LOCUS       sequence-501415-        9390 bp    DNA     circular SYN 02-MAR-2026
DEFINITION  A PiggyBac donor vector for Dox-inducible expression of CDX2 and 
            membrane-localized tdTomato..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9390)
  AUTHORS   Oura S, Li L, Wu J
  TITLE     An inducible model of human post-implantation development derived 
            from primed and naive stem cells.
  JOURNAL   Cell Stem Cell. 2025 Oct 2;32(10):1509-1527.e9. doi: 
            10.1016/j.stem.2025.08.005. Epub 2025 Aug 29.
  PUBMED    40885193
REFERENCE   2  (bases 1 to 9390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2025.08.005"; journalName: "Cell Stem Cell"; date: 
            "2025-10-2- 2"; volume: "32"; issue: "10"; pages: "1509-1527.e9"
COMMENT     Sequence Label: ePB-Puro_TT-hCDX2-P2A-tdTomato-CAAX
FEATURES             Location/Qualifiers
     source          1..9390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(415..462)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(471..1682)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(615..634)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        1698..2012
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1706..1724
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1742..1760
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1777..1795
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1813..1831
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1849..1867
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1884..1902
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1920..1938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1985..2009
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2984..3040
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3050..4474
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYE
                     GTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMN
                     FEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVL
                     KGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYER
                     SEGRHHLFLYGMDELYK"
     polyA_signal    4611..4732
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   4918..4980
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5015..5034)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5016..5034)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5044..5061)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5044..5060)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5053..5075)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5202..5657
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5289..5308)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5499..5520
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5683..5787
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5788..5856,5857..6648)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6006..6025)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6819..7407
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7308..7327
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7561..7578
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7695..7716
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7731..7736,7737..7754,7755..7761)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7769..7785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7774..7796
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7793..7809
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7793..7809
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7828..7848
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7830..7848
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   7896..7930
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    8218..8339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8386..8985)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MAEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8469..8489)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             complement(8989..9042)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(9043..9048,9049..9084,9085..9087,9088..9123,
                     9124..9126,9127..9162,9163..9168,9169..9390,1..396))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 gtacagtgcg ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag
       61 ggtttcgtac tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg
      121 tgagaggaga gcacagcgga atgacttggc gttgttccgc agaaagtctt gccatgactc
      181 gccttccagg gggcagaagt gggtatgatg cctgtccagc atctcgattg gcagggcatc
      241 gagcagggcc cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt
      301 ttgagcgagt ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc
      361 gccgtttatg actttgctct tgtccagtct agacatggtt tagttcctca ccttgtcgta
      421 ttatactatg ccgatatact atgccgatga ttaattgtca acacggtccg gtctaacaaa
      481 aaagccaaaa acggccagaa tttagcggac aatttactag tctaacactg aaaattacat
      541 attgacccaa atgattacat ttcaaaaggt gcctaaaaaa cttcacaaaa cacactcgcc
      601 aaccccgagc gcatagttca aaaccggagc ttcagctact taagaagata ggtacataaa
      661 accgaccaaa gaaactgacg cctcacttat ccctcccctc accagaggtc cggcgcctgt
      721 cgattcagga gagcctaccc taggcccgaa ccctgcgtcc tgcgacggag aaaagcctac
      781 cgcacaccta ccggcaggtg gccccaccct gcattataag ccaacagaac gggtgacgtc
      841 acgacacgac gagggcgcgc gctcccaaag gtacgggtgc actgcccaac ggcaccgcca
      901 taactgccgc ccccgcaaca gacgacaaac cgagttctcc agtcagtgac aaacttcacg
      961 tcagggtccc cagatggtgc cccagcccat ctcacccgaa taagagcttt cccgcattag
     1021 cgaaggcctc aagaccttgg gttcttgccg cccaccatgc cccccacctt gtttcaacga
     1081 cctcacagcc cgcctcacaa gcgtcttcca ttcaagactc gggaacagcc gccattttgc
     1141 tgcgctcccc ccaaccccca gttcagggca accttgctcg cggacccaga ctacagccct
     1201 tggcggtctc tccacacgct tccgtcccac cgagcggccc ggcggccacg aaagccccgg
     1261 ccagcccagc agcccgctac tcaccaagtg acgatcacag cgatccacaa acaagaaccg
     1321 cgacccaaat cccggctgcg acggaactag ctgtgccaca cccggcgcgt ccttatataa
     1381 tcatcggcgt tcaccgcccc acggagatcc ctccgcagaa tcgccgagaa gggactactt
     1441 ttcctcgcct gttccgctct ctggaaagaa aaccagtgcc ctagagtcac ccaagtcccg
     1501 tcctaaaatg tccttctgct gatactgggg ttctaaggcc gagtcttatg agcagcgggc
     1561 cgctgtcctg agcgtccggg cggaaggatc aggacgctcg ctgcgccctt cgtctgacgt
     1621 ggcagcgctc gccgtgagga ggggggcgcc cgcggggggc gccaaaaccc ggcgcggagg
     1681 ccagatctcc cgatctcgag tttactccct atcagtgata gagaacgtat gtcgagttta
     1741 ctccctatca gtgatagaga acgatgtcga gtttactccc tatcagtgat agagaacgta
     1801 tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg
     1861 atagagaacg tatgtcgagt ttatccctat cagtgataga gaacgtatgt cgagtttact
     1921 ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt acggtgggag gcctatataa
     1981 gcagagctcg tttagtgaac cgtcagatcg cctggagaat tcgagctcgg taccggccac
     2041 catgtacgtg agctacctcc tggacaagga cgtgagcatg taccctagct ccgtgcgcca
     2101 ctctggcggc ctcaacctgg cgccgcagaa cttcgtcagc cccccgcagt acccggacta
     2161 cggcggttac cacgtggcgg ccgcagctgc agcggcagcg aacttggaca gcgcgcagtc
     2221 cccggggcca tcctggccgg cagcgtatgg cgccccactc cgggaggact ggaatggcta
     2281 cgcgcccgga ggcgccgcgg ccgccgccaa cgccgtggct cacggcctca acggtggctc
     2341 cccggccgca gccatgggct acagcagccc cgcagactac catccgcacc accacccgca
     2401 tcaccacccg caccacccgg ccgccgcgcc ttcctgcgct tctgggctgc tgcaaacgct
     2461 caaccccggc cctcctgggc ccgccgccac cgctgccgcc gagcagctgt ctcccggcgg
     2521 ccagcggcgg aacctgtgcg agtggatgcg gaagccggcg cagcagtccc tcggcagcca
     2581 agtgaaaacc aggacgaaag acaaatatcg agtggtgtac acggaccacc agcggctgga
     2641 gctggagaag gagtttcact acagtcgcta catcaccatc cggaggaaag ccgagctagc
     2701 cgccacgctg gggctctctg agaggcaggt taaaatctgg tttcagaacc gcagagcaaa
     2761 ggagaggaaa atcaacaaga agaagttgca gcagcaacag cagcagcagc caccacagcc
     2821 gcctccgccg ccaccacagc ctccccagcc tcagccaggt cctctgagaa gtgtcccaga
     2881 gcccttgagt ccggtgtctt ccctgcaagc ctcagtgtct ggctctgtcc ctggggttct
     2941 ggggccaact gggggggtgc taaaccccac cgtcacccag gccgccacga acttctctct
     3001 gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct atgacgcgtg tgagcaaggg
     3061 cgaggaggtc atcaaagagt tcatgcgctt caaggtgcgc atggagggct ccatgaacgg
     3121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3181 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccccca
     3241 gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc gacatccccg attacaagaa
     3301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggtct
     3361 ggtgaccgtg acccaggact cctccctgca ggacggcacg ctgatctaca aggtgaagat
     3421 gcgcggcacc aacttccccc ccgacggccc cgtaatgcag aagaagacca tgggctggga
     3481 ggcctccacc gagcgcctgt acccccgcga cggcgtgctg aagggcgaga tccaccaggc
     3541 cctgaagctg aaggacggcg gccactacct ggtggagttc aagaccatct acatggccaa
     3601 gaagcccgtg caactgcccg gctactacta cgtggacacc aagctggaca tcacctccca
     3661 caacgaggac tacaccatcg tggaacagta cgagcgctcc gagggccgcc accacctgtt
     3721 cctggggcat ggcaccggca gcaccggcag cggcagctcc ggcaccgcct cctccgagga
     3781 caacaacatg gccgtcatca aagagttcat gcgcttcaag gtgcgcatgg agggctccat
     3841 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3901 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc
     3961 cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca tccccgatta
     4021 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     4081 cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga tctacaaggt
     4141 gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga agaccatggg
     4201 ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca
     4261 ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga ccatctacat
     4321 ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc tggacatcac
     4381 ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg gccgccacca
     4441 cctgttcctg tacggcatgg acgagctgta caagaagctt agcaaagatg gtaaaaagaa
     4501 gaaaaagaag tcaaagacaa agtgtgtaat tatgtgagcg gccgcgatta aaaaacctcc
     4561 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     4621 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4681 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggaattc
     4741 gataaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata
     4801 aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata
     4861 aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat
     4921 gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg
     4981 ttaatctagc tgcggcgcgc cggtacccaa ttcgccctat agtgagtcgt attacgcgcg
     5041 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     5101 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     5161 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     5221 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5281 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5341 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5401 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5461 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5521 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5581 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5641 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5701 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5761 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5821 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5881 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5941 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6001 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6061 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6121 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6181 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6241 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6301 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6361 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6421 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6481 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6541 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6601 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6661 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6721 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6781 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6841 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6901 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6961 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7021 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7081 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7141 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7201 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7261 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7321 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7381 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7441 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7501 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7561 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7621 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7681 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7741 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7801 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     7861 gagctccacc gcggggcgcg ccgcagctag attaacccta gaaagatagt ctgcgtaaaa
     7921 ttgacgcatg cattcttgaa atattgctct ctctttctaa atagcgcgaa tccgtcgctg
     7981 tgcatttagg acatctcagt cgccgcttgg agctcccgtg aggcgtgctt gtcaatgcgg
     8041 taagtgtcac tgattttgaa ctataacaac cgcgtgagtc aaaatgacgc atgattatct
     8101 tttacgtgac ttttaagatt taactcatac gataattata ttgttatttc gtgttctact
     8161 tacgtgataa cttattatat atatattttc ttgttataga tgggaattca gacatgataa
     8221 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     8281 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttt
     8341 cgaggtcgag tgtcagtcct gctcctcggc cacgaagtgc ttagctcagg caccgggctt
     8401 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     8461 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     8521 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     8581 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     8641 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     8701 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     8761 gaccgccacc gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     8821 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg ggtcgacggt
     8881 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg cccgggggac
     8941 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gccatggtag ggccgggatt
     9001 ctcctccacg tcaccgcatg ttagaagact tcctctgccc tccccgggga gcatgtcaag
     9061 gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc
     9121 ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gccggcccgc cgctttcgca
     9181 ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc
     9241 ggctccctgc cggtcgaaca gctcaattgc ttgtctcaga agtgggggca tagaatcggt
     9301 ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc
     9361 cagtgtaaag tggcccacgg cggacagagc
//
