LOCUS       sequence-501404-        9786 bp    DNA     circular SYN 02-MAR-2026
DEFINITION  A PiggyBac donor vector for Dox-inducible expression of GATA3 and 
            membrane-localized tdTomato..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Oura S, Li L, Wu J
  TITLE     An inducible model of human post-implantation development derived 
            from primed and naive stem cells.
  JOURNAL   Cell Stem Cell. 2025 Oct 2;32(10):1509-1527.e9. doi: 
            10.1016/j.stem.2025.08.005. Epub 2025 Aug 29.
  PUBMED    40885193
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2025.08.005"; journalName: "Cell Stem Cell"; date: 
            "2025-10-2- 2"; volume: "32"; issue: "10"; pages: "1509-1527.e9"
COMMENT     Sequence Label: ePB-Puro_TT-hGATA3-P2A-tdTomato-CAAX
FEATURES             Location/Qualifiers
     source          1..9786
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(415..462)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(471..1682)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(615..634)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        1698..2012
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1706..1724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1742..1760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1777..1795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1813..1831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1849..1867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1884..1902
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1920..1938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1985..2009
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2039..2048
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3380..3436
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3446..4870
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     polyA_signal    5007..5128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5314..5376
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5411..5430)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5412..5430)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5440..5457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5440..5456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5449..5471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5598..6053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5685..5704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5895..5916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6079..6183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6184..6252,6253..7044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6402..6421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7215..7803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7704..7723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7957..7974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8091..8112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8127..8132,8133..8150,8151..8157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8165..8181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8170..8192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8189..8205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8189..8205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8224..8244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8226..8244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   8292..8326
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8614..8735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8782..9381)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8865..8885)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(9385..9438)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(9439..9444,9445..9480,9481..9483,9484..9519,
                     9520..9522,9523..9558,9559..9564,9565..9786,1..396))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag
       61 ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg
      121 tgagaggaga gcacagcgga atgacttggc gttgttccgc agaaagtctt gccatgactc
      181 gccttccagg gggcagaagt gggtatgatg cctgtccagc atctcgattg gcagggcatc
      241 gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt
      301 ttgagcgagt ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc
      361 gccgtttatg actttgctct tgtccagtct agacatggtt tagttcctca ccttgtcgta
      421 ttatactatg ccgatatact atgccgatga ttaattgtca acacggtccg gtctaacaaa
      481 aaagccaaaa acggccagaa tttagcggac aatttactag tctaacactg aaaattacat
      541 attgacccaa atgattacat ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc
      601 aaccccgagc gcatagttca aaaccggagc ttcagctact taagaagata ggtacataaa
      661 accgaccaaa gaaactgacg cctcacttat ccctcccctc accagaggtc cggcgcctgt
      721 cgattcagga gagcctaccc taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac
      781 cgcacaccta ccggcaggtg gccccaccct gcattataag ccaacagaac gggtgacgtc
      841 acgacacgac gagggcgcgc gctcccaaag gtacgggtgc actgcccaac ggcaccgcca
      901 taactgccgc ccccgcaaca gacgacaaac cgagttctcc agtcagtgac aaacttcacg
      961 tcagggtccc cagatggtgc cccagcccat ctcacccgaa taagagcttt cccgcattag
     1021 cgaaggcctc aagaccttgg gttcttgccg cccaccatgc cccccacctt gtttcaacga
     1081 cctcacagcc cgcctcacaa gcgtcttcca ttcaagactc gggaacagcc gccattttgc
     1141 tgcgctcccc ccaaccccca gttcagggca accttgctcg cggacccaga ctacagccct
     1201 tggcggtctc tccacacgct tccgtcccac cgagcggccc ggcggccacg aaagccccgg
     1261 ccagcccagc agcccgctac tcaccaagtg acgatcacag cgatccacaa acaagaaccg
     1321 cgacccaaat cccggctgcg acggaactag ctgtgccaca cccggcgcgt ccttatataa
     1381 tcatcggcgt tcaccgcccc acggagatcc ctccgcagaa tcgccgagaa gggactactt
     1441 ttcctcgcct gttccgctct ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg
     1501 tcctaaaatg tccttctgct gatactgggg ttctaaggcc gagtcttatg agcagcgggc
     1561 cgctgtcctg agcgtccggg cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt
     1621 ggcagcgctc gccgtgagga ggggggcgcc cgcggggggc gccaaaaccc ggcgcggagg
     1681 ccagatctcc cgatctcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     1741 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
     1801 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1861 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
     1921 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
     1981 gcagagctcg tttagtgaac cgtcagatcg cctggagaat tcgagctcgg tacccggggc
     2041 caccatggag gtgacggcgg accagccgcg ctgggtgagc caccaccacc ccgccgtgct
     2101 caacgggcag cacccggaca cgcaccaccc gggcctcagc cactcctaca tggacgcggc
     2161 gcagtacccg ctgccggagg aggtggatgt gctttttaac atcgacggtc aaggcaacca
     2221 cgtcccgccc tactacggaa actcggtcag ggccacggtg cagaggtacc ctccgaccca
     2281 ccacgggagc caggtgtgcc gcccgcctct gcttcatgga tccctaccct ggctggacgg
     2341 cggcaaagcc ctgggcagcc accacaccgc ctccccctgg aatctcagcc ccttctccaa
     2401 gacgtccatc caccacggct ccccggggcc cctctccgtc taccccccgg cctcgtcctc
     2461 ctccttgtcg gggggccacg ccagcccgca cctcttcacc ttcccgccca ccccgccgaa
     2521 ggacgtctcc ccggacccat cgctgtccac cccaggctcg gccggctcgg cccggcagga
     2581 cgagaaagag tgcctcaagt accaggtgcc cctgcccgac agcatgaagc tggagtcgtc
     2641 ccactcccgt ggcagcatga ccgccctggg tggagcctcc tcgtcgaccc accaccccat
     2701 caccacctac ccgccctacg tgcccgagta cagctccgga ctcttccccc ccagcagcct
     2761 gctgggcggc tcccccaccg gcttcggatg caagtccagg cccaaggccc ggtccagcac
     2821 agaaggcagg gagtgtgtga actgtggggc aacctcgacc ccactgtggc ggcgagatgg
     2881 cacgggacac tacctgtgca acgcctgcgg gctctatcac aaaatgaacg gacagaaccg
     2941 gcccctcatt aagcccaagc gaaggctgtc tgcagccagg agagcaggga cgtcctgtgc
     3001 gaactgtcag accaccacaa ccacactctg gaggaggaat gccaatgggg accctgtctg
     3061 caatgcctgt gggctctact acaagcttca caatattaac agacccctga ctatgaagaa
     3121 ggaaggcatc cagaccagaa accgaaaaat gtctagcaaa tccaaaaagt gcaaaaaagt
     3181 gcatgactca ctggaggact tccccaagaa cagctcgttt aacccggccg ccctctccag
     3241 acacatgtcc tccctgagcc acatctcgcc cttcagccac tccagccaca tgctgaccac
     3301 gcccacgccg atgcacccgc catccagcct gtcctttgga ccacaccacc cctccagcat
     3361 ggtcaccgcc atgggtgccg ccacgaactt ctctctgtta aagcaagcag gagacgtgga
     3421 agaaaacccc ggtcctatga cgcgtgtgag caagggcgag gaggtcatca aagagttcat
     3481 gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga
     3541 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg
     3601 ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta
     3661 cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa
     3721 gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc
     3781 cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga
     3841 cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     3901 ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca
     3961 ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta
     4021 ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     4081 acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca ccggcagcac
     4141 cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga
     4201 gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga
     4261 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     4321 gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa
     4381 ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg
     4441 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga
     4501 ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc
     4561 ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct
     4621 gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg
     4681 cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc
     4741 cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat
     4801 cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga
     4861 gctgtacaag aagcttagca aagatggtaa aaagaagaaa aagaagtcaa agacaaagtg
     4921 tgtaattatg tgagcggccg cgattaaaaa acctcccaca cctccccctg aacctgaaac
     4981 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     5041 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5101 gtttgtccaa actcatcaat gtatcttaag gaattcgata aaagttttgt tactttatag
     5161 aagaaatttt gagtttttgt ttttttttaa taaataaata aacataaata aattgtttgt
     5221 tgaatttatt attagtatgt aagtgtaaat ataataaaac ttaatatcta ttcaaattaa
     5281 taaataaacc tcgatataca gaccgataaa acacatgcgt caattttacg catgattatc
     5341 tttaacgtac gtcacaatat gattatcttt ctagggttaa tctagctgcg gcgcgccggt
     5401 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     5461 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5521 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5581 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5641 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5701 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5761 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5821 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5881 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5941 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     6001 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     6061 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     6121 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     6181 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6241 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6301 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6361 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6421 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     6481 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     6541 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6601 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6661 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6721 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6781 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6841 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6901 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6961 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     7021 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     7081 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7141 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7201 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7261 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7321 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7381 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7441 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7501 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7561 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7621 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7681 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7741 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7801 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7861 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7921 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7981 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     8041 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     8101 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     8161 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     8221 cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg ggcgcgccgc
     8281 agctagatta accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat
     8341 tgctctctct ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc
     8401 gcttggagct cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat
     8461 aacaaccgcg tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac
     8521 tcatacgata attatattgt tatttcgtgt tctacttacg tgataactta ttatatatat
     8581 attttcttgt tatagatggg aattcagaca tgataagata cattgatgag tttggacaaa
     8641 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     8701 tatttgtaac cattataagc tgcaataaac aagtttcgag gtcgagtgtc agtcctgctc
     8761 ctcggccacg aagtgcttag ctcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg
     8821 tccttcgggc acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     8881 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     8941 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     9001 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     9061 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     9121 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     9181 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     9241 ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc
     9301 gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac
     9361 cgtgggcttg tactcggcca tggtagggcc gggattctcc tccacgtcac cgcatgttag
     9421 aagacttcct ctgccctccc cggggagcat gtcaaggtca aaatcgtcaa gagcgtcagc
     9481 aggcagcata tcaaggtcaa agtcgtcaag ggcatcggct gggagcatgt ctaagtcaaa
     9541 atcgtcaagg gcgtcggccg gcccgccgct ttcgcacttt agctgtttct ccaggccaca
     9601 tatgattagt tccaggccga aaaggaaggc aggttcggct ccctgccggt cgaacagctc
     9661 aattgcttgt ctcagaagtg ggggcataga atcggtggta ggtgtctctc tttcctcttt
     9721 tgctacttga tgctcctgtt cctccaatac gcagcccagt gtaaagtggc ccacggcgga
     9781 cagagc
//
