LOCUS       sequence-500804-        6675 bp    DNA     circular SYN 23-FEB-2026
DEFINITION  Expression of soma-targeted calcium lifetime sensor LifeCamp under 
            hSyn promoter in pAAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6675)
  AUTHORS   Lodder B, Raghubardayal M, Ganesh S, Cai X, Stern J, Sherman M, 
            Rosen P, Kamath T, Hartman I, Siegel M, Timmins J, Adan R, Andermann
            M, Sabatini BL
  TITLE     Rapid fluorescence lifetime sensor development of LifeCamp enables 
            transient and baseline absolute calcium measurements
  JOURNAL   bioRxiv 2025.12.23.696288
REFERENCE   2  (bases 1 to 6675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.12.23.696288"
COMMENT     Sequence Label: pAAV-hsyn-RiboL1-LifeCamp
FEATURES             Location/Qualifiers
     source          1..6675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        825..1272
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1301..1318
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1600..1621
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1727..1748)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1988..2007)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(4208..4228)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     repeat_region   4588..4728
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4588..4717
                     /label=AAV2 ITR
     rep_origin      4803..5258
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4890..4909)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5100..5121
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5275..5294)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5394..5416
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5454..5472)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5540..5644
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5645..5713,5714..6505)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5863..5882)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca agtgggtttt
      841 aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga cccactggac
      901 aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg gaggggaaac
      961 aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt gccttcgccc
     1021 ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac gtcactcgcc
     1081 ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc cgccggccca
     1141 gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca tctgcgctgc
     1201 ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt cgtgtcgtgc
     1261 ctgagagcgc agtcgagaag gtaccggatc cgccaccatg catcatcacc atcatcacac
     1321 gcgtcgcaag aagaccttca aggaggtggc caccgccgtg aagatcatcg gccgcctgat
     1381 gggcctgaag atcaacgtct atatcaaggc cgacaagcag aagaacggca tcaaggcgaa
     1441 cttccacatc cgccacaaca tcgaggacgg cggcgtgcag ctcgcctacc actaccagca
     1501 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcgtgga
     1561 gtccaaactt tcgaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1621 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     1681 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1741 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggtgagggcg atgccaccta
     1801 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1861 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1921 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac atccaggagc gcaccatctt
     1981 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     2041 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     2101 caagctggag tacaacctgc cggaccaact gactgaagag cagatcgcag aatacaaaga
     2161 ggctttctcc ctatttgaca aggacgggga tgggacaata acaaccaagg agctggggac
     2221 ggtgatgcgg tctctggggc agaaccccac agaagcagag ctgcaggaca tgatcaatga
     2281 agtagatgcc gacggtgacg gcacaatcga cttccctgag ttcctgacaa tgatggcaag
     2341 aaaaatgaaa tacagggaca cggaagaaga aattagagaa gcgttcggtg tgtttgataa
     2401 ggatggcaat ggctacatca gtgcagcaga gcttcgccac gtgatgacaa accttggaga
     2461 gaagttaaca gatgaagagg ttgatgaaat gatcagggaa gcagacatcg atggggatgg
     2521 tcaggtaaac tacgaagagt ttgtacaaat gatgacagcg aagggcggct ctggaggatc
     2581 tggtggatca gggggctctg gaggtagtgg gggaagcggg ggctccggag ggagcggtgg
     2641 ttccggagga agtggtggca gcggcgggtc cgggggatca ggtgggtcca tggtatccaa
     2701 aggggaagaa ctcttcacag gagttgtccc catacttgtc gagctcgatg gcgacgttaa
     2761 cggtcacaaa ttctctgttt ctggcgaagg cgaaggggat gcaacatatg gcaaacttac
     2821 tcttaaattt atctgtacca ccgggaaatt gcccgttcct tggcctacac ttgtcactac
     2881 tcttggatat ggagtgcagt gcttcagtcg gtatccagat catatgaagc agcatgactt
     2941 ctttaagagc gccatgcctg aaggttatgt gcaagaacga accatattct ttaaagacga
     3001 tggtaattat aagactcgag cagaggtcaa attcgagggc gacacccttg tcaatcgcat
     3061 agaattgaag gggatagatt ttaaagaaga cgggaacatt ttggggcata agcttgagta
     3121 taacatgaac agccataatg tttatataat ggcagataaa cagaagaacg gaatcaaagt
     3181 aaactacaag atcaggcaca atattgaaga cgggagtgtt cagcttgccg accactacca
     3241 acagaacact ccaatcgggg acggacccgt cttgctgcct gacaatcatt atctctcaac
     3301 acagagcgca ctctctaagg acccaaacga aaagagagat catatggttc tcttggaatt
     3361 tgttaccgca gccggtatta cattgggaat ggacgaactc tataaagggg gcagcggtgg
     3421 gtctggaggg actgggggta gcggtggtag cggtgggaca gggggaagcg gcggtagtgg
     3481 tggaacaagc agcaaagtct cacgcgacac cctgtacgag gcggtgcggg aagtcctgca
     3541 cgggaaccag cgcaagcgcc gcaagtttct ggagacggtg gagctgcaga tcagcctgaa
     3601 gaactacgac cctcagaagg acaaacgttt ctcgggcacc gtcaggctca agtccacccc
     3661 acgccccaag ttctcggtgt gcgttctggg ggaccagcag cactgtgatg aagccaaggc
     3721 cgtggatatc ccccacatgg acatcgaggc gctcaagaaa cttaacaaaa acaagaagtt
     3781 ggtcaagaag ctggctaaga agtacgatgc ctttttggcc tctgagtctc tgattaagca
     3841 gatcccacgt atcctgggcc caggcctaaa caaggctggc aagttcccct ccctgctgac
     3901 acacaatgaa aacatggtgg ccaaagtgga tgaggtgaaa tcgacaatca agttccagat
     3961 gaagaaggtg ctgtgtttgg ccgtcgctgt tggccacgtg aagatgaccg atgatgagct
     4021 agtctacaac attcatctgg ctgtcaattt cttggtgtcc ttgcttaaga aaaactggca
     4081 aaacgtgcgg gctctgtaca tcaagagcac catgggcaag ccccagcgtc tgtattagga
     4141 attccgctcg agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4201 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4261 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     4321 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4381 gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4441 ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4501 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4561 atgtgggagg ttttttaaag cggccgcagg aacccctagt gatggagttg gccactccct
     4621 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     4681 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
     4741 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
     4801 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4861 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4921 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4981 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     5041 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     5101 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
     5161 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
     5221 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
     5281 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     5341 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5401 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     5461 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5521 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5581 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5641 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5701 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5761 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5821 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5881 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5941 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6001 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6061 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6121 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6181 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6241 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6301 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6361 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6421 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6481 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6541 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6601 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6661 gatcaaagga tcttc
//
