LOCUS       sequence-500610-        3144 bp    DNA     circular SYN 20-FEB-2026
DEFINITION  LuxR regulator protein expressed under BBa_J23102 promoter; part of 
            BNeu AS4, AS8, AS9 and AS14; colE1 origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S
  TITLE     Multicellular artificial neural network-type architectures 
            demonstrate computational problem solving.
  JOURNAL   Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 
            10.1038/s41589-024-01711-4. Epub 2024 Sep 16.
  PUBMED    39285005
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 20, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01711-4"; journalName: "Nat Chem Biol"; date: 
            "2024-11"; volume: "20"; issue: "11"; pages: "1524-1534"
COMMENT     Sequence Label: pM2_J23102_R1_LuxR
FEATURES             Location/Qualifiers
     source          1..3144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..41
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             51..62
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      879..965
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1238..1826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1318..1337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1914..2008)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2038..2829,2830..2898))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2661..2680
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2899..3003)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3112..3130
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctcgagttga cagctagctc agtcctaggt actgtgctag cgaattcatt aaagaggaga
       61 aaggtaccat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag
      121 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt
      181 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa
      241 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat
      301 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg
      361 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc
      421 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt
      481 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca
      541 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata
      601 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa
      661 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt
      721 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa
      781 ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt
      841 gtagatcact actagagcca ggcatcatct agaggcatca aataaaacga aaggctcagt
      901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
      961 caaatccgcc gccctagacc taggcgatga cggaaaaaga gatcgtctta atgtatagat
     1021 tttcgtggat tacgtcgcca gtcaggatcc tggcagaagc tattcattct gcagttccgt
     1081 gctccaaaac aaccgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gactagtgct tggattctca
     1921 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1981 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagtta
     2041 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2101 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2161 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2221 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2281 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2341 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2401 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2461 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2521 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2581 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2641 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2701 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     2761 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     2821 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     2881 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     2941 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3001 gcgcacattt ccccgaaaag tgccacctga cgtctactgg gacgaagacg aacagtcgac
     3061 aacgaagacg ccaacatcaa gatctttatt atcatgacat taacctataa aaataggcgt
     3121 atcacgaggc cctttcgtct tcac
//
