcttcta ccagctcacc
     3241 aagctcctgg actccgtgca gcctattgcg agagagctgc atcagttcac ttttgacctg
     3301 ctaatcaagt cacacatggt gagcgtggac tttccggaaa tgatggcaga gatcatctct
     3361 gtgcaagtgc ccaagatcct ttctgggaaa gtcaagccca tctatttcca cacccagtga
     3421 agcattggaa accctatttc cccaccccag ctcatgcccc ctttcagatg tcttctgcct
     3481 gttataactc tgcactactc ctctgcagtg ccttggggaa tttcctctat tgatgtacag
     3541 tctgtcatga gcttgcatgc ctgcaggtcg actctagagg atcccgggtg gcatccctgt
     3601 gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc caccagcctt
     3661 gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta taatattatg
     3721 gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg tagggcctgc
     3781 ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac tgcaatctcc
     3841 gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg attccaggca
     3901 tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt caccatattg
     3961 gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc tcccaaattg
     4021 ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt aaaataacta
     4081 taccagcagg aggacgtcca gacacagcat aggctacctg gccatgccca accggtggga
     4141 catttgagtt gcttgcttgg cactgtcctc tcatgcgttg ggtccactca gtagatgcct
     4201 gttgaattgg gtacgcggcc agcttggctg tggaatgtgt gtcagttagg gtgtggaaag
     4261 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4321 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4381 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4441 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4501 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4561 tgcaaaaagc tcctcgagga actgaaaaac cagaaagtta attccctata gtgagtcgta
     4621 ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4681 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4741 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4801 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4861 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4921 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4981 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     5041 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     5101 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     5161 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     5221 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     5281 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     5341 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     5401 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     5461 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     5521 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     5581 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5641 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     5701 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     5761 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5821 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5881 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5941 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6001 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6061 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6121 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6181 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6241 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6301 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6361 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     6421 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     6481 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     6541 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     6601 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     6661 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     6721 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6781 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6841 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6901 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6961 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     7021 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     7081 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7141 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7201 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
     7261 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     7321 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     7381 acgttgtaaa acgacggcca gtgccaagct gatctataca ttgaatcaat attggcaatt
     7441 agccatatta gtcattggtt atatagcata aatcaatatt ggctattggc cattgcatac
     7501 gttgtatcta tatcataata tgtacattta tattggctca tgtccaatat gaccgccatg
     7561 tt
//
