LOCUS       sequence-501484-        9990 bp    DNA     circular SYN 02-MAR-2026
DEFINITION  Overexpression construct expressing OVOL2-3xFLAG-PURO and ORF-BC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9990)
  TITLE     Developmental and MAPK-responsive transcription factors regulate 
            distinct malignant cell states and associated genetic dependencies 
            in pancreatic cancer
REFERENCE   2  (bases 1 to 9990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Tet-O-FUW-OVOL2-3xFLAG-PURO
FEATURES             Location/Qualifiers
     source          1..9990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1211..1230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1402..1781
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1783..1981
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1999..2179
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2226..2351
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2844..3077
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3262..3306
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3604..3721
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(3778..3796,3797..3819,3820..3838,3839..3861,3862..3880,
                     3881..3903,3904..3922,3923..3945,3946..3964,3965..3987,
                     3988..4006,4007..4029,4030..4048)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        4081..4119
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4116..4140
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5051..5116
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5141..5197
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    5826..6414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5879..5899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6297..6308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6417..6433)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6418..6434)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6959..7139
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7165..7182)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7171..7395
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7441..7869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7528..7547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7738..7759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7878..7898)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7883..8212
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8063..8198
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8125..8144
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8260..8307
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8326..8700
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8830..8951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8867..8886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8921..8940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9000..9016)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9000..9016)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9013..9035)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9024..9040
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9048..9054,9055..9072,9073..9078))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9093..9114
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9231..9248)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9402..9990)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9482..9501)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     1201 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     1261 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     1321 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     1381 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     1441 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     1501 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     1561 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     1621 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     1681 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     1741 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     1801 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     1861 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     1921 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     1981 gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     2041 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     2101 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     2161 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     2221 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     2281 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     2341 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     2401 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     2461 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     2521 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     2581 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     2641 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     2701 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     2761 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     2821 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     2881 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     2941 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     3001 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     3061 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     3121 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     3181 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     3241 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     3301 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     3361 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     3421 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     3481 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     3541 ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca
     3601 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     3661 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     3721 tcgggtttat tacagggaca gcagagatcc agtttggtta gatctcgagt ttaccactcc
     3781 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     3841 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     3901 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     3961 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     4021 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagctcggta cccgggtcga
     4081 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
     4141 gcctggagac gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc
     4201 ctccgcggcc ccgaattccg ccaccatgcc caaagtcttc ctggtgaaga ggaggagcct
     4261 gggggtctcg gtccgcagct gggatgagct cccggatgag aaaagggcag acacctacat
     4321 cccagtgggc ctaggccgcc tgctccacga cccccccgag gactgccgca gcgacggcgg
     4381 cagcagcagc ggcagcggca gcagcagcgc gggggagcct ggaggagcag agagcagctc
     4441 gtccccgcac gcccccgaga gcgaaacccc cgagcccggc gacgccgagg gccccgatgg
     4501 acacctggcg accaagcagc gcccggtcgc cagatcgaaa atcaagttca ccacaggcac
     4561 gtgcagcgac tcggtggttc acagctgtga cctgtgtggc aagggcttcc gtctgcagcg
     4621 catgctgaac cgtcacctca agtgccacaa ccaggtgaaa agacacctgt gcaccttctg
     4681 cggcaagggc ttcaacgaca ccttcgacct gaagaggcac gtccgcacac acacaggcat
     4741 tcgtccctac aaatgcaacg tctgcaataa agccttcacc cagcgctgct ctctggagtc
     4801 ccacctgaag aaaatccatg gggtgcagca gcagtatgcc tataagcagc ggcgggacaa
     4861 gctctacgtc tgcgaggatt gcggctacac gggccccacc caggaggacc tgtacctgca
     4921 cgtgaacagt gcccatccgg gcagctcgtt tctcaaaaag acatctaaaa aactggcagc
     4981 ccttctgcag ggcaagctga catccgcaca ccaggagaat accagcctga gtgaggagga
     5041 ggagaggaag gattacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     5101 ggatgacgat gacaagacgc gtgaattcga tggatccggg gccaccaact tttccctgct
     5161 gaagcaggct ggcgacgtcg aggagaaccc tggccctggc tccggaaccg agtacaagcc
     5221 aactgtccgc ttggcaaccc gcgacgatgt gcccagggcc gtccgcacgc tggctgctgc
     5281 cttcgccgac tacccagcca cccgccacac cgtggacccg gaccgccaca tcgagcgcgt
     5341 caccgagctg caggagctct tcctcacccg agttggcctt gacattggca aggtctgggt
     5401 ggcggacgac ggtgctgccg tggccgtctg gaccaccccc gagtcggtcg aggctggcgc
     5461 cgtcttcgcc gagatcgggc cgcgcatggc cgagctttcg ggctcccggt tggctgccca
     5521 acagcagatg gaggggctgt tggcacccca ccgccccaag gagccggcct ggttcctggc
     5581 cacagttggt gtctctcccg accaccaggg caagggcctg ggcagcgccg tggtgctgcc
     5641 tggggtcgag gctgccgagc gcgctggcgt ccctgccttc ctggagacct ctgcccctcg
     5701 caacttgccc ttctacgagc gcctgggctt caccgtcacc gcggacgtcg aggtgcccga
     5761 ggggccgcgc acctggtgca tgacccgcaa gcctggggca tgaatctaga acgcgttaag
     5821 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     5881 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     5941 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6001 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6061 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6121 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6181 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     6241 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     6301 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     6361 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     6421 accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6481 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6541 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6601 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6661 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     6721 cagggatcag atatccactg acctttggat ggtgctacaa ggcggcctcc atcgaataga
     6781 actagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6841 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6901 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6961 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7021 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7081 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7141 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7201 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7261 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7321 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7381 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7441 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7501 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7561 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7621 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7681 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7741 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7801 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7861 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7981 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8041 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8101 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8161 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8221 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8281 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8341 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8401 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8461 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8521 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8581 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8641 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8701 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8761 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8821 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8941 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9001 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9061 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9121 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9181 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9241 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9301 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9361 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9421 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9481 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9541 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9601 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9661 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9721 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9781 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9841 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9901 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9961 cagattacgc gcagaaaaaa aggatctcaa
//
