LOCUS       sequence-500768-        6350 bp    DNA     circular SYN 23-FEB-2026
DEFINITION  CanABC from K. aerogenes under control of pTet and the native 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Doudna J et al.
  TITLE     Recurrent acquisition of nuclease-protease pairs in antiviral 
            immunity
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTet_KaeCanABC
FEATURES             Location/Qualifiers
     source          1..6350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(7..633)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     538..557
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        649..704
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    685..703
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      4515..4586
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4602..4629
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(4657..4674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4791..5336)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4871..4890)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5450..5544)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(5565..6224)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6139..6158
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6225..6327)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
       61 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      121 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      181 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      241 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      301 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      361 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      421 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      481 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      541 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      601 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
      661 tcgttgatag agttatttta ccactcccta tcagtgatag agaaaagaat tcaaaagatc
      721 ttttaagaag gagatataca ttagatagat cgcactcgaa tctgacagtc tggtttgggc
      781 caggagcaga ccttcgatgt atctggaaac taggggacat tcagctgagc atagagattt
      841 aaaaatacac ttgctaagct caatgcattg aaataaagat agttgaggta aatcttcaga
      901 caatttaccg catgtaccaa aaatagccct accgaagata aacttgagga atatcagtta
      961 gcccggataa ctgatatcaa gtaaaatgcc aaaactcaag gcttaactga gccggatatc
     1021 aatcaaggag tggctcgtcg aatgactgca gttatagatg atagctcaaa ctcacccgga
     1081 acccatgcgc ttatcattgg tgtaagcgcg tatgaccatc tgatgggctc tagtgaaggg
     1141 gaatgcagtg agcaaggctc agattctggt ctgggacagc taagtgcggc cgcaagatct
     1201 gcttcccagt ttgcgacgtg gttaatgaaa gattatcgac gcaaggactt tccgcttcgg
     1261 agtttgcggg tacttttatc gccagcgact gaagacgaag tgggtgaaaa cctcaagggt
     1321 tatcttaaag aagactcaat cccaacgagg gaaaatgtat caaccgcatt tagaaaattt
     1381 aaacaggcag ccgcaagaaa tccggataat atcgcaatcg tttatgtggc aggacatgga
     1441 gttcaattta ctaatactgg agcagtactc ctattaaagg attttgccca tcctgatcat
     1501 gaggaagaac gatataaggg agctgtagat attagagcca tgcatgaaag catgacgtat
     1561 acaaacgggg cgcggcgtca gttttggttt gtggatatat gcaggcaaag agctccggat
     1621 gctaaagagt tcgaggaatt agctggtgta ttaaaattca acaaagttat caatacaggt
     1681 agttctggag cgaccatatg ctccccgcta tttctggcgg catcatcggg cgagcaagcg
     1741 tttgccaaac ctggaaagct aactcttttc tgtgaggcct tgatgtcagc ccttgttgaa
     1801 ggtcatgctg cttcctccga tggggagcgc agttcaccat ggctagtatc aactagtgga
     1861 ttacagatgt atcttagaga cactgtagaa ttgttagctg cctgtaatga tgaaaaacaa
     1921 ttaatcgaat gctcagggat ttttagcaat gaagtctttc atgaatgcga aaaagctccg
     1981 aatgtagatt taacaataca gatcaaagac ggcttgcctg gggcccacta cagtgcaaaa
     2041 ttgtctcatc tcggcagagt tctttatgat aatcatacaa catggccgct tcgcgacagc
     2101 atacctgcag ggatctataa gatagaagtt acaactctaa ccactaatga gaaagactca
     2161 gataatctca aactttcgcc tccaagtagg ataaaggaac ttccactatg aatgaggtca
     2221 cacctaaaaa cataaacctt agtattagag tgaagtctaa attcaatttc ggcggacgag
     2281 acacgaaaat cgacatccga gatagtgaac tcattcttgt tgaaaagctc actacaagca
     2341 gagagttcag tctgctcccc ggactgtatg aagttagcac atttctgggc gatggaaaga
     2401 gagcatcgca gattgttgaa gtattcgaat cacaattaac tgagataagt ttagagccag
     2461 acattgatta tcagcctgcg cctccaaaag atgacagtca gtttaagaaa atcgaggagt
     2521 tgaatcagga taatcgattt ggtttgacta ttcttgatga agatactgac tttactattt
     2581 atccggctgg aagctattgg atcgtgcaac acaaagacgc aatgcaaaag atgcctataa
     2641 taacactcaa aaaaaatggg tcgtatattc aagctagcct ccctattagc caaggccacc
     2701 gttattccgt tacttgtcat gtgaaacagg tcttaaattc gaagtatggc cacgtagaaa
     2761 taaacatctc acagtctaga gctatggctt ttggcttgga gtgtatgctc aaaagtgggc
     2821 aaattatgaa tgcagccgag gtggcttcta atgcactaga agccttgata gaaaaataca
     2881 cggaccctac aggagcaaca cttgcagcac tgattcttta caagacatgc cgacttgatg
     2941 agtcgagcaa aagacattta atcaatttgg taacccatca tgattggtta cctgatgcaa
     3001 aaataatatt agctttatac ttagcacgca ctggcaacct ggacgaatca atgcaacttg
     3061 cgtgtgaagc tagcaaaaaa cgtattcttt taactgagag cttttcatta ttattggatc
     3121 ttcttcgtta ttggccagaa acagaacacg atgagaaaag aagcgaagct ctcgcacgtt
     3181 tagcgcaaat cgcgccattc gtaatttgga agtctatgta tcttaccacc agtatgaccg
     3241 gagattaaat tgttctcaat acaaatgcta cctgcacgtg aaggcgatgc aattttggtg
     3301 cgctggggag aggaacactc tccttatcaa atgcttgtcg atatgggggt cgaggaaaca
     3361 gggaaagagt ttaatgataa aattagcggc ctgcccgata cgcagcggac ctttgaagca
     3421 gttgtagtta cacatatcga cgcagaccat attggaggat ttttgagttg ttttgttgac
     3481 tgcgatgagc cgtgcgccat agtgaaagat ttttggttta atggttacat gcatctggat
     3541 ggtcgcagaa gcagttcttc tttagaaggg ctaggtgcag ttcaagccga aaggctcact
     3601 acatggttga gcacacagcc ctggaatgat tgttatgacg gaggctcagt ctgtcgaagc
     3661 gagggagaac cgcttaaagt cattgaactc gcaggaggca tgaaggtaac tgtccttggg
     3721 cctacgatgc aaagacttga aaagcttaaa cctgaatggg ataaagaaat aaataacgct
     3781 ttacaaaaaa aacaaaaaaa actctcagta cctaacctgg aatccatggg agcaagttta
     3841 ccattggaga ttccagacgc tcagtccctc aaatttttag ctaagcaaag gcccttgcga
     3901 gacactaaag ctgcaaatgg gagcagtatt gtcttactac tagaatatga tggtaaaaaa
     3961 gttctactcg ccggcgatgc gttcgctgaa gacttggttg atgctatttt agttttatca
     4021 agtgaatttc ccctaaagct cgaagctttc aaagtccccc accactgtag tcgtggaaat
     4081 atctcaaagc aactaattga agctgttgac tgcacaaatt ggcttatatc gacagatggc
     4141 agcagacata agcaccccga cgatgaaggg atagcgtcta tcctgcttca caggacaacc
     4201 gattttgtaa atttggtttt caatgtaact agcgattaca attcaaaatg gtcgaacacc
     4261 gagtggaaac aatgcttcag atattcagca aactatggct ctaaagaaga tggttattta
     4321 ctgaatcttt gattttataa atttatctta tttaaggtct gcgagtgctt gtagaattaa
     4381 cttccgcaac tcgctcttag cagaccacac agcccttcaa gcggtccgct actgccagaa
     4441 gcggaatttg ttaactttgt tatatgctct ttaaaaaatt atggatccaa actcgagtaa
     4501 ggatctccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     4561 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     4621 ctttctgcgt ttatacctag ggatatattc cgcttcctcg ctcactgact cgctacgctc
     4681 ggtcgttcga ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga
     4741 tgccaggaag atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg
     4801 ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg
     4861 acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt
     4921 cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg
     4981 cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc
     5041 cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag
     5101 acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga
     5161 agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa
     5221 gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca
     5281 aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag
     5341 atcatcttat taatcagata aaatatttct agatttcagt gcaatttatc tcttcaaatg
     5401 tagcacctga agtcagcccc atacgatata agttgttact agtgcttgga ttctcaccaa
     5461 taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc cagatggagt tctgaggtca
     5521 ttactggatc tatcaacagg agtccaagcg agctcgatat caaattacgc cccgccctgc
     5581 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     5641 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     5701 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     5761 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     5821 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     5881 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     5941 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     6001 atacgaaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     6061 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     6121 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     6181 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     6241 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     6301 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccagatatc
//
