LOCUS       sequence-500915-        9382 bp    DNA     circular SYN 24-FEB-2026
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            (isoform 2) fused to COMP5AP-AviTag-9xHis. Protein is secreted from 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9382)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 24, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-KIR3DL3.2-COMP5AP-AviTag-9xHis
FEATURES             Location/Qualifiers
     source          1..9382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..143
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     149..168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      254..842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     743..762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     996..1013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1111..1128
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1112..1128
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        3105..3484
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3485..3688
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3639..3659
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3685..3709
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3919..3937
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3919..3936
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     3950..3969
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4987..5022
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5032..5055
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6715..6759
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6760..6786
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     primer_bind     complement(6926..6943)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6932..7043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7161..7181)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7166..7495
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7346..7481
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7408..7427
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7543..7590
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7609..8208
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7609..7628)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8105..8125
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8338..8459
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8375..8394)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8429..8448
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(8512..9303,9304..9372))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9135..9154
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9373..9382,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
       61 tgtatttaga aaaataaaca aataggggtc agtgttacaa ccaattaacc aattctgaac
      121 attatcgcga gcccatttat acctgaatat ggctcataac accccttgct catgaccaaa
      181 atcccttaac gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga
      241 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
      301 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      361 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
      421 tagcccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      481 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      541 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      601 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      661 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      721 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      781 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      841 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      901 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      961 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1021 aaggcgagag tagggaactg ccaggcatca aactaagcag aaggcccctg acggatggcc
     1081 tttttgcgtt tctacaaact ctttctgtgt tgtaaaacga cggccagtct taagctcggg
     1141 ccccctgggc ggttctgata acgagtaatc gttaatccgc aaataacgta aaaacccgct
     1201 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagaa tatttaaggg
     1261 cgcctgtcac tttgcttgat atatgagaat tatttaacct tataaatgag aaaaaagcaa
     1321 cgcactttaa ataagatacg ttgctttttc gattgatgaa cacctataat taaactattc
     1381 atctattatt tatgattttt tgtatataca atatttctag tttgttaaag agaattaaga
     1441 aaataaatct cgaaaataat aaagggaaaa tcagtttttg atatcaaaat tatacatgtc
     1501 aacgataata caaaatataa tacaaactat aagatgttat cagtatttat tatcatttag
     1561 aataaatttt gtgtcgccct taattgtgag cggataacaa ttacgagctt catgcacagt
     1621 ggcgttgact caatattggc cattagccat attattcatt ggttatatag cataaatcaa
     1681 tattggctat tggccattgc atacgttgta ctgatgaatc ccctaatgat ttttatcaaa
     1741 atcattaagt taaggtagat acacatcttg tcatatgatc aaatggtttc gccaaaaatc
     1801 aataatcaga caacaaaatg tgcgaactcg atattttaca cgactctctt taccaattct
     1861 gccccgaatt acacttaaaa cgactcaaca gcttaacgtt ggcttgccac gccttacttg
     1921 actgtaaaac tctcactctt accgaacttg gccgtaacct gccaaccaaa gcgagaacaa
     1981 aacataacat caaacgaatc gaccgattgt taggtaatcg tcacctccac aaagagcgac
     2041 tcgctgtata ccgttggcat gctagcttta tctgttcggg caatacgatg cccattgtac
     2101 ttgttgactg gtctgatatc cgtgagcaaa aacggcttat ggtattgcga gcttcagtcg
     2161 cactacacgg tcgttctgtt actctttatg agaaagcgtt cccgctttca gagcaatgtt
     2221 caaagaaagc tcatgaccaa tttctagccg accttgcgag cattctaccg agtaacacca
     2281 caccgctcat tgtcagtgat gctggcttta aagtgccatg gtataaatcc gttgagaagc
     2341 tgggttggta ctggttaagt cgagtaagag gaaaagtaca atatgcagac ctaggagcgg
     2401 aaaactggaa acctatcagc aacttacatg atatgtcatc tagtcactca aagactttag
     2461 gctataagag gctgactaaa agcaacccaa tctcatgcca aattctattg tataaatctc
     2521 gctctaaagg ccgaaaaaat cagcgctcga cacggactca ttgtcaccac ccgtcaccta
     2581 aaatctactc agcgtcggca aaggagccat ggattctagc aactaactta cctgttgaaa
     2641 ttcgaacacc caaacaactt gttaatatct attcgaagcg aatgcagatt gaagaaacct
     2701 tccgagactt gaaaagtcct gcctacggac taggcctacg ccatagccga acgagcagct
     2761 cagagcgttt tgatatcatg ctgctaatcg ccctgatgct tcaactaaca tgttggcttg
     2821 cgggcgttca tgctcagaaa caaggttggg acaagcactt ccaggctaac acagtcagaa
     2881 atcgaaacgt actctcaaca gttcgcttag gcatggaagt tttgcggcat tctggctaca
     2941 caataacaag ggaagactca ctcgtggctg caaccctgct tactcaaaat ctattcacac
     3001 atggttacgt tttggggaaa ttatgagggg atctctcagt acgttgtatc tatatcataa
     3061 tatgtacatt tatattggct catgtccaat atgaccgcca tgttggcatt gattattgac
     3121 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     3181 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     3241 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     3301 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     3361 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     3421 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3481 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     3541 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     3601 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     3661 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     3721 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccg
     3781 ggaacggtgc attggaacgc ggattccccg tgccaagagt gacgtaagta ccgcctatag
     3841 agtctatagg cccaccccct tggcttcgtt agaacgcggc tacaattaat acataacctt
     3901 atgtatcata cacatacgat ttaggtgaca ctatagaata acatccactt tgcctttctc
     3961 tccacaggtg tccactccca ggtccaactg cacctcggtt ctatcgaaaa cgcgcctgct
     4021 agcgccacca tgaagaccat cattgccctg agctacatct tctgcctggt gttcgctgcg
     4081 gccgcccaag ataagccttt tctgtctgct tggcctggca ccgtggtgtc tgaaggacag
     4141 catgtgaccc tgcagtgcag aagcagactg ggcttcaacg agttcagcct gagcaaagag
     4201 gacggcatgc ctgtgcctga gctgtacaac cggatcttcc ggaacagctt cctgatgggc
     4261 cctgtgacac cagctcacgc cggcacatac agatgctgta gctctcaccc tcacagcccc
     4321 acaggatggt ccgctccatc taaccctgtg gtcatcatgg tcaccggcgt gcacagaaag
     4381 ccttctctgc ttgcccatcc tggacctctg gtcaagtctg gcgagacagt gattctgcag
     4441 tgttggagcg acgtccgctt cgagagattc ctgctgcaca gagagggcat cacagaggac
     4501 cctctgagac tcgtgggaca gctgcatgat gccggcagcc aagtgaatta cagcatgggc
     4561 ccaatgacac ccgcactggc cggcacctat agatgtttcg gcagcgtgac ccatctgcct
     4621 tacgagctgt ctgcccctag cgaccctctg gatattgtgg ttgtgggcct gtacggcaag
     4681 cctagccttt ctgctcagcc tggacctaca gtgcaggccg gcgaaaatgt gacactgagc
     4741 tgttccagca gaagcctgtt cgacatctac cacctgtcca gagaagccga agccggcgaa
     4801 ctgagactga cagccgtgct gagagtgaac ggcaccttcc aggccaattt tccactggga
     4861 ccagtgacac acggcggcaa ttaccggtgc ttcggctctt ttagagccct gcctcatgct
     4921 tggagcgatc cctctgatcc tctgcctgtg tctgtgaccg gcaacagcag acaccttggc
     4981 gcgccggaag atcaagttga tccaagattg attgatggca agggcggatc tctggaggtg
     5041 ctgttccagg gaccaggcgg aagcggcaat cccggcggcg atctggcccc ccagatgctg
     5101 cgcgagctgc aggagaccaa tgccgccctg caggacgtgc gcgagctgct gcgccaccgc
     5161 gtgaaggaga tcaccttcct gaagaacacc gtgatggagt gcgacgcctg cggtatgcag
     5221 ccctctagag gctccggctc catcatccca gttgaggagg agaacccgga cttctggaac
     5281 cgcgaggcag ccgaggccct gggtgccgcc aagaagctgc agcctgcaca gacagccgcc
     5341 aagaacctca tcatcttcct gggcgatggg atgggggtgt ctacggtgac agctgccagg
     5401 atcctaaaag ggcagaagaa ggacaaactg gggcctgaga tacccctggc catggaccgc
     5461 ttcccatatg tggctctgtc caagacatac aatgtagaca aacatgtgcc agacagtgga
     5521 gccacagcca cggcctacct gtgcggggtc aagggcaact tccagaccat tggcttgagt
     5581 gcagccgccc gctttaacca gtgcaacacg acacgcggca acgaggtcat ctccgtgatg
     5641 aatcgggcca agaaagcagg gaagtcagtg ggagtggtaa ccaccacacg agtgcagcac
     5701 gcctcgccag ccggcaccta cgcccacacg gtgaaccgca actggtactc ggacgccgac
     5761 gtgcctgcct cggcccgcca ggaggggtgc caggacatcg ctacgcagct catctccaac
     5821 atggacattg acgtgatcct aggtggaggc cgaaagtaca tgtttcgcat gggaacccca
     5881 gaccctgagt acccagatga ctacagccaa ggtgggacca ggctggacgg gaagaatctg
     5941 gtgcaggaat ggctggcgaa gcgccagggt gcccggtatg tgtggaaccg cactgagctc
     6001 atgcaggctt ccctggaccc gtctgtgacc catctcatgg gtctctttga gcctggagac
     6061 atgaaatacg agatccaccg agactccaca ctggacccct ccctgatgga gatgacagag
     6121 gctgccctgc gcctgctgag caggaacccc cgcggcttct tcctcttcgt ggagggtggt
     6181 cgcatcgacc atggtcatca tgaaagcagg gcttaccggg cactgactga gacgatcatg
     6241 ttcgacgacg ccattgagag ggcgggccag ctcaccagcg aggaggacac gctgagcctc
     6301 gtcactgccg accactccca cgtcttctcc ttcggaggct accccctgcg agggagctcc
     6361 atcttcgggc tggcccctgg caaggcccgg gacaggaagg cctacacggt cctcctatac
     6421 ggaaacggtc caggctatgt gctcaaggac ggcgcccggc cggatgttac cgagagcgag
     6481 agcgggagcc ccgagtatcg gcagcagtca gcagtgcccc tggacgaaga gacccacgca
     6541 ggcgaggacg tggcggtgtt cgcgcgcggc ccgcaggcgc acctggttca cggcgtgcag
     6601 gagcagacct tcatagcgca cgtcatggcc ttcgccgcct gcctggagcc ctacaccgcc
     6661 tgcgacctgg cgccccccgc cggcaccacc gacgccgcgc acccgggtgg cggaggactg
     6721 aacgacatct tcgaggctca gaaaatcgaa tggcacgaac atcaccatca ccatcaccat
     6781 caccattaag gccggcctgt agtcactggc acaacgcgtt acccggtaag ccaatcgggt
     6841 atacacggtc gtcatactgc agacagggtt cttctacttt gcaagatagt cttgagtagt
     6901 aaaataaata gatagagaaa atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     6961 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7021 taataaaatg aggaaattgc atcacaacac tcaaccctat ctcggtctat tcttttgatt
     7081 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7141 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7201 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7261 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7321 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7381 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     7441 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     7501 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     7561 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat gaccgagtac
     7621 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     7681 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     7741 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     7801 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     7861 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     7921 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     7981 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     8041 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg
     8101 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     8161 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga
     8221 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8281 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8341 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8401 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8461 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc attaccaatg
     8521 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8581 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagcgctgc
     8641 gatgataccg cgagaaccac gctcaccggc tccggattta tcagcaataa accagccagc
     8701 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8761 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8821 catcgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8881 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8941 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     9001 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     9061 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     9121 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     9181 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9241 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9301 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9361 ttgaatactc atattcttcc tt
//
