LOCUS       sequence-501237-        4299 bp    DNA     circular SYN 26-FEB-2026
DEFINITION  Donor Vector containing ZNF752 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Feb 26, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF752
FEATURES             Location/Qualifiers
     source          1..4299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             144..935
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1028..1616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1517..1536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1770..1787
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1946..1973)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2065..2151)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2200..2222
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2214..2231
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2215..2231
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2247..2346
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3856..3955)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3972..3991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3973..3991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3996..4012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3996..4012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
       61 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
      121 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
      181 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
      241 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
      301 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
      361 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
      421 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
      481 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
      541 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
      601 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
      661 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
      721 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
      781 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
      841 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
      901 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
      961 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1021 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1081 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1141 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1201 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1261 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1321 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1381 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1441 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1501 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1561 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1621 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1681 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1741 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1801 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1861 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta
     1921 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     1981 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     2041 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2101 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     2161 agttccctac tctcgcgtta acgctagcat ggatgttttc ccagtcacga cgttgtaaaa
     2221 cgacggccag tcttaagctc gggccccaaa taatgatttt attttgactg atagtgacct
     2281 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
     2341 ttggcaccat ggggactgag aacaaggagg tgattcccaa ggaagaaatt tctgaagaat
     2401 ctgagccaca tgggtcatta ttagaaaaat ttccaaaagt ggtttaccaa ggtcatgagt
     2461 ttggagcagg atgtgaagaa gacatgttgg agggacattc gagagagtcc atggaagagg
     2521 ttatagagca gatgtctcct caggagagag actttccatc agggttgatg atctttaaga
     2581 aatcaccctc aagtgagaaa gaccgggaga ataatgagag tgagagaggc tgcagtccca
     2641 gcccaaatct ggttacacat cagggagata caacagaggg agttagtgca tttgctacct
     2701 ctggccaaaa cttcctagag attttagaat ctaacaaaac acagagaagt tctgtgggag
     2761 aaaagcctca tacatgtaaa gaatgtggga aagcctttaa tcagaactca catctcatcc
     2821 agcatatgag agttcatagt ggagaaaaac cctttgaatg taaagaatgt ggaaagacat
     2881 ttggaactaa ttcaagcctt cgacggcacc tgagaattca tgctggagaa aaaccctttg
     2941 cttgtaatga atgtggaaag gccttcattc agagttcaca ccttattcac catcatagaa
     3001 ttcatactgg agagagaccc tataaatgtg aagaatgtgg taaagccttc agtcaaaatt
     3061 cagcccttat tctacaccag agaatccata ctggagagaa accatatgaa tgtaatgaat
     3121 gtgggaagac ctttagggtt agttcacagc ttattcagca tcagagaatt catactgaag
     3181 aaagatacca tgaatgcaat gagtgtggca aagccttcaa gcatagctca ggccttatta
     3241 gacaccagaa aattcatact ggagaaaaac catatctgtg taatgaatgt gggaagggct
     3301 tcgggcagag ttctgagctt atccggcatc agagaattca tacaggggac aaaccctatg
     3361 aatgtaatga atgtgggaaa acttttggcc agaactcaga gattattaga catattagaa
     3421 ttcatactgg tgagaagccc tatgtatgta aggaatgtgg gaaggccttc agggggaact
     3481 cagaacttct tagacatgag agaattcaca ctggagagaa accctatgaa tgctttgagt
     3541 gtggaaaggc tttcaggcgg acctctcacc ttattgtcca ccagagaatt catactggag
     3601 agaaacccca tcaatgtaat gagtgtgcaa gaaccttttg ggataattct gagctgcttc
     3661 tccaccagaa aattcatatt ggagagaaac cttatgaatg tagcgagtgt gagaaaacat
     3721 ttagccagca ttcccaactt atcatacatc agagaattca cactggagag aagccttatg
     3781 agtgccaaga atgtcagaag acttttagtc ggagctctca cctcctccga catcaaagtg
     3841 ttcactgtat ggagttgcca actttcttgt acaaagttgg cattataaga aagcattgct
     3901 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     3961 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     4021 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     4081 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     4141 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     4201 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     4261 gcagcggtgg taacggcgca gtggcggttt tcatggctt
//
