LOCUS       sequence-501146-        8708 bp    DNA     circular SYN 25-FEB-2026
DEFINITION  Doxycycline-inducible 3xFLAG-tagged mouse HNRNPK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8708)
  TITLE     Formaldehyde-based RIP reveals correlated protein-RNA associations 
            and a requirement for HNRNPU in long-range Polycomb recruitment by 
            lncRNAs Airn and Kcnq1ot1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 25, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCL417_PB_miRE_tre_3xFLAG_mHNRNPK
FEATURES             Location/Qualifiers
     source          1..8708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1367..1681
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1375..1393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1411..1429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1446..1464
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1482..1500
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1518..1536
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1553..1571
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1589..1607
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1654..1678
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1720..1785
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    3205..3429
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3557..3647
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        3695..3906
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3919..4187
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             4224..5249
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    5278..5399
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5315..5334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5369..5388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   6162..6196
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6687..6703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6687..6703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6700..6722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6711..6727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6735..6741,6742..6759,6760..6765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6780..6801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6918..6935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7089..7677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7169..7188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7848..8639,8640..8708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8471..8490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagttcgagt ttactcccta
     1381 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgatgtcgag
     1441 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     1501 acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tatccctatc
     1561 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagg
     1621 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
     1681 ctggaccggt cgtttagtga accgtcagaa ttaaccatgg actacaaaga ccatgacggt
     1741 gattataaag atcatgacat cgattacaag gatgacgatg acaagagcgg atccatggag
     1801 accgaacagc cagaagaaac cttccccaac accgaaacca atggtgaatt tggtaaacgc
     1861 cctgcagaag atatggaaga ggagcaagcc tttaaaagat ctagaaatac tgatgagatg
     1921 gttgaattgc gcattttgct tcagagcaag aatgctggag cagtgattgg aaaaggaggc
     1981 aagaatatta aggctctccg tacagactac aatgccagtg tttcagtccc agacagcagt
     2041 ggccccgagc gcatactgag tatcagtgct gatattgaga cgattggaga aattctgaag
     2101 aaaatcatcc ctaccttgga agagggcctg cagttgccat cacccactgc aaccagccag
     2161 ctcccgctcg aatctgatgc tgtggaatgc ttaaattacc aacattataa aggaagtgac
     2221 tttgattgcg agttgagact gttgattcat cagagtctgg caggaggaat aattggtgtt
     2281 aaaggtgcta aaatcaaaga acttcgagaa aacactcaga caacaatcaa gcttttccag
     2341 gagtgctgcc ctcactctac tgacagagtt gttcttattg gaggaaaacc tgatagggtt
     2401 gtagaatgca tcaagatcat ccttgacctt atatctgagt ctcccatcaa aggacgtgca
     2461 caaccttatg atcccaactt ttatgatgag acctatgatt atggtggttt tacaatgatg
     2521 tttgatgacc gccgaggacg acctgtggga ttccccatga ggggaagagg tggttttgac
     2581 agaatgcctc ctggtcgggg tgggcgtccc atgcctcctt ctagaagaga ttatgatgat
     2641 atgagccctc gtcgaggacc tccaccacca ccacctggtc gaggtggcag gggtggcagc
     2701 agagcccgga atctgcctct tcctcctcca ccaccaccca gagggggaga tctaatggct
     2761 tatgacagaa gaggaaggcc tggagaccgc tatgatggca tggttgggtt cagtgctgat
     2821 gaaacttggg attctgcaat tgacacatgg agcccatcag aatggcaaat ggcttatgaa
     2881 ccacagggtg gttctggata tgactattct tatgcagggg gccgtggctc atatggtgat
     2941 cttggcggac ctattatcac tacacaagta actattccca aagatttggc tggatctatt
     3001 attggcaaag gtggtcagcg gattaaacaa attcgtcatg aatctggagc atcaatcaaa
     3061 attgatgaac ctttagaagg atctgaagat cggatcatta ccattacagg aacacaggac
     3121 cagatacaga acgcacagta tttgctgcag aacagtgtga agcagtattc tggaaagttt
     3181 ttctgagtcg acaatgcgtt tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3241 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3301 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3361 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3421 gctctatgga aacacgcggc gacgcgttcg accgaataaa acctgtgacg gaagatcact
     3481 tcgcagaata aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt
     3541 ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     3601 gatcactacc gggcgtattt tttgagttgt cgagattttc aggagctaaa gatctggaag
     3661 ctaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     3721 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg
     3781 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     3841 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     3901 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga
     3961 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga
     4021 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg
     4081 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct
     4141 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg
     4201 gcgcctacaa gctgctagcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga
     4261 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     4321 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     4381 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     4441 cgattccgga agtgcttgac attggggagt tcagcgagag cctgacctat tgcatctccc
     4501 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     4561 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     4621 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     4681 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     4741 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     4801 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     4861 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     4921 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     4981 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     5041 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     5101 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     5161 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     5221 ccagcactcg tccgagggca aaggaatagt tttacgtatt gagtatttaa ctaactaaac
     5281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5401 catgtctgga attgactcaa atgatgtcaa ttagtctatc agaagctcat ctggtctccc
     5461 ttccggggga caagacatcc ctgtttaata tttaaacagc agtgttccca aactgggttc
     5521 ttatatccct tgctctggtc aaccaggttg cagggtttcc tgtcctcaca ggaacgaagt
     5581 ccctaaagaa acagtggcag ccaggtttag ccccggaatt gactggattc cttttttagg
     5641 gcccattggt atggcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     5701 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     5761 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     5821 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     5881 tgtccctgat atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca
     5941 tgaaataaca atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca
     6001 tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga
     6061 caagcacgcc tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg
     6121 attcgcgcta tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca
     6181 gactatcttt ctagggttaa tctagctgca tcaggatcat atcgtcgggt cttttttccg
     6241 gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt
     6301 ttcccgcgag gttgaagcgg catggaaaga gtttgccgag gatgactgct gctgcattga
     6361 cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt
     6421 taacggtgaa ctgttcgttc aggccacctg ggataccagt tcgtcgcggc ttttccggac
     6481 acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg
     6541 gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag
     6601 cgaggacggg tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc
     6661 cggcgggcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6721 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6781 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6841 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6901 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6961 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7021 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7081 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7141 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7201 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7261 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7321 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7381 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7441 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7501 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7561 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7621 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7681 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7741 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7801 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7861 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7921 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7981 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8041 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8101 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8161 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8221 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8281 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8341 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8401 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8461 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8521 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8581 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8641 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8701 atactcat
//
