LOCUS       sequence-500567-        9684 bp    DNA     circular SYN 19-FEB-2026
DEFINITION  AVAST from E. piriflorinigrans with Avs1a replaced with Avs1a from 
            Citrobacter Avs1a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   Doudna J et al.
  TITLE     Recurrent acquisition of nuclease-protease pairs in antiviral 
            immunity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 19, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLG017_Avs1a_chimera
FEATURES             Location/Qualifiers
     source          1..9684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      6103..6150
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(6370..7029)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6944..6963
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(7030..7132)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(7524..7541)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7658..8203)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7738..7757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8428..8532
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acactgtgag atatgctgct actgaaactg aaataaggaa cgcaactgta ctcattgaat
       61 gcgcgggtta cactggttcc ggaaccctga tcgcagcaga caaggtcctt acggctgcac
      121 attgtgtagt atcggatgat cctgagacac caattacagt gacatttttt ggtgcggatg
      181 aagacgtctg tgtcaatgcg acaatttcag aaatagatac atcgtgcgat gcctgtctgc
      241 taacactttc tgactctgtc gacattccgc ctattacact tatgacacag ccggagcgag
      301 agggaagcca atggaaagcc tttggctatc cggcatcacg caatgggcca tcacattatc
      361 ttcatggcac tataagtcag attttaccaa ggcttttcca tggcgttgat atggatttgt
      421 cggtcagtgc cgattgtgtt ctggaagagt acagtggagt ttctggtgcc gccattctat
      481 cagaaaataa atgcattgcg atggtgcgca tcaggatgga tggtggacta ggtgcagtaa
      541 gtcttgataa gttaagcggt ttgctgattc gaaacggcct aatcccagat gacattgcat
      601 ccctgccaga ttcatcactg tcgggtgaag ttgtcctgaa ccgcacagaa tttcgcgaca
      661 actttgaatc gttcgtcctg gagcacaagg gacgtgcagt gcttttggaa ggtagtcccg
      721 gctctggtaa gactaccttc tgccgccatt atcagccccg tagtgagcaa ctcgcagtgg
      781 cgggtgtcta tgaatttaca ccggaagacg gtgctggtac gacattcaaa attcttcctg
      841 aggtatttgc cgattggctg cataaccagg tttctatact gctttcaggt aggcctgctc
      901 gcagggagga aacagaaaag atcaatctga cccaaaaggt gtctgacctt ctacatactt
      961 tctcagatta ctggaagcac aaaggaaaat atggcgtcat tttcattgat gctgtgaatg
     1021 aggcaagcga gtgcggggat gaggcagtat cgcgctttac agcattactg ccggtgacac
     1081 ttccggagaa cgtcaaactt gttttcaccg caccatcatt atcatcagct ggtaaggctt
     1141 tccggcactg gctcacacct caggattgta tcagcctaac gcttttaagc catagggagg
     1201 tgttacagct aacagctcga gagcttaaaa cttccgcccc ttctttgtca ctactcacac
     1261 gagttagtga tatagctcag ggccatccac tttatctccg atacattctt gggtatctga
     1321 aagcgaatcc ggatcaggtt aatctggaga tattcccggt tttcagtggc agcattgaaa
     1381 cctactacga aaggctctgg caggggctgg ttaaggatga gagcgctgta aatctgctcg
     1441 gtattctctc gcggatgcgc tggggcattg atatttcatc actgatccct gttctaacac
     1501 cgcaggaaca gacggtgttt gttccaaccc ttgaccgtat tcagcatctg cttcttaatg
     1561 ataaatcatc agcattgtgc caccaatcat ttgcggcgtt tatcaacagt aaaacggcgg
     1621 taattaactc gctgctgcac ggacgccttg ccgacttctg ccttaccagt ggagagagtt
     1681 atggcctgat taatcgcgct tatcacctgc tcctagcctc tcacgacaga catcctgaag
     1741 ccgcattggt gtgcacgcag gaatgggctg acgcctgtat cgtcaagggg gctcagccgg
     1801 atattctaat tcacgatatc cgtcagaccc tgaagaacac gcttattcgt gccgatgcag
     1861 tggcatcgat tcgtctgttg ctgcttttcc aacgcatgac cttcagacac cattttttgt
     1921 ttctgcagtc agcttatcac tcaggccttg ccctggctgc acttggcaga ccggatgagg
     1981 cccttgagca gctcatacca tctggaagcc tcgttgttga tgcagttgat gcaattgtca
     2041 gcgcacagac tctcgcgcgt atgggaaaca gtgaacacgc gctgaagcta ttggaaaagg
     2101 tgaagtcagc tgtcgaccaa gaatttgaac gcaatcccgt caatctatct gattttatcg
     2161 gcctttccct ggcttgggtg agagctgagc tgatggctgg ggtggttgat ggccacggac
     2221 gcacacgcga ggttgttgag tatttgtacg gttgtgggca agtcgttcgc gataattttg
     2281 aacaatcagc gcatagtaaa tcagcatata cacgcgcttt ttatcctctt caggcagaaa
     2341 tggaagccgt gaacatagcc tttaatgacc gctccgtatc tttacggacg gttaaagaaa
     2401 agtttggtag cttaccggaa aatattcttg atctgatgct cagttcagtt atgcgggcac
     2461 atgacatcat tctgcaacat cagttgccga tgccccagca tgctttgcaa cccgtttggt
     2521 acaatctgga cagattactt catactgata ttccgtattc gaacgaaatt cgttttaatt
     2581 cattaagtag ccttattttt ttcaatgcgc cttctgctct tattatcagg atggcggggg
     2641 tattttcttt cgaagtagta cccgaaataa cgttgctcaa tgaagaaaat gagatagcag
     2701 cagacagcat tgacgttagt gaacagggac aactctggct ggtgagcgcc taccttaatg
     2761 aaacgcaacc ctgtcccgat attaaacatc cgagtcaggg atgttctgaa tggctcaaga
     2821 cattgactga ggctattttt tggtacagcg ggcaggcgcg ccgggcagtt attgacggca
     2881 acgatgagaa aaaagaactg cttttagtca aggtgcagaa tgatattctc cctgctcttt
     2941 cgtactcgct ggaagagcgc atggcatggc cgaattcatg ggcaatgcct gaacagatta
     3001 tccccatgat ttacgaagag ttagtaaaca tgttcggcgc atgctggccc gataagatat
     3061 cagtgatcac tgatttcatt ctggctcata cgcctcagca atgtggactt tattccgagg
     3121 ggtacaggcg tttactgaac agagttattc agactcttct aaatgagcat cggtttttgg
     3181 ggcaatctga tacgacattt caactacttg agacgttgca tgcgtttgtt tctgctttta
     3241 ctgagaatcg gcaggagctg gttcctgaat tactgaatat tattccagct tatattagcc
     3301 ttgatgctcc tcagctggca caggacactt acactgagct tttaggtgtg tcgatgggcc
     3361 ctgactggta caaagaagac caatttgccc tcatgacaac tatgctgcgc gtgataccac
     3421 agcatacaga cacaaatact acactttcac aagttgcagg attccttgaa catgcttcgg
     3481 gtgaaatgac atttaggcgt tatgttaggc aggaaaaatc acagtttatt ggcgaactta
     3541 ttcgtcgtgg gaattatgca cacgggttta actattatcg tcagcagtcc tgcggatccc
     3601 atgaggaaat gctcacccaa cttagccacc cagctgcaga tagccctcat ccattgaaag
     3661 gcatgcggtt cccgggggga gcgctggatg aggaacatgc tgtagaatgc attgtcagtg
     3721 aactgcgaaa cagagtcgac tggcggcttc gctggggact tcttgaaata ttcagctttg
     3781 gcagtattgg taatcttgca gtgccctttg ctgaacttat caatgaattt tctgcagaca
     3841 ctgaagacct taatgaaata cccaaaaggt tgcacaacat tttacatggt gatgtgcctt
     3901 tctcagaaca cagaaatttt atcaaaaatt tcacagagca ccttgcagac aaccataagc
     3961 cactctttgc tgaatttatc agtttgctat ccgaagacac tagcgataac gacgttaagc
     4021 ctcccccctc tggtgatgct aaccagaagg gtactgatac ctcagatgat gtggcaatgc
     4081 agccaggact ttttgggaag cgttctgcga tcaatagggc tgaagcctgc atggaaaatg
     4141 cccgaaaagc cgcagcacgc agaaacacag ttcgtgcaag tgagttagcc gttgaaagcc
     4201 tgcatataat tcaggatggt gactggtcag tctggagaaa gaacaaccat ctggcggaac
     4261 ttacacggac gtacatattg gacaactctg cggatgcagg ttcggtcatt cgtgcttatg
     4321 cttcgcttgt agaaaaagaa cgttatgccc cggcatgggt aattgctagt catctcatcg
     4381 aaatagcagc cagtaaattc tctgatcaag aagcccaagc tattaaccag atcgtacttg
     4441 aacacaaccg ccacatgctt gggaataccg aagcggatgc tgcgcatttt tcttttctta
     4501 atgaacctga tacctcagat gcaggtgaag aaacactcta ttttctgttt tggctgctgg
     4561 aacacccact gaaattcaga cgcgaacggg ctctggaagt actgaagtgg cttgcatcag
     4621 acgatgataa gattctgggc caatgcgtga cggaggcact cgtttcagac attgcctcac
     4681 gagctgaagc actaatggca ttgacagact gggtgtcagc tagatctcct cagcgaatat
     4741 gggactttat agttaaagag cgcagccttt ttgaatggct tgaaggcact actgcactaa
     4801 gccaagtcca tctcctggag cgagtaacca gcagagcggg atttgtttta agaaatgaga
     4861 ttgccgcatt tgagcgaccc cgaaagcttt tactgacatc agaagcctct ggacaacgga
     4921 atattccaga aaatttacca acatgggtgc aatccttgtc gcagaccctt gccgtgatgg
     4981 aaaagcaggg aatagatatc ccagctttgc ttaccttact cgaaaaacgg gttttacagc
     5041 agagtggatt ggctgatatc acggtggctt ttgagctgga aaagttactt gcgcgtggtt
     5101 ttactgtgaa tagaacacca agtcaccatc gctgggagac gatggtgcga tttgcattaa
     5161 accagatcat acatgaggcg gccgcacagg atgaactgca aaacattgaa cccttgctac
     5221 gtgcctggaa ccccgcgtca gaggagtgtg ttgagccgtg ggaggtttgt aaccgggcaa
     5281 aacagattat ctgcgctgtt atggaaggta gacatcagca agcttcgggc atagaggatg
     5341 gctttttctt gcattatctt gatgaagtgg aggtttcccg agaaggtcaa acgcatctgg
     5401 tggaaatctc agcggtgtta acgacagctc ataatggtca tgagagcctt agaccaggtg
     5461 cagaaagcga atttaatgca acacagacac ctgatataga gcggacgctt agtgtgcacc
     5521 ttacatgcca gcgagtcaaa atgcagcctt tgctttttgg gggagctacg cctgccgcag
     5581 tgtcgaaaaa gtttatgcag atgactggaa cgttgccttc agactttatt cgcaggcaat
     5641 ggcgaagcgg gcgttctctt agtaaaaaca gatgggggga accaataagc agaggaagtc
     5701 tgttactcat gaaaagaaca actaccctcc ctccaggact gggcttagcg tggtatgtca
     5761 ctgtcgatgg gaagttgatg aatatatttt catatgcccc gaggaggaga taatgaaata
     5821 cagttcaatg gaaacgccaa aaacgcgaga ggaatttgag gctcgctgtt ttcacctgct
     5881 caatgcgatc aagttaggac ggtatcatgg cattccgggt gaaggtaaca aagagcaggt
     5941 tccttttctc cctaacggac gagttgatct ggcaaacatt gataccatga ctcgcctctc
     6001 gatgaactcg ttatatgatt tccactataa cagggataat tatccgcagt ttgatctctc
     6061 tgaaaatgac gagaatgaag aggctacgga ttgacgcaat aactagcata accccttggg
     6121 gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga gaagcacacg
     6181 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     6241 taacgaccct gccctgaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
     6301 tccgcttatt atcacttatt caggcgtagc accaggcgtt taagggcacc aataactgcc
     6361 ttaaaaaaat tacgccccgc cctgccactc atcgcaatac tgttgtaatt catttaacat
     6421 tctgccgaca tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag
     6481 caccttgtcg ccttgcgtat aatatttgcc catagtgaaa acgggggcga agaagttgtc
     6541 catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa
     6601 aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac
     6661 atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga
     6721 tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat
     6781 caccagctca ccgtctttca tcgccatacg gaactctgga tgagcattca tcaggcgggc
     6841 aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa
     6901 ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc
     6961 ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt
     7021 tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg
     7081 tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca
     7141 ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta
     7201 ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc
     7261 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     7321 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     7381 ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt
     7441 cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg
     7501 tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
     7561 cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata
     7621 cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg
     7681 acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa
     7741 gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt
     7801 ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct gacactcagt
     7861 tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac
     7921 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagca
     7981 ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt catgcgccgg
     8041 ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc agttacctcg
     8101 gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg cggttttttc
     8161 gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc atcttattaa
     8221 tcagataaaa tatttctaga tttcagtgca atttatctct tcaaatgtag cacctgaagt
     8281 cagccccata cgatataagt tgtaattctc atgttagtca tgccccgcgc ccaccggaag
     8341 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     8401 agctaactta cattaattgc gttgcgccgc ggaaccccta tttgtttatt tttctaaata
     8461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8521 aaaaggaaga gtttggttgc gataaaaatg tatcccgcaa gggatgggga tgctttgctc
     8581 attatctgtc atcaggaaaa atgcggattt ttcatcgacg gcggctatgc agaaacctac
     8641 aagcaataca tcctgcctga tctcagagca ttgagtctta acgggtaccg gttacgccta
     8701 gtcatggcaa cgcatattga cgctgatcac atcggcggtc tcattgactt ttttatcgac
     8761 aatggccagg cgtcacatcc gtctgttata ccggttgatg cggtatggca caatagtctc
     8821 agggcgatga caacatctca tgaaaatgca tcagatgcag aaaatagcaa aatcacggat
     8881 tttttaaaac ggcaatatca tctcgctgca aagtcaggca gacccactga aattagcgct
     8941 cgccagggaa gttcgctcgc agccacattg ctggccggcg gctatcactg gaatgaagga
     9001 actggatttc aaaagatttg ctccttaagg ccagctgtcc cgccgttaaa cgaaaacagt
     9061 tttcagatcc tgagcccctc agaggaacgg ctctctgcac tctgtttgtg gtggcgccgt
     9121 aaactggtat cactgggttt ttctggccag tattccgcaa acggggcatt tgatgatgca
     9181 tttgagttcc tttgcaaaga ggatgcttcg ccgccacctc ctcctcatat gataagcgct
     9241 aaaatccctc tgcttcagag ggattattta cccgacactt cgccaaccaa tggcagctcc
     9301 attgccttta gcctgacact tagcaacaag cgaatgctga tgctgggaga cgcatgggct
     9361 gaagaggtag tgacctcact caaagccaac ggtggtcttc agtatttcga catcattaaa
     9421 atctcgcatc atggtagtat aagaaacacc agcccagagc tgttgagcct cattgatgca
     9481 ccaatgtatc tgatctcaac tgacggaaaa aaacatgcca ggcatcctga cctggcggtg
     9541 ctgaaggcag ttgtggacag acccgcgacg tttacacgaa cattattttt taactatgcc
     9601 acccgcgcat ctgctttcat gaaaacgtat gtttcagcaa acggcgcacc attcagggtg
     9661 attgaaggag caacggattg gata
//
