LOCUS       sequence-501566-        6320 bp    DNA     circular SYN 03-MAR-2026
DEFINITION  crystal structure.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Wickramaratne AC, Rasmussen ES, Chelliah Y, Schuhmann F, Solov'yov 
            IA, Mouritsen H, Green CB, Zoltowski BD, Takahashi JS
  TITLE     Structure of European robin cryptochrome 1 reveals a role in 
            circadian rhythms, not magnetoreception.
  JOURNAL   iScience. 2025 Nov 11;28(12):114015. doi: 
            10.1016/j.isci.2025.114015. eCollection 2025 Dec 19.
  PUBMED    41488359
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 3, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2025-11-11"; volume: "28(12):114015. doi"; pages: " 
            10.1016/j.isci.2025.114015. eCollection 2025 Dec 19"
COMMENT     Sequence Label: erCRY1b crystal
FEATURES             Location/Qualifiers
     source          1..6320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             159..176
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..218
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1926..2060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1963..1982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2017..2036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2089..2254
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2438..2892
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2525..2544)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2734..2755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2918..3022
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3023..3091,3092..3883)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3241..3260)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4054..4642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4543..4562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4796..4813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4947..5171
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5238..5771)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5941..5946,5947..5963,5964..5969))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaccatgt cgtactacca tcaccatcac catcacgatt
      181 acgatatccc aacgaccgaa aacctgtatt ttcagggcgc catgggatcc atgggggtga
      241 acgccgtgca ctggttccgc aaggggctgc ggctccacga caacccggcg ctgcgggaat
      301 gcatccaggg cgccgacacg gtgcgctgcg tctacatcct ggacccctgg ttcgccggct
      361 cctccaacgt gggcatcaac aggtggcgat tcctgcttca gtgtcttgaa gatcttgatg
      421 ccaatctacg gaaactgaat tcacgcttgt ttgttatccg tggacagcca gcagatgttt
      481 tccccaggct ttttaaggaa tggaacattg caaagctttc tattgaatat gattctgaac
      541 catttgggaa ggagagagat gcagccatca agaagctggc cagtgaagct ggagtggagg
      601 tcattgttcg gatttctcat acattgtatg acctagacaa aatcatagaa ttaaatgggg
      661 gacagcctcc tcttacttac aagcgatttc agaccctaat tagtagaatg gaacccctgg
      721 agatgcctgt ggagactata accccagaag taatgaaaaa atgtactact ccagtttctg
      781 atgaccatga tgagaaatac ggtgtgccat cacttgaaga gctgggcttt gacacagatg
      841 gtctgccttc tgcagtatgg ccagggggag aaactgaagc tctcacacgc ttagaaagac
      901 atttagaacg aaaggcttgg gtagcaaact ttgaaagacc acggatgaat gcaaattccc
      961 ttctggcaag ccctacgggg cttagtcctt acctccgctt cggctgtctg tcctgccggc
     1021 tcttttattt caagttaacg gatctgtaca aaaaggtaaa aaagaacagc tcccctcccc
     1081 tctccctcta tggccagctg ttatggcgtg aatttttcta cacagcggca actaacaatc
     1141 cgcggtttga taaaatggag gggaatccta tctgtgttca aatcccatgg gataagaatc
     1201 ctgaggcttt ggccaaatgg gcagaaggca ggacaggttt tccttggatt gatgcaatta
     1261 tgacacaact tcgtcaggaa ggttggattc accatttagc ccggcatgct gtcgcatgct
     1321 ttttgactcg aggtgacctc tggattagct gggaagaagg aatgaaggtc tttgaagagc
     1381 tgttacttga tgcagattgg agtgtgaatg ctggaagctg gatgtggctg tcctgtagtt
     1441 ccttctttca acagtttttc cactgctact gcccagtggg ttttggcaga agaactgacc
     1501 caaatgggga ttatatcaga cggtatttgc cagtactcag aggtttccct gcaaaataca
     1561 tctatgatcc ttggaatgcc ccagagagca tccagaaggc tgcaaaatgt attataggag
     1621 ttaattatcc caaaccaatg gtaaaccatg cagaggcaag ccgtctgaat attgaaagga
     1681 tgaaacagat ctaccagcag ctttcacgat acagaggact gggtcttctt gcaactgtgc
     1741 cttctaatcc aaatggaaat tagtctagag cctgcagtct cgaggcatgc ggtaccaagc
     1801 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     1861 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     1921 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1981 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2041 tatcttatca tgtctggatc tgatcactga tatcgcctag gagatccgaa ccagataagt
     2101 gaaatctagt tccaaactat tttgtcattt ttaattttcg tattagctta cgacgctaca
     2161 cccagttccc atctattttg tcactcttcc ctaaataatc cttaaaaact ccatttccac
     2221 ccctcccagt tcccaactat tttgtccgcc cacagcgggg catttttctt cctgttatgt
     2281 ttttaatcaa acatcctgcc aactccatgt gacaaaccgt catcttcggc tactttttct
     2341 ctgtcacaga atgaaaattt ttctgtcatc tcttcgttat taatgtttgt aattgactga
     2401 atatcaacgc ttatttgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     2461 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     2521 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     2581 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     2641 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     2701 cgccctttga cgttggagtc cacgttctta atagtggact cttgttccaa actggaacaa
     2761 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     2821 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     2881 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2941 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3001 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3061 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3121 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3181 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3241 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3301 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3361 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3421 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3481 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3541 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3601 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3661 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3721 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     3781 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     3841 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     3901 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     3961 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4021 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4081 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4141 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4201 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4261 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4321 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4381 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4441 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4501 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4561 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4621 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4681 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     4741 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     4801 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     4861 cggtatttca caccgcatag accagccgcg taacctggca aaatcggtta cggttgagta
     4921 ataaatggat gccctgcgta agcgggtgtg ggcggacaat aaagtcttaa actgaacaaa
     4981 atagatctaa actatgacaa taaagtctta aactagacag aatagttgta aactgaaatc
     5041 agtccagtta tgctgtgaaa aagcatactg gacttttgtt atggctaaag caaactcttc
     5101 attttctgaa gtgcaaattg cccgtcgtat taaagagggg cgtggccaag ggcatggtaa
     5161 agactatatt cgcggcgttg tgacaattta ccgaacaact ccgcggccgg gaagccgatc
     5221 tcggcttgaa cgaattgtta ggtggcggta cttgggtcga tatcaaagtg catcacttct
     5281 tcccgtatgc ccaactttgt atagagagcc actgcgggat cgtcaccgta atctgcttgc
     5341 acgtagatca cataagcacc aagcgcgttg gcctcatgct tgaggagatt gatgagcgcg
     5401 gtggcaatgc cctgcctccg gtgctcgccg gagactgcga gatcatagat atagatctca
     5461 ctacgcggct gctcaaactt gggcagaacg taagccgcga gagcgccaac aaccgcttct
     5521 tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga gcaagttccc gaggtaatcg
     5581 gagtccggct gatgttggga gtaggtggct acgtctccga actcacgacc gaaaagatca
     5641 agagcagccc gcatggattt gacttggtca gggccgagcc tacatgtgcg aatgatgccc
     5701 atacttgagc cacctaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg
     5761 ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc
     5821 atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     5881 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac
     5941 agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg tttccacggt
     6001 gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg
     6061 aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc
     6121 tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag acctcggccg
     6181 tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct cggttttctg
     6241 gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg ttggctacgt
     6301 atactccgga atattaatag
//
