LOCUS       sequence-500511-        2747 bp    DNA     circular SYN 19-FEB-2026
DEFINITION  Expresses frame-shifted cI under the control of PLux promoter; part 
            of BNeuAS2 and BNeuAS3; p15A origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S
  TITLE     Multicellular artificial neural network-type architectures 
            demonstrate computational problem solving.
  JOURNAL   Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 
            10.1038/s41589-024-01711-4. Epub 2024 Sep 16.
  PUBMED    39285005
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 19, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01711-4"; journalName: "Nat Chem Biol"; date: 
            "2024-11"; volume: "20"; issue: "11"; pages: "1524-1534"
COMMENT     Sequence Label: pK1_PLux_R1_CI*
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             71..82
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             94..801
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="TKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQSG
                     VGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYPV
                     FSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGM
                     LILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVG
                     KVIASQWPEETFG"
     terminator      813..899
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1009..1111
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1112..1771
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1178..1197)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1792..1886
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2000..2545
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2446..2465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaataa
       61 agaattcatt aaagaggaga aaggtaccat ggcacaaaaa agaaaccatt aacacaagag
      121 cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc
      181 ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta
      241 tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa
      301 gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg
      361 gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag
      421 gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta
      481 agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg
      541 accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct
      601 gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc
      661 ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac
      721 ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg
      781 cctgaagaga cgtttggctg atctagaggc atcaaataaa acgaaaggct cagtcgaaag
      841 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      901 cgccgcccta gacctaggaa caaatgcgag atcttgatgt tggcgtcttc gttgtcgact
      961 gttcgtcttc gtcccagtag acgtcgatat ctggcgaaaa tgagacgttg atcggcacgt
     1021 aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt
     1081 atcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac
     1141 cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca
     1201 atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa
     1261 aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca
     1321 tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc
     1381 ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca
     1441 cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa
     1501 cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg
     1561 ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt
     1621 tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca
     1681 ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca
     1741 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
     1801 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
     1861 gccgggcgtt ttttattggt gagaatccaa gcactagtaa caacttatat cgtatggggc
     1921 tgacttcagg tgctacattt gaagagataa attgcactga aatctagaaa tattttatct
     1981 gattaataag atgatcttct tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa
     2041 acgaaaaaac cgccttgcag ggcggttttt cgaaggttct ctgagctacc aactctttga
     2101 accgaggtaa ctggcttgga ggagcgcagt caccaaaact tgtcctttca gtttagcctt
     2161 aaccggcgca tgacttcaag actaactcct ctaaatcaat taccagtggc tgctgccagt
     2221 ggtgcttttg catgtctttc cgggttggac tcaagacgat agttaccgga taaggcgcag
     2281 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
     2341 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggaa tgacaccggt
     2401 aaaccgaaag gcaggaacag gagagcgcac gagggagccg ccagggggaa acgcctggta
     2461 tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt
     2521 gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta
     2581 agtatcttcc tggcatcttc cagacatgtt ctttcctgcg ttatcccctg attctgtgga
     2641 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cggttgtttt
     2701 ggagcacgga actgcagaat gaatagcttc tgccaggatc ctggtaa
//
