LOCUS       sequence-500862-        7306 bp    DNA     circular SYN 23-FEB-2026
DEFINITION  pSC101-ts plasmid with salicylic acid inducible Lambda-RED operon 
            and constituitive recA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Hina-Nilesh Joshi S, Jenkins C, Ulaeto D, Gorochowski TE
  TITLE     SHARK: A Specialized Host for Assembling R6K Plasmids.
  JOURNAL   ACS Synth Biol. 2026 Jan 13. doi: 10.1021/acssynbio.5c00749.
  PUBMED    41529903
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2026 Jan 13. doi: 10.1021/acssynbio.5c00749."
COMMENT     Sequence Label: pREMORAv1
FEATURES             Location/Qualifiers
     source          1..7306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      416..638
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             686..1636
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     CDS             complement(join(1766..2557,2558..2626))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2389..2408
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2627..2731)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2979..3395
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             3401..4186
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             4183..4863
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGEDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     CDS             4976..6037
                     /codon_start=1
                     /gene="recA"
                     /product="DNA repair protein from E. coli"
                     /label=RecA
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTGS
                     LSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDPIY
                     ARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEIGDS
                     HMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKFYASV
                     RLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELVDLGVK
                     EKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNSTPDFSV
                     DDSEGVAETNEDF"
     primer_bind     complement(7284..7303)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttccgaag tggtcagact ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc
       61 agatagcacc acatagcaga cccgccataa aacgccctga gaagcccgtg acgggctttt
      121 cttgtattat gggtagtttc cttgcatgaa tccataaaag gcgcctgtag tgccatttac
      181 ccccattcac tgccagagcc gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac
      241 tcaggtgcct gatggtcgga gacaaaagga atattcagcg atttgcccga ttgcggccgc
      301 aaccgagctt gcgagggtgc tacttaagcc tttagggttt taaggtctgt tttgtagagg
      361 agcaaacagc gtttgcgaca tccttttgta atactgcgga actgactaaa gtagtgagtt
      421 atacacaggg ctgggatcta ttctttttat ctttttttat tctttcttta ttctataaat
      481 tataaccact tgaatataaa caaaaaaaac acacaaaggt ctagcggaat ttacagaggg
      541 tctagcagaa tttacaagtt ttccagcaaa ggtctagcag aatttacaga tacccacaac
      601 tcaaaggaaa aggactagta attatcattg actagcccat ctcaattggt atagtgatta
      661 aaatcaccta gaccaattga gatgtatgtc tgaattagtt gttttcaaag caaatgaact
      721 agcgattagt cgctatgact taacggagca tgaaaccaag ctaattttat gctgtgtggc
      781 actactcaac cccacgattg aaaaccctac aaggaaagaa cggacggtat cgttcactta
      841 taaccaatac gttcagatga tgaacatcag tagggaaaat gcttatggtg tattagctaa
      901 agcaaccaga gagctgatga cgagaactgt ggaaatcagg aatcctttgg ttaaaggctt
      961 tgagattttc cagtggacaa actatgccaa gttctcaagc gaaaaattag aattagtttt
     1021 tagtgaagag atattgcctt atcttttcca gttaaaaaaa ttcataaaat ataatctgga
     1081 acatgttaag tcttttgaaa acaaatactc tatgaggatt tatgagtggt tattaaaaga
     1141 actaacacaa aagaaaactc acaaggcaaa tatagagatt agccttgatg aatttaagtt
     1201 catgttaatg cttgaaaata actaccatga gtttaaaagg cttaaccaat gggttttgaa
     1261 accaataagt aaagatttaa acacttacag caatatgaaa ttggtggttg ataagcgagg
     1321 ccgcccgact gatacgttga ttttccaagt tgaactagat agacaaatgg atctcgtaac
     1381 cgaacttgag aacaaccaga taaaaatgaa tggtgacaaa ataccaacaa ccattacatc
     1441 agattcctac ctacataacg gactaagaaa aacactacac gatgctttaa ctgcaaaaat
     1501 tcagctcacc agttttgagg caaaattttt gagtgacatg caaagtaagt atgatctcaa
     1561 tggttcgttc tcatggctca cgcaaaaaca acgaaccaca ctagagaaca tactggctaa
     1621 atacggaagg atctgaggtt cttatggctc ttgtatctat cagtgaagca tcaagactaa
     1681 caaacagcaa cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     1741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     2401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     2461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     2521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     2581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     2641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     2701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg cggagccaat
     2761 tattgaaggc ctccctaacg gggggccttt ttttgtttct ggtctgccgc ttaacgatcg
     2821 ttggctgggg gcctcgcttg ggttattgct ggtgcccggc cgggcgcaat attcatgttg
     2881 atgatttatt atatatcgag tggtgtattt atttatattg tttgctccgt taccgttatt
     2941 aactacttca cacaggaaac agtcgacgct tataaaaaat ggatattaat actgaaactg
     3001 agatcaagca aaagcattca ctaaccccct ttcctgtttt cctaatcagc ccggcatttc
     3061 gcgggcgata ttttcacagc tatttcagga gttcagccat gaacgcttat tacattcagg
     3121 atcgtcttga ggctcagagc tgggcgcgtc actaccagca gctcgcccgt gaagagaaag
     3181 aggcagaact ggcagacgac atggaaaaag gcctgcccca gcacctgttt gaatcgctat
     3241 gcatcgatca tttgcaacgc cacggggcca gcaaaaaatc cattacccgt gcgtttgatg
     3301 acgatgttga gtttcaggag cgcatggcag aacacatccg gtacatggtt gaaaccattg
     3361 ctcaccacca ggttgatatt gattcagagg tataaaacga atgagtactg cactcgcaac
     3421 gctggctggg aagctggctg aacgtgtcgg catggattct gtcgacccac aggaactgat
     3481 caccactctt cgccagacgg catttaaagg tgatgccagc gatgcgcagt tcatcgcatt
     3541 actgatcgtt gccaaccagt acggccttaa tccgtggacg aaagaaattt acgcctttcc
     3601 tgataagcag aatggcatcg ttccggtggt gggcgttgat ggctggtccc gcatcatcaa
     3661 tgaaaaccag cagtttgatg gcatggactt tgagcaggac aatgaatcct gtacatgccg
     3721 gatttaccgc aaggaccgta atcatccgat ctgcgttacc gaatggatgg atgaatgccg
     3781 ccgcgaacca ttcaaaactc gcgaaggcag agaaatcacg gggccgtggc agtcgcatcc
     3841 caaacggatg ttacgtcata aagccatgat tcagtgtgcc cgtctggcct tcggatttgc
     3901 tggtatctat gacaaggatg aagccgagcg cattgtcgaa aatactgcat acactgcaga
     3961 acgtcagccg gaacgcgaca tcactccggt taacgatgaa acaatgcagg agattaacac
     4021 tctgctgatc gccctggata aaacatggga tgacgactta ttgccgctct gttcccagat
     4081 atttcgccgc gacattcgtg catcgtcaga actgacacag gccgaagcag taaaagctct
     4141 tggattcctg aaacagaaag ccgcagagca gaaggtggca gcatgacacc ggacattatc
     4201 ctgcagcgta ccgggatcga tgtgagagct gtcgaacagg gggaagatgc gtggcacaaa
     4261 ttacggctcg gcgtcatcac cgcttcagaa gttcacaacg tgatagcaaa accccgctcc
     4321 ggaaagaagt ggcctgacat gaaaatgtcc tacttccaca ccctgcttgc tgaggtttgc
     4381 accggtgtgg ctccggaagt taacgctaaa gcactggcct ggggaaaaca gtacgagaac
     4441 gacgccagaa ccctgtttga attcacttcc ggcgtgaatg ttactgaatc cccgatcatc
     4501 tatcgcgacg aaagtatgcg taccgcctgc tctcccgatg gtttatgcag tgacggcaac
     4561 ggccttgaac tgaaatgccc gtttacctcc cgggatttca tgaagttccg gctcggtggt
     4621 ttcgaggcca taaagtcagc ttacatggcc caggtgcagt acagcatgtg ggtgacgcga
     4681 aaaaatgcct ggtactttgc caactatgac ccgcgtatga agcgtgaagg cctgcattat
     4741 gtcgtgattg agcgggatga aaagtacatg gcgagttttg acgagatcgt gccggagttc
     4801 atcgaaaaaa tggacgaggc actggctgaa attggttttg tatttgggga gcaatggcga
     4861 tgacgcatcc tcacgataat atccgggtag gcgcaatcac tttcgtctct aatgtataat
     4921 tgcttcaaca gaacatattg actatccggt attacccggc atgacaggag taaaaatggc
     4981 tatcgacgaa aacaaacaga aagcgttggc ggcagcactg ggccagattg agaaacaatt
     5041 tggtaaaggc tccatcatgc gcctgggtga agaccgttcc atggatgtgg aaaccatctc
     5101 taccggttcg ctttcactgg atatcgcgct tggggcaggt ggtctgccga tgggccgtat
     5161 cgtcgaaatc tacggaccgg aatcttccgg taaaaccacg ctgacgctgc aggtgatcgc
     5221 cgcagcgcag cgtgaaggta aaacctgtgc gtttatcgat gctgaacacg cgctggaccc
     5281 aatctacgca cgtaaactgg gcgtcgatat cgacaacctg ctgtgctccc agccggacac
     5341 cggcgagcag gcactggaaa tctgtgacgc cctggcgcgt tctggcgcag tagacgttat
     5401 cgtcgttgac tccgtggcgg cactgacgcc gaaagcggaa atcgaaggcg aaatcggcga
     5461 ctctcacatg ggccttgcgg cacgtatgat gagccaggcg atgcgtaagc tggcgggtaa
     5521 cctgaagcag tccaacacgc tgctgatctt catcaaccag atccgtatga aaattggtgt
     5581 gatgttcggt aacccggaaa ccactaccgg tggtaacgcg ctgaaattct acgcctctgt
     5641 tcgtctcgac atccgtcgta tcggcgcggt gaaagagggc gaaaacgtgg tgggtagcga
     5701 aacccgcgtg aaagtggtga agaacaaaat cgctgcgccg tttaaacagg ctgaattcca
     5761 gatcctctac ggcgaaggta tcaacttcta cggcgaactg gttgacctgg gcgtaaaaga
     5821 gaagctgatc gagaaagcag gcgcgtggta cagctacaaa ggtgagaaga tcggtcaggg
     5881 taaagcgaat gcgactgcct ggctgaaaga taacccggaa accgcgaaag agatcgagaa
     5941 gaaagtacgt gagttgctgc tgagcaaccc gaactcaacg ccggatttct ctgtagatga
     6001 tagcgaaggc gtagcagaaa ctaacgaaga tttttaaagg tactcggtac caaattccag
     6061 aaaagaggcc tcccgaaagg ggggcctttt tcgttttggt ccaatggcgg cgcgccatcg
     6121 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcatgg
     6181 gggttgaata tggaactgcg tgacctggat ttaaacctgc tggtggtgtt caaccagttg
     6241 ctggtcgaca gacgcgtctc tgtcactgcg gagaacctgg gcctgaccca gcctgccgtg
     6301 agcaatgcgc tgaaacgcct gcgcacctcg ctacaggacc cactcttcgt gcgcacacat
     6361 cagggaatgg aacccacacc ctatgccgcg catctggccg agcacgtcac ttcggccatg
     6421 cacgcactgc gcaacgccct acagcaccat gaaagcttcg atccgctgac cagcgagcgt
     6481 accttcaccc tggccatgac cgacattggc gagatctact tcatgccgcg gctgatggat
     6541 gcgctggctc accaggcccc caattgcgtg atcagtacgg tgcgcgacag ttcgatgagc
     6601 ctgatgcagg ccttgcagaa cggaaccgtg gacttggccg tgggcctgct tcccaatctg
     6661 caaactggct tctttcagcg ccggctgctc cagaatcact acgtgtgcct atgtcgcaag
     6721 gaccatccag tcacccgcga acccctgact ctggagcgct tctgttccta cggccacgtg
     6781 cgtgtcatcg ccgctggcac cggccacggc gaggtggaca cgtacatgac acgggtcggc
     6841 atccggcgcg acatccgtct ggaagtgccg cacttcgccg ccgttggcca catcctccag
     6901 cgcaccgatc tgctcgccac tgtgccgata tgtttagccg actgctgcgt agagcccttc
     6961 ggcctaagcg ccttgccgca cccagtcgtc ttgcctgaaa tagccatcaa catgttctgg
     7021 catgcgaagt accacaagga cctagccaat atttggttgc ggcaactgat gtttgacctg
     7081 tttacggatt gataaggatc ctaattggta acgaatcaga caattgacgg ctcgagggag
     7141 tagcataggg tttgcagaat ccctgcttcg tccatttgac aggcacatta tgcatcgatg
     7201 ataagctgtc aaacatgagc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     7261 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgat
//
