LOCUS       sequence-501598-       10089 bp    DNA     circular SYN 05-MAR-2026
DEFINITION  express codon optimized PheS* gene with constitutive promoter 
            BBa_J23100.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1590 to 1624)
  AUTHORS   Lee C, Kim S
  TITLE     Continuous targeted hypermutation with a tunable mutation window.
  JOURNAL   Nat Commun. 2025 Nov 25. doi: 10.1038/s41467-025-66736-2.
  PUBMED    41290661
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 5, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun.
            2025 Nov 25. doi: 10.1038/s41467-025-66736-2."
COMMENT     Sequence Label: J23100_PheS*_codon
FEATURES             Location/Qualifiers
     source          1..10089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1164..1183
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1203..1280)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1486..1505
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        1590..1624
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     RBS             1639..1661
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1680..1697
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1707..1724
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             2718..2735
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2788..2806)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2802..2849
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3246..3719
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     CDS             complement(3792..4763)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(4763..5938)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    6264..6514
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(6517..7272)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      7363..7582
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      7669..8304
                     /label=oriV
                     /note="incP origin of replication"
     promoter        8928..9032
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9033..9101,9102..9893)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9251..9270)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cacggaaaaa ggttatgctg cttccaccgg aaggagctga ctgggttgaa ggctctcaag
       61 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
      121 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      181 cgcgcgggga gaggcggttt gcgtattggg cgccaggtgg tttttctttt caccagtgag
      241 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
      301 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
      361 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
      421 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
      481 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
      541 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
      601 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
      661 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
      721 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
      781 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
      841 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
      901 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
      961 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
     1021 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
     1081 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
     1141 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
     1201 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
     1261 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag
     1321 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
     1381 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
     1441 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
     1501 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
     1561 gaggatcgag atctcgatcc cgcgaaattt tgacggctag ctcagtccta ggtacagtgc
     1621 tagccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
     1681 atcatcatca tcatcacagc agcggcctgg tgccgcgcgg cagccatatg tcacatctcg
     1741 cagaactggt tgccagtgcg aaggcggcca ttagccaggc gtcagatgtt gccgcgttag
     1801 ataatgtgcg cgtcgaatat ttgggcaaga agggtcattt aacccttcag atgacgaccc
     1861 tgcgtgaact tccacctgat gaacgcccgg cagctggtgc agttatcaac gaagcgaaag
     1921 agcaggttca gcaggcgctg aatgcgcgta aagcggaact ggaaagcgct gcactgaatg
     1981 cgcgtctggc ggcggaaacg attgatgtct ctctgccagg tcgtcgcatt gaaaacggcg
     2041 gtctgcatcc ggttacccgt accatcgacc gtatcgaaag tttcttcggt gagcttggct
     2101 ttaccgtggc aaccgggccg gaaatcgaag acgattatca taacttcgat gctctgaaca
     2161 ttcctggtca ccacccggcg cgcgctgacc acgacacttt ctggtttgat accactcgtt
     2221 tgctgcgtac ccagacctct ggcgtacaga tccgcaccat gaaagcccag cagccaccga
     2281 tccgcattat tgcacctggc cgtgtttatc gtaacgacta cgaccagact cacaccccaa
     2341 tgtttcacca aatggaaggt ctgattgttg ataccaacat cagctttacc aacctgaaag
     2401 gcacgctgca cgacttcctg cgtaacttct ttgaggaaga tttgcagatt cgcttccgtc
     2461 cttcctactt cccgtttacc gaaccttctg cggaagtgga cgtcatgggt aaaaacggta
     2521 aatggctgga agtgctgggc tgcgggatgg tgcatccgaa cgtgttgcgt aacgttggca
     2581 tcgacccgga agtttactct ggtttcggct tcgggatggg gatggagcgt ctgactatgt
     2641 tgcgttacgg cgtcaccgac ctgcgttcat tcttcgaaaa cgatctgcgt ttcctcaaac
     2701 agtttaaata actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
     2761 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     2821 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatgaattc
     2881 caactgagcg ccggtcgcta ccattaccaa cttgtctggt gtcaaaaata ataggcctac
     2941 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3001 actcgacagc gacacacttg catcggatgc agtccggtta acgtgccggc acggcctggg
     3061 taaccaggta ttttgtccac ataaccgtgc gcaaaatgtt gtggataagc aggacacagc
     3121 agcaatccac agcaggcata caaccgcaca ccgaggttac tccgttctac aggttacgac
     3181 gacatgtcaa tacttgccct tgacaggcat tgatggaatc gtagtctcac gctgatagtc
     3241 tgatcgacaa tacaagtggg accgtggtcc cagaccgata atcagaccga caacacgagt
     3301 gggatcgtgg tcccagacta ataatcagac cgacgatacg agtgggaccg tggtcccaga
     3361 ctaataatca gaccgacgat acgagtggga cagtggttcc agactaataa tcagaccgac
     3421 gatacgagtg ggaccgtggt cccagactaa taatcagacc gacgatacga gtgggaccat
     3481 ggtcccagac taataatcag accgacgata cgagtgggac cgtggtccca gtctgattat
     3541 cagaccgacg atacgagtgg gaccgtggtc ccagactaat aatcagaccg acgatacgag
     3601 tgggaccgtg gtcccagact aataatcaga ccgacgatac gagtgggacc gtggtcccag
     3661 tctgattatc agaccgacga tacaagtgga acagtgggcc cagagagaat attcaggcca
     3721 gttatgcttt ctggcctgta acaaaggaca ttaagtaaag acagataaac gtagactaaa
     3781 acgtggtcgc atcagggtgc tggcttttca agttccttaa gaatggcctc aattttctct
     3841 atacactcag ttggaacacg agacctgtcc aggttaagca ccattttatc gcccttatac
     3901 aatactgtcg ctccaggagc aaactgatgt cgtgagctta aactagttct tgatgcagat
     3961 gacgttttaa gcacagaagt taaaagagtg ataacttctt cagcttcaaa tatcacccca
     4021 gcttttttct gctcatgaag gttagatgcc tgctgcttaa gtaattcctc tttatctgta
     4081 aaggcttttt gaagtgcatc acctgaccgg gcagatagtt caccggggtg agaaaaaaga
     4141 gcaacaactg atttaggcaa tttggcggtg ttgatacagc gggtaataat cttacgtgaa
     4201 atattttccg catcagccag cgcagaaata tttccagcaa attcattctg caatcggctt
     4261 gcataacgct gaccacgttc ataagcactt gttgggcgat aatcgttacc caatctggat
     4321 aatgcagcca tctgctcatc atccagctcg ccaaccagaa cacgataatc actttcggta
     4381 agtgcagcag ctttacgacg gcgactccca tcggcaattt ctatgacacc agatactctt
     4441 cgaccgaacg ccggtgtctg ttgaccagtc agtagaaaag aagggatgag atcatccagt
     4501 gcgtcctcag taagcagctc ctggtcacgt tcattacctg accatacccg agaggtcttc
     4561 tcaacactat caccccggag cacttcaaga gtaaacttca catcccgacc acatacaggc
     4621 aaagtaatgg cattaccgcg agccattact cctacgcgcg caattaacga atccaccatc
     4681 ggggcagctg gtgtcgataa cgaagtatct tcaaccggtt gagtattgag cgtatgtttt
     4741 ggaataacag gcgcacgctt cattatctaa tctcccagcg tggtttaatc agacgatcga
     4801 aaatttcatt gcagacaggt tcccaaatag aaagagcatt tctccaggca ccagttgaag
     4861 agcgttgatc aatggcctgt tcaaaaacag ttctcatccg gatctgacct ttaccaactt
     4921 catccgtttc acgtacaaca ttttttagaa ccatgcttcc ccaggcatcc cgaatttgct
     4981 cctccatcca cggggactga gagccattac tattgctgta tttggtaagc aaaatacgta
     5041 catcaggctc gaacccttta agatcaacgt tcttgagcag atcacgaagc atatcgaaaa
     5101 actgcagtgc ggaggtgtag tcaaacaact cagcaggcgt gggaacaatc agcacatcag
     5161 cagcacatac gacattaatc gtgccgatac ccaggttagg cgcgctgtca ataactatga
     5221 catcatagtc atgagcaaca gtttcaatgg ccagtcggag catcaggtgt ggatcggtgg
     5281 gcagtttacc ttcatcaaat ttgcccatta actcagtttc aatacggtgc agagccagac
     5341 aggaaggaat aatgtcaagc cccggccagc aagtgggctt tattgcataa gtgacatcgt
     5401 ccttttcccc aagatagaaa ggcaggagag tgtcttctgc atgaatatga agatctggta
     5461 cccatccgtg atacattgag gctgttccct gggggtcgtt accttccacg agcaaaacac
     5521 gtagcccctt cagagccaga tcctgagcaa gatgaacaga aactgaggtt ttgtaaacgc
     5581 cacctttatg ggcagcaacc ccgatcaccg gtggaaatac gtcttcagca cgtcgcaatc
     5641 gcgtaccaaa cacatcacgc atatgattaa tttgttcaat tgtataacca acacgttgct
     5701 caacccgtcc tcgaatttcc atatccgggt gcggtagtcg ccctgctttc tcggcatctc
     5761 tgatagcctg agaagaaacc ccaactaaat ccgctgcttc acctattctc cagcgccggg
     5821 ttattttcct cgcttccggg ctgtcatcat taaactgtgc aatggcgata gccttcgtca
     5881 tttcatgacc agcgtttatg cactggttaa gtgtttccat gagtttcatt ctgaacatcc
     5941 tttaatcatt gctttgcgtt tttttattaa atcttgcaat ttactgcaaa gcaacaacaa
     6001 aatcgcaaag tcatcaaaaa accgcaaagt tgtttaaaat aagagcaaca ctacaaaagg
     6061 agataagaag agcacatacc tcagtcactt attatcacta gcgctcgccg cagccgtgta
     6121 accgagcata gcgagcgaac tggcgaggaa gcaaagaaga actgttctgt cagatagctc
     6181 ttacgctcag cgcaagaaga aatatccacc gtgggaaaaa ctccaggtag aggtacacac
     6241 gcggatagcc aattcagagt aataaactgt gataatcaac cctcatcaat gatgacgaac
     6301 taacccccga tatcaggtca catgacgaag ggaaagagaa ggaaatcaac tgtgacaaac
     6361 tgccctcaaa tttggcttcc ttaaaaatta cagttcaaaa agtatgagaa aatccatgca
     6421 ggctgaagga aacagcaaaa ctgtgacaaa ttaccctcag taggtcagaa caaatgtgac
     6481 gaaccaccct caaatctgtg acagataacc ctcagactat cctgtcgtca tggaagtgat
     6541 atcgcggaag gaaaatacga tatgagtcgt ctggcggcct ttctttttct caatgtatga
     6601 gaggcgcatt ggagttctgc tgttgatctc attaacacag acctgcagga agcggcggcg
     6661 gaagtcaggc atacgctggt aactttgagg cagctggtaa cgctctatga tccagtcgat
     6721 tttcagagag acgatgcctg agccatccgg cttacgatac tgacacaggg attcgtataa
     6781 acgcatggca tacggattgg tgatttcttt tgtttcacta agccgaaact gcgtaaaccg
     6841 gttctgtaac ccgataaaga agggaatgag atatgggttg atatgtacac tgtaaagccc
     6901 tctggatgga ctgtgcgcac gtttgataaa ccaaggaaaa gattcatagc ctttttcatc
     6961 gccggcatcc tcttcagggc gataaaaaac cacttccttc cccgcgaaac tcttcaatgc
     7021 ctgccgtata tccttactgg cttccgcaga ggtcaatccg aatatttcag catatttagc
     7081 aacatggatc tcgcagatac cgtcatgttc ctgtagagtg ccatcagatt ttctgatctg
     7141 gtcaacgaac agatacagca tacgtttttg atcccgggag agactatatg ccgcctcagt
     7201 gaggtcgttt gactggacga ttcgcgggct atttttacgt ttcttgtgat tgataaccgc
     7261 tgtttccgcc atgacagatc catgtgaagt gtgacaagtt tttagattgt cacactaaat
     7321 aaaaaggagt caataagcag ggataacttt gtgaaaaaac agcttcttct gagggcaatt
     7381 tgtcacaggg ttaagggcaa tttgtcacag acaggactgt catttgaggg tgatttgtca
     7441 cactgaaagg gcaatttgtc acaacacctt ctctagaacc agcatggata aaggcctaca
     7501 aggcgctcta aaaaagaaga tctaaaaact ataaaaaaaa taattataaa aatatccccg
     7561 tggataagtg gataacccca agggaagttt tttcaggcat cgtgtgtaag cagaatatat
     7621 aagtgctgtt ccctggtgct tcctcgctca ctcgaaattc ccctttccga cgctcaccgg
     7681 gctggttgcc ctcgccgctg ggctggcggc cgtctatggc cctgcaaacg cgccagaaac
     7741 gccgtcgaag ccgtatgcga gacaccgcgg ccggccgccg gcgttgtgga tacctcgcgg
     7801 aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg gccgactcac
     7861 ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg tggagctggc
     7921 cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag atgatgtgga
     7981 caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact actgacagat
     8041 gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg gcgcacctat
     8101 tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt ttccgcccgt
     8161 ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt ataaaccttg
     8221 tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg ggtgcccccc
     8281 cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg gctgcgcccc
     8341 tcggccgcga acggcctcac cccaaaaatg gcaggggatc tcgagggctt cgccctgtcg
     8401 ctcgactgcg gcgagcacta ctggctgtaa aaggacagac cacatcatgg ttctgtgttc
     8461 attaggttgt tctgtccatt gctgacataa tccgctccac ttcaacgtaa caccgcacga
     8521 agatttctat tgttcctgaa ggcatattca aatcgttttc gttaccgctt gcaggcatca
     8581 tgacagaaca ctacttccta taaacgctac acaggctcct gagattaata atgcggatct
     8641 ctacgataat gggagatttt cccgactgtt tcgttcgctt ctcagtggat aacagccagc
     8701 ttctctgttt aacagacaaa aacagcatat ccactcagtt ccacatttcc atataaaggc
     8761 caaggcattt attctcagga taattgtttc agcatcgcaa ccgcatcaga ctccggcatc
     8821 gcaaactgca cccggtgccg ggcagccaca tccagcgcaa aaaccttcgt gtagacttcc
     8881 gttgaactga tggacttatg tcccatcagg ctttgcagaa ctttcagcgg taaaccccta
     8941 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     9001 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     9061 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     9121 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     9181 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     9241 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     9301 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     9361 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     9421 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     9481 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     9541 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     9601 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     9661 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     9721 ctgataaatc tggagccggt gagcgtggtt ctcgcggtat cattgcagca ctggggccag
     9781 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     9841 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     9901 accaagttta ctcaactgcg atgagtggca gggcggggcg taattttttt aaggcagtta
     9961 ttggtgccct taaacgcctg gttgctacgc ctgaataagt gataataagc ggatgaatgg
    10021 cagaaattcg atgataagct gtcaaacatg agaattggtc gagcatcttg gttaccgtga
    10081 agttaccat
//
