LOCUS       sequence-500612-        4086 bp    DNA     circular SYN 20-FEB-2026
DEFINITION  LuxR expressed under BBa_J23102; E2-Crimson under PCT synthetic 
            promoter; component of BNeuAS6; colE1 origin of replication.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Bonnerjee D, Chakraborty S, Mukherjee B, Basu R, Paul A, Bagh S
  TITLE     Multicellular artificial neural network-type architectures 
            demonstrate computational problem solving.
  JOURNAL   Nat Chem Biol. 2024 Nov;20(11):1524-1534. doi: 
            10.1038/s41589-024-01711-4. Epub 2024 Sep 16.
  PUBMED    39285005
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 20, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-024-01711-4"; journalName: "Nat Chem Biol"; date: 
            "2024-11"; volume: "20"; issue: "11"; pages: "1524-1534"
COMMENT     Sequence Label: pM2_PJ23102R1LuxR_PCTR1E2Crimson
FEATURES             Location/Qualifiers
     source          1..4086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             812..823
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(833..867)
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     primer_bind     912..930
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    994..1012
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1024..1042
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             1052..1063
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1070..1747
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     1468..1487
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      1759..1845
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2118..2706)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2198..2217)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2794..2888)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(2918..3709,3710..3778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3541..3560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3779..3883)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(3995..4081)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagatgat gcctggctct agtagtgatc tacactagca ctatcagtgt tattaatttt
       61 taaagtatgg gcaatcaatt gctcctgtta aaattgcttt agaaatactt tggcagcggt
      121 ttgttgtatt gagtttcatt tgcgcattgg ttaaatggaa agtgacagta cgctcactgc
      181 aacctaatat ttttgaaata tcccaagagc tttttccttc gcatgcccac gctaaacatt
      241 ctttttctct tttggttaaa tcgttgtttg atttattatt tgctatattt atttttcgat
      301 aattatcaac tagagaagga acaattaatg gtatgttcat acacgcatgt aaaaataaac
      361 tatctatata gttgtctttt tctgaatgtg caaaactaag cattccgaag ccattgttag
      421 ccgtatgaat agggaaacta aacccagtga taagacctga tgttttcgct tctttaatta
      481 catttggaga ttttttattt acagcattgt tttcaaatat attccaatta attggtgaat
      541 gattggagtt agaataatct actataggat catattttat taaattagcg tcatcataat
      601 attgcctcca ttttttaggg taattatcta ggattgaaat atcagattta accatagaat
      661 gaggataaat gatcgcgagt aaataatatt cacaatgtac cattttagtc atatcagata
      721 agcattgatt aatatcatta ttgcttctac aagctttaat tttattaatt attctgtatg
      781 tgtcgtcggc atttatgttt ttcatggtac ctttctcctc tttaatgaat tcgctagcac
      841 agtacctagg actgagctag ctgtcaactc gagttaccag gatctttatt atcatgacat
      901 taacctataa aaataggcgt atcacgaggc cctttcgtct tcacctcgag tacctctggc
      961 ggtgatagat tacctctggc ggtgatattg acatccctat cagtgataga gatactgagc
     1021 acatccctat cagtgataga gagaattcat taaagaggag aaaggtacca tggatagcac
     1081 tgagaacgtc atcaagccct tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     1141 ccacgagttc gagatcgagg gcgtgggcga gggcaagccc tacgagggca cccagaccgc
     1201 caagctgcaa gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     1261 gttcttctac ggctccaagg cgtacatcaa gcaccccgcc gacatccccg actacctcaa
     1321 gcagtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     1381 ggtgaccgtg acccaggact cctccctgca ggacggcacc ctcatctacc acgtgaagtt
     1441 catcggcgtg aacttcccct ccgacggccc cgtaatgcag aagaagactc tgggctggga
     1501 gccctccact gagcgcaact acccccgcga cggcgtgctg aagggcgaga accacatggc
     1561 gctgaagctg aagggcggcg gccactacct gtgtgagttc aagtccatct acatggccaa
     1621 gaagcccgtg aagctgcccg gctaccacta cgtggactac aagctcgaca tcacctccca
     1681 caacgaggac tacaccgtgg tggagcagta cgagcgcgcc gaggcccgcc accacctgtt
     1741 ccagtagtct agaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1801 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gccctagacc
     1861 taggcgatga cggaaaaaga gatcgtctta atgtatagat tttcgtggat tacgtcgcca
     1921 gtcaggatcc tggcagaagc tattcattct gcagttccgt gctccaaaac aaccgttcgg
     1981 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     2041 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     2101 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     2161 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     2221 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     2281 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2341 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2401 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2461 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2521 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     2581 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2641 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2701 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2761 cgttaaggga ttttggtcat gactagtgct tggattctca ccaataaaaa acgcccggcg
     2821 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     2881 caggagtcca agcgagctcg taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     3541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     3601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     3661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     3721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     3781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3901 tgccacctga cgtctactgg gacgaagacg aacagtcgac aacgaagacg ccaacatcaa
     3961 gatctcgcat ttgttcctag gtctagggcg gcggatttgt cctactcagg agagcgttca
     4021 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
     4081 gatgcc
//
