LOCUS       sequence-501530-        7337 bp    DNA     circular SYN 03-MAR-2026
DEFINITION  iATPSnFR2.A95A.A119L variant with non-responsive tdTomato.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7337)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 7337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 3, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.tdTomato
FEATURES             Location/Qualifiers
     source          1..7337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   61..190
                     /label=AAV2 ITR
     rep_origin      complement(427..1015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(507..526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1186..1977,1978..2046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1809..1828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2047..2151)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2177..2632)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2314..2335)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2526..2545
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2704..2833
                     /label=AAV2 ITR
     promoter        3146..3422
                     /label=chicken beta-actin promoter
     primer_bind     3701..3723
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3785..3804
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3838..3847
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     4395..4416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4519..4540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6484..7072
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6537..6557)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6955..6966)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7115..7236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7126..7145)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7180..7199
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
       61 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
      121 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
      181 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1621 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2161 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2221 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2281 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2341 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2401 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2461 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2521 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2581 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     2641 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2701 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
     2761 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     2821 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
     2881 tctaggaaga tcgtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2941 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3001 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3061 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3121 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     3181 atctcccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     3241 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     3301 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     3361 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     3421 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     3481 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     3541 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     3601 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg
     3661 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     3721 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     3781 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatcccgcc
     3841 accatgggca gatctatgaa gactattcac gtgagtgtcg taactcccga cgggcctgta
     3901 tatgaggatg acgttgaaat ggtgagcgtc aaagcaaaaa gtggcgagct cggtattctc
     3961 ccaggccaca ttcccttgaa agctccccta gagatcagtg ccgcacgcct gaagaaaggg
     4021 ggcaaaacac agtatatcgc tgtgtcaggc ggcaacctcg aagtgcggcc tgacaaggtg
     4081 accatctatg cccaggcagc cgagagggct gaggatatcg atgtcctgcg cgccaaggcc
     4141 gccaaggaga gagccgagcg ccgactccaa tcacaggtcc tggtcgggaa cgtctatatc
     4201 accgccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacgtggag
     4261 gacggcagca tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4321 gtgctgctgc ccgacaacca ctacctgagc acccagtccg tgctgagcaa agaccctaac
     4381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4441 atggacgagc tgtacaaggg cggtaccgga gggagcatga gcaagggcga ggagctgttc
     4501 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     4561 gtgcgcggcg agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc
     4621 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     4681 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4741 cccgaaggct acgtccagga gcgcaccatc agcttcaagg acgacggcac ctacaagacc
     4801 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     4861 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaactt tatttgtgtg
     4921 gacatcgatt ttaagcgcgc cgagctcttg cttaagcgcg caatgaatag gctctcagtt
     4981 gccgaaatga agctgcaggg cggaggctcg ggcggaggtg ggtcgggtgg cggcggatca
     5041 atggttagca agggtgaaga ggtgattaaa gagtttatgc gttttaaagt acgcatggag
     5101 ggtagcatga acggtcatga gtttgagatc gagggcgaag gcgaaggtcg tccgtacgag
     5161 ggtacgcaga ccgctaaact gaaagtaacc aaaggcggtc ctctgccgtt cgcgtgggac
     5221 atcctgtccc cgcagtttat gtatggttcc aaggcctacg tcaaacaccc agccgatatc
     5281 ccggactaca agaagctgtc tttcccggag ggtttcaagt gggagcgtgt aatgaacttt
     5341 gaggacggtg gcctggttac cgtcacccag gatagctctc tgcaagatgg caccctgatc
     5401 tacaaggtga agatgcgtgg tacgaacttt ccgccggatg gcccggtgat gcagaaaaag
     5461 acgatgggat gggaagcatc taccgagcgc ctgtatccgc gtgacggtgt tctgaaaggt
     5521 gagatccacc aggcgctgaa actgaaggac ggcggtcact acctggttga atttaagacg
     5581 atctatatgg ccaaaaagcc ggttcaactg ccaggttact actacgtgga caccaaactg
     5641 gatatcacgt ctcacaacga agattatacc atcgtcgaac agtatgaacg tagcgagggc
     5701 cgtcaccacc tgttcctggg ccacggtact ggttctacgg gttctggttc ctctggcact
     5761 gcgtcttccg aagacaacaa catggcggtg atcaaagagt tcatgaggtt caaagtacgt
     5821 atggaaggtt ctatgaacgg ccatgagttc gagattgaag gtgagggcga gggtcgccca
     5881 tacgagggca ctcagaccgc aaagctgaaa gttaccaaag gtggcccact gccttttgcg
     5941 tgggatatcc tgtctccgca gttcatgtac ggttccaaag cctacgtaaa acacccggca
     6001 gacatcccgg attacaaaaa gctgtccttc ccagagggct tcaaatggga acgtgttatg
     6061 aatttcgaag atggcggcct ggtcactgtt acgcaggaca gcagcctgca agacggtact
     6121 ctgatttaca aagtgaaaat gcgtggcact aacttcccgc ctgacggtcc agtaatgcag
     6181 aagaaaacta tgggctggga agctagcacc gaacgtctgt acccgcgcga cggtgtgctg
     6241 aaaggcgaga ttcaccaggc cctgaagctg aaggatggtg gtcactatct ggttgagttc
     6301 aaaactatct acatggcaaa aaagcctgta caactgccgg gctattacta cgttgacact
     6361 aaactggaca tcacctccca caacgaggat tatactattg ttgaacagta cgagcgttct
     6421 gaaggtcgcc atcatctgtt tctgtatggt atggacgaac tgtataaata gaagcttatc
     6481 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6541 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6601 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6661 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6721 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6781 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6841 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6901 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6961 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7021 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     7081 cgtcgacccg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     7141 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     7201 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     7261 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     7321 tgtggtaaaa tcgataa
//
