LOCUS       sequence-501487-       10212 bp    DNA     circular SYN 02-MAR-2026
DEFINITION  Overexpression construct expressing ELF3-PURO and ORF-BC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10212)
  TITLE     Developmental and MAPK-responsive transcription factors regulate 
            distinct malignant cell states and associated genetic dependencies 
            in pancreatic cancer
REFERENCE   2  (bases 1 to 10212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 2, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Tet-O-FUW-ELF3-PURO
FEATURES             Location/Qualifiers
     source          1..10212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        135..182
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             201..575
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    705..826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(742..761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     796..815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(875..891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(875..891)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(888..910)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    899..915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(923..929,930..947,948..953))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    968..989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1106..1123)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1277..1865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1357..1376)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2036..2827,2828..2896))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2659..2678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2897..3001)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3076..3095)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3267..3646
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3648..3846
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             3864..4044
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4091..4216
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4709..4942
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5127..5171
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5469..5586
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(5643..5661,5662..5684,5685..5703,5704..5726,5727..5745,
                     5746..5768,5769..5787,5788..5810,5811..5829,5830..5852,
                     5853..5871,5872..5894,5895..5913)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5946..5984
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5981..6005
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6085..6094
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7228..7284
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    7913..8501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7966..7986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8384..8395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8504..8520)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8505..8521)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9046..9226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9252..9269)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9258..9482
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9528..9956
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9615..9634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9825..9846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9965..9985)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(9970..10212,1..87)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10150..10212,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     join(10212,1..19)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
      121 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
      181 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
      241 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
      301 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
      361 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
      421 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
      481 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
      541 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
      601 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
      661 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
      721 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      781 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
      841 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
      901 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
      961 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     1021 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     1081 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     1141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     1201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     1261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     1321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     1381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     1441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     1501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     1561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     1621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     1681 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     1741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     2161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     2221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     2281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     2341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     2401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     2461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     2521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     2581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     2641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3001 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct
     3061 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc
     3121 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca
     3181 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg
     3241 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
     3301 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3361 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3421 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3481 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3541 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3601 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     3661 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     3721 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     3781 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     3841 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3901 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3961 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     4021 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     4081 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     4141 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     4201 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     4261 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     4321 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     4381 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     4441 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     4501 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     4561 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     4621 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     4681 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     4741 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     4801 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     4861 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     4921 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     4981 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     5041 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     5101 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     5161 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     5221 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     5281 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     5341 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     5401 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     5461 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5521 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5581 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     5641 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5701 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     5761 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     5821 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5881 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     5941 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     6001 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     6061 ccagcctccg cggccccgaa ttccgccacc atggctgcaa cctgtgagat tagcaacatt
     6121 tttagcaact acttcagtgc gatgtacagc tcggaggact ccaccctggc ctctgttccc
     6181 cctgctgcca cctttggggc cgatgacttg gtactgaccc tgagcaaccc ccagatgtca
     6241 ttggagggta cagagaaggc cagctggttg ggggaacagc cccagttctg gtcgaagacg
     6301 caggttctgg actggatcag ctaccaagtg gagaagaaca agtacgacgc aagcgccatt
     6361 gacttctcac gatgtgacat ggatggcgcc accctctgca attgtgccct tgaggagctg
     6421 cgtctggtct ttgggcctct gggggaccaa ctccatgccc agctgcgaga cctcacttcc
     6481 agctcttctg atgagctcag ttggatcatt gagctgctgg agaaggatgg catggccttc
     6541 caggaggccc tagacccagg gccctttgac cagggcagcc cctttgccca ggagctgctg
     6601 gacgacggtc agcaagccag cccctaccac cccggcagct gtggcgcagg agccccctcc
     6661 cctggcagct ctgacgtctc caccgcaggg actggtgctt ctcggagctc ccactcctca
     6721 gactccggtg gaagtgacgt ggacctggat cccactgatg gcaagctctt ccccagcgat
     6781 ggttttcgtg actgcaagaa gggggatccc aagcacggga agcggaaacg aggccggccc
     6841 cgaaagctga gcaaagagta ctgggactgt ctcgagggca agaagagcaa gcacgcgccc
     6901 agaggcaccc acctgtggga gttcatccgg gacatcctca tccacccgga gctcaacgag
     6961 ggcctcatga agtgggagaa tcggcatgaa ggcgtcttca agttcctgcg ctccgaggct
     7021 gtggcccaac tatggggcca aaagaaaaag aacagcaaca tgacctacga gaagctgagc
     7081 cgggccatga ggtactacta caaacgggag atcctggaac gggtggatgg ccggcgactc
     7141 gtctacaagt ttggcaaaaa ctcaagcggc tggaaggagg aagaggttct ccagagtcgg
     7201 aacacgcgtg aattcgatgg atccggggcc accaactttt ccctgctgaa gcaggctggc
     7261 gacgtcgagg agaaccctgg ccctggctcc ggaaccgagt acaagccaac tgtccgcttg
     7321 gcaacccgcg acgatgtgcc cagggccgtc cgcacgctgg ctgctgcctt cgccgactac
     7381 ccagccaccc gccacaccgt ggacccggac cgccacatcg agcgcgtcac cgagctgcag
     7441 gagctcttcc tcacccgagt tggccttgac attggcaagg tctgggtggc ggacgacggt
     7501 gctgccgtgg ccgtctggac cacccccgag tcggtcgagg ctggcgccgt cttcgccgag
     7561 atcgggccgc gcatggccga gctttcgggc tcccggttgg ctgcccaaca gcagatggag
     7621 gggctgttgg caccccaccg ccccaaggag ccggcctggt tcctggccac agttggtgtc
     7681 tctcccgacc accagggcaa gggcctgggc agcgccgtgg tgctgcctgg ggtcgaggct
     7741 gccgagcgcg ctggcgtccc tgccttcctg gagacctctg cccctcgcaa cttgcccttc
     7801 tacgagcgcc tgggcttcac cgtcaccgcg gacgtcgagg tgcccgaggg gccgcgcacc
     7861 tggtgcatga cccgcaagcc tggggcatga atctagaacg cgttaagtcg acaatcaacc
     7921 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7981 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     8041 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     8101 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     8161 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     8221 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     8281 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     8341 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     8401 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     8461 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     8521 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
     8581 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     8641 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     8701 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     8761 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     8821 tccactgacc tttggatggt gctacaagcc tataggacat ccgaagaact agtaccagtt
     8881 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     8941 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     9001 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     9061 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9121 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9181 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     9241 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     9301 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9361 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     9421 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     9481 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     9541 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9601 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9661 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     9721 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     9781 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9841 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     9901 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     9961 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10021 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10081 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10141 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10201 ctaatttttt tt
//
