LOCUS       sequence-501103-        8750 bp    DNA     circular SYN 25-FEB-2026
DEFINITION  Transient or stable lentiviral expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8750)
  AUTHORS   Fuller DM, Wu Y, Schueder F, Rasool B, Nag S, Korfhage JL, 
            Garcia-Milian R, Melnyk KD, Bewersdorf J, De Camilli P, Melia TJ
  TITLE     ATG2A engages RAB1A and ARFGAP1 positive membranes during 
            autophagosome biogenesis
  JOURNAL   eLife 2025 14:RP107316
REFERENCE   2  (bases 1 to 8750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife 2025 
            14:RP107316"
COMMENT     Sequence Label: pLVX puro GFP
FEATURES             Location/Qualifiers
     source          1..8750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(637..639,640..642,643..1356)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(637..639,640..642,643..1353)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(682..703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(943..962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1290..1311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1385..1884
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1420..1443)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1817..1836
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1905..2504
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1905..1924)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2401..2421
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2518..3106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2571..2591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2989..3000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3313..3946
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4075..4091)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4075..4091)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4088..4110)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4099..4115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4123..4129,4130..4147,4148..4153))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4168..4189
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4306..4323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4477..5065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4557..4576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5236..6027,6028..6096))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5859..5878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6097..6201)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6249..6383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6286..6305)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6340..6359
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6551..7184
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7231..7356
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7853..8086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8271..8315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8578..8693
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tcgctagcgc
      601 taccggactc agatctcgag ctcaagcttc gaattcatgg tgagcaaggg cgaggagctg
      661 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
      721 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      781 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
      841 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      901 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      961 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1021 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1081 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1141 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1201 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1261 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1321 gggatcactc tcggcatgga cgagctgtac aagtaaggat cccgcgactc tagataattc
     1381 taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg
     1441 ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt
     1501 aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta
     1561 gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta
     1621 gcacgtctca ctagtctcgt gcagagggac agccccgctg agcaatggag gggggtgggc
     1681 cttgggggcg gcggccaata gcagctttgc tccttcgctt tcggggctcg ggggcgggga
     1741 gggggggggc cggggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg
     1801 tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc
     1861 tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc
     1921 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     1981 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     2041 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     2101 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     2161 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     2221 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     2281 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     2341 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     2401 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     2461 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaccgcgt ctggaacaat
     2521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2881 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     2941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
     3121 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     3181 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     3241 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3301 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     3361 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     3421 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     3481 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     3541 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     3601 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     3661 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     3721 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     3781 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     3841 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     3901 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     3961 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
     4021 tgacattgct agcgttttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     4081 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4141 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4201 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4261 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     4321 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4381 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4441 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4501 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4561 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4621 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4681 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4741 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4801 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4861 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4921 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4981 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5041 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5101 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5161 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5221 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5281 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5341 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5401 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5461 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5521 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5581 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5641 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5701 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5761 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5821 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     5881 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     5941 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6001 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6061 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6121 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6181 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     6241 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     6301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     6361 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     6421 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     6481 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     6541 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     6601 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     6661 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     6721 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     6781 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     6841 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     6901 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     6961 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     7021 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7081 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7141 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7201 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7261 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7321 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7381 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7441 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7501 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7561 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7621 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     7681 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     7741 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     7801 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     7861 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     7921 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7981 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     8041 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     8101 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     8161 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     8221 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     8281 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     8341 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     8401 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     8461 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     8521 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt
     8581 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     8641 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     8701 tattacaggg acagcagaga tccagtttat cgataagctt gggagttccg
//
