LOCUS       sequence-500886-        6482 bp    DNA     circular SYN 23-FEB-2026
DEFINITION  for ITC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6482)
  AUTHORS   Wickramaratne AC, Rasmussen ES, Chelliah Y, Schuhmann F, Solov'yov 
            IA, Mouritsen H, Green CB, Zoltowski BD, Takahashi JS
  TITLE     Structure of European robin cryptochrome 1 reveals a role in 
            circadian rhythms, not magnetoreception.
  JOURNAL   iScience. 2025 Nov 11;28(12):114015. doi: 
            10.1016/j.isci.2025.114015. eCollection 2025 Dec 19.
  PUBMED    41488359
REFERENCE   2  (bases 1 to 6482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 23, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2025-11-11"; volume: "28(12):114015. doi"; pages: " 
            10.1016/j.isci.2025.114015. eCollection 2025 Dec 19"
COMMENT     Sequence Label: erCRY1b full length
FEATURES             Location/Qualifiers
     source          1..6482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1905..1922
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2088..2222
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2125..2144)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2179..2198
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2251..2416
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2600..3054
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2687..2706)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2896..2917
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3080..3184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3185..3253,3254..4045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3403..3422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4216..4804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4705..4724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4958..4975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5109..5333
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5400..5933)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6103..6108,6109..6125,6126..6131))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atcccggtcc gaaatggggg tgaacgccgt gcactggttc cgcaaggggc
      181 tgcggctcca cgacaacccg gcgctgcggg aatgcatcca gggcgccgac acggtgcgct
      241 gcgtctacat cctggacccc tggttcgccg gctcctccaa cgtgggcatc aacaggtggc
      301 gattcctgct tcagtgtctt gaagatcttg atgccaatct acggaaactg aattcacgct
      361 tgtttgttat ccgtggacag ccagcagatg ttttccccag gctttttaag gaatggaaca
      421 ttgcaaagct ttctattgaa tatgattctg aaccatttgg gaaggagaga gatgcagcca
      481 tcaagaagct ggccagtgaa gctggagtgg aggtcattgt tcggatttct catacattgt
      541 atgacctaga caaaatcata gaattaaatg ggggacagcc tcctcttact tacaagcgat
      601 ttcagaccct aattagtaga atggaacccc tggagatgcc tgtggagact ataaccccag
      661 aagtaatgaa aaaatgtact actccagttt ctgatgacca tgatgagaaa tacggtgtgc
      721 catcacttga agagctgggc tttgacacag atggtctgcc ttctgcagta tggccagggg
      781 gagaaactga agctctcaca cgcttagaaa gacatttaga acgaaaggct tgggtagcaa
      841 actttgaaag accacggatg aatgcaaatt cccttctggc aagccctacg gggcttagtc
      901 cttacctccg cttcggctgt ctgtcctgcc ggctctttta tttcaagtta acggatctgt
      961 acaaaaaggt aaaaaagaac agctcccctc ccctctccct ctatggccag ctgttatggc
     1021 gtgaattttt ctacacagcg gcaactaaca atccgcggtt tgataaaatg gaggggaatc
     1081 ctatctgtgt tcaaatccca tgggataaga atcctgaggc tttggccaaa tgggcagaag
     1141 gcaggacagg ttttccttgg attgatgcaa ttatgacaca acttcgtcag gaaggttgga
     1201 ttcaccattt agcccggcat gctgtcgcat gctttttgac tcgaggtgac ctctggatta
     1261 gctgggaaga aggaatgaag gtctttgaag agctgttact tgatgcagat tggagtgtga
     1321 atgctggaag ctggatgtgg ctgtcctgta gttccttctt tcaacagttt ttccactgct
     1381 actgcccagt gggttttggc agaagaactg acccaaatgg ggattatatc agacggtatt
     1441 tgccagtact cagaggtttc cctgcaaaat acatctatga tccttggaat gccccagaga
     1501 gcatccagaa ggctgcaaaa tgtattatag gagttaatta tcccaaacca atggtaaacc
     1561 atgcagaggc aagccgtctg aatattgaaa ggatgaaaca gatctaccag cagctttcac
     1621 gatacagagg actgggtctt cttgcaactg tgccttctaa tccaaatgga aatggaaatg
     1681 gtggcctaat gggctattca ccaggagaaa gcatttctgg ttgtggtagt acaggaggag
     1741 ctcagctggg aactggtgat ggtcatactg ttgttcagtc atgtaccctg ggagactctc
     1801 attcaggaac aagtggaatt cagcagcaag gtattatggc agtgcctgtc tgtagaggat
     1861 ctccaaatgc ttgcaactat ggaaaaccag acaaaacatc aaaacaccat caccatcacc
     1921 attaatctag agcctgcagt ctcgaggcat gcggtaccaa gcttgtcgag aagtactaga
     1981 ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     2041 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     2101 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     2161 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     2221 tctgatcact gcttgagcct aggagatccg aaccagataa gtgaaatcta gttccaaact
     2281 attttgtcat ttttaatttt cgtattagct tacgacgcta cacccagttc ccatctattt
     2341 tgtcactctt ccctaaataa tccttaaaaa ctccatttcc acccctccca gttcccaact
     2401 attttgtccg cccacagcgg ggcatttttc ttcctgttat gtttttaatc aaacatcctg
     2461 ccaactccat gtgacaaacc gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat
     2521 ttttctgtca tctcttcgtt attaatgttt gtaattgact gaatatcaac gcttatttgc
     2581 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2641 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2701 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2761 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2821 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2881 tccacgttct taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     2941 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     3001 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     3061 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3121 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3421 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3721 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3901 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4141 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4321 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     4381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     4441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     4501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4861 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4921 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4981 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5041 agaccagccg cgtaacctgg caaaatcggt tacggttgag taataaatgg atgccctgcg
     5101 taagcgggtg tgggcggaca ataaagtctt aaactgaaca aaatagatct aaactatgac
     5161 aataaagtct taaactagac agaatagttg taaactgaaa tcagtccagt tatgctgtga
     5221 aaaagcatac tggacttttg ttatagctaa agcaaactct tcattttctg aagtgcaaat
     5281 tgcccgtcgt attaaagagg ggcgtggcca agggcatggt aaagactata ttcgcggcgt
     5341 tgtgacaatt taccgaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt
     5401 taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt
     5461 gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca
     5521 ccaagcgcgt tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc
     5581 cggtgctcgc cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac
     5641 ttgggcagaa cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc
     5701 gcgatgaatg tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg
     5761 gagtaggtgg ctacgtctcc gaactcacga ccgaaaagat caagagcagc ccgcatggat
     5821 ttgacttggt cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac
     5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
     5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa
     6001 cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag
     6061 gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag
     6121 gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc
     6181 ttgggcagca gcgaagtcga ggcatttctg tcctggctgg cgaacgagcg caaggtttcg
     6241 gtctccacgc atcgtcaggc attggcggcc ttgctgttct tctacggcaa ggtgctgtgc
     6301 acggatctgc cctggcttca ggagatcgga agacctcggc cgtcgcggcg cttgccggtg
     6361 gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc tggaaggcga gcatcgtttg
     6421 ttcgcccagg actctagcta tagttctagt ggttggctac gtatactccg gaatattaat
     6481 ag
//
