LOCUS       sequence-500606-        3086 bp    DNA     circular SYN 26-MAR-2026
DEFINITION  mTagBFP2 expressed under PL LacO1 promoter; part of BNeu AS11*; 
            colE1 origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S
  TITLE     Multicellular artificial neural network-type architectures 
            demonstrate computational problem solving.
  JOURNAL   Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 
            10.1038/s41589-024-01711-4. Epub 2024 Sep 16.
  PUBMED    39285005
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01711-4"; journalName: "Nat Chem Biol"; date: 
            "2024-11"; volume: "20"; issue: "11"; pages: "1524-1534"
COMMENT     Sequence Label: pM2_PLLacO1_R1_mTagBFP2
FEATURES             Location/Qualifiers
     source          1..3086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    9..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    32..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             90..101
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             108..809
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     terminator      821..907
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1180..1768)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1260..1279)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1856..1950)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1980..2771,2772..2840))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2603..2622
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2841..2945)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3054..3072
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagaatt gtgagcggat aacaattgac attgtgagcg gataacaaga tactgagcac
       61 atcagcagga cgcactgacc gaattcatta aagaggagaa aggtaccatg agcgagctga
      121 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtggacaac catcacttca
      181 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
      241 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctactagc ttcctctacg
      301 gcagcaagac cttcatcaac cacacccagg gcatccccga cttcttcaag cagtccttcc
      361 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
      421 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
      481 acttcacatc caacggccct gtgatgcaga agaaaacact cggctgggag gccttcaccg
      541 agacgctgta ccccgctgac ggcggcctgg aaggcagaaa cgacatggcc ctgaagctcg
      601 tgggcgggag ccatctgatc gcaaacgcca agaccacata tagatccaag aaacccgcta
      661 agaacctcaa gatgcctggc gtctactatg tggactacag actggaaaga atcaaggagg
      721 ccaacaacga gacctacgtc gagcagcacg aggtggcagt ggccagatac tgcgacctcc
      781 ctagcaaact ggggcacaag cttaattaat ctagaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
      901 gacaaatccg ccgccctaga cctaggcgat gacggaaaaa gagatcgtct taatgtatag
      961 attttcgtgg attacgtcgc cagtcaggat cctggcagaa gctattcatt ctgcagttcc
     1021 gtgctccaaa acaaccgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1081 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1141 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1201 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     1261 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     1321 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     1381 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     1441 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     1501 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     1561 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     1621 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     1681 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     1741 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1801 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgactagtg cttggattct
     1861 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1921 aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg gtctgacagt
     1981 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2041 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2101 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     2161 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     2221 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     2281 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     2341 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     2401 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     2461 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     2521 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     2581 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     2641 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     2701 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     2761 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     2821 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     2881 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     2941 ccgcgcacat ttccccgaaa agtgccacct gacgtctact gggacgaaga cgaacagtcg
     3001 acaacgaaga cgccaacatc aagatcttta ttatcatgac attaacctat aaaaataggc
     3061 gtatcacgag gccctttcgt cttcac
//
