LOCUS       sequence-501627-       13749 bp    DNA     circular SYN 03-MAR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13749)
  AUTHORS   Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich 
            A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, 
            Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, 
            Cookson MR
  TITLE     Kinase activity is required for the toxic effects of mutant 
            LRRK2/dardarin.
  JOURNAL   Neurobiol Dis. 2006 Aug . 23(2):329-41.
  PUBMED    16750377
REFERENCE   2  (bases 1 to 13749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Mar 3, 2026 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2006 Aug . 23(2):329-41."
COMMENT     Sequence Label: pDEST53-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..13749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3991..4015)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4037..4747,4748..4750,4751..4753))
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(4077..4097)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4692..4720
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(4776..4795)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4777..4795)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4840..5043)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4869..4889)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5044..5423
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5595..5614
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5689..5793
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5794..5862,5863..6654)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6012..6031)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6825..7413
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7314..7333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7567..7584
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7701..7722
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7737..7742,7743..7760,7761..7767)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7775..7791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7780..7802
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7799..7815
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7799..7815
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7864..7985
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7875..7894)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7929..7948
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8159..8953)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8270..8289)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8880..8899
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(9020..9349)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9034..9169
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9088..9107)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9334..9354
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(9363..9791)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9473..9494)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9685..9704
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    9837..10061
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10050..10067
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     protein_bind    10099..10123
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atcgctgttg aagaaacctt atgatgtctt tggctttaca tcctatatgt ttaaaatcaa
       61 agttaagatg cagttcatcc aaaggaagat cccatatttt gcttaatttc cccatttcat
      121 tgggaaagga tcttagttcc aagttgtaac tgacatccag agatgtcaga ttttcaagac
      181 agccaatctc aggaggaatc tctttcagtt tattgtgaga aagatgcagt ttctctactc
      241 tagaccataa atatgctttt tcactcaagt ccaagatgct gatctgatta tggctaaata
      301 agagttccct taagttcaaa gatttccagt gtgcgggacc tggtaggtac tgaatatcat
      361 tgctgctcat atctaaagac cgcaagtgtg gaagatttaa aattgcttct ggaatacagg
      421 aaaatttgtt ctgagataat tttaggattg tcatagaagg aggcaagaaa ggcatagcag
      481 caagaaaatt cattctggca ctgaaactct ccactttagg acaagcctca agaaagttct
      541 ctgataggga tgaaatgtgg ttcttactaa ggtttaaaat cttcagttcc ttcagtctca
      601 agggggagca tatccctgat attttatttc cttctaaaat gagctgctcc agtttctcta
      661 ccacatcagt gaggttctca ggtacaaaag acagctggtt atatgacagg ttaaactgtt
      721 tcagagttgg acatttcact gtaggatcta aaaccactga gggtccaatg tcatttcgag
      781 agacatcaag attagcaata caactcattt tcaacaaata agaaggaaat gatgtaaatt
      841 tattactgtg caagtccaaa tgtgtcaaac tcttcagagt ttcacatagc tgttgtggaa
      901 agctcgtgag tgcattctgg tgaagctcca gcttttcaag atgctccaaa tgaacactta
      961 tacagcattt ctggcttagg gcatcaatat ctcttagttc atttgctgaa aggtctagtg
     1021 atgtaatata ttctctctca gaagccagag aagaaatgct gtctgaatgc ctcatatggg
     1081 attgaagttt tgatgacctg agtgaatcat ctgaagataa tatttttctt tttcgcttca
     1141 gtaaatcttc atgatcaaaa atgggcccca aggaattgga atgtctttgc aaatttggtg
     1201 agcaacgctg taatacggca tctcggtaaa attctcctac actaattgaa ttagatttct
     1261 ttttcacaag aaatgagcct tcacttcctt cactatccag gtcatcactt tgagcaaaca
     1321 cactgtccat agaagagtca ggaataaagg tccattcatc aaatttagac agcacatctt
     1381 cagaaaaatt tccatcgctg cctgaggctg ttccttcttc cacagcactt ttcatctgat
     1441 atctgatcac cattcttgct agtgtagatg ctatatttgt ttgtttcctt aaattagaag
     1501 tcttatctgg aaataaagga ccaagccaag aaggttcaac ttttcctata caaaatcctc
     1561 caaggcaaat gctattgttg gcaacatcca gggccagcct ccttaagagc aagctgatga
     1621 tctggctgtc acctttccca atgcttatcg tcaacgcttt tcgtacatct tgttcacgag
     1681 atccactatt cagtaagagt tccaccaatt tgggactgct ctctttctca catacctgac
     1741 aaattaaaga agatccctcc tttgcttgat tggcatctgc tcccaataga agcaagcatt
     1801 caaccatgat gctgttattc tgatcacacg ctctctctag catcacattt tttaagtaat
     1861 catccatagc tacttttgca aaacacttgc aacagaggtt tagaaactgt tgatcctttt
     1921 gttccatgat attggaagac atttgatgga atattactaa gtcaaatgaa tgatgcacca
     1981 gcagcttaga aaaagatgct gacaatttga ggattgctaa gattgtctga aatcctttag
     2041 tctgtatttc agcaacatcc ttaaatcggt ataagctgga aaccagaatt tttgccagca
     2101 gatgtccagt tcctatgaac acattcttct ttgtaatcaa gtatcctata agacttaaac
     2161 ccagacactg aatttcttgg tcatctggat acatctgcag tgtgtgaagc actgaatcca
     2221 tagcaccttc cagggataac atctctaatg catcaggaaa atgtacaata gaagaaatta
     2281 cttttaatcc acatttctga atcccaggat ttccaatgaa cctgttcaaa gctgctagga
     2341 ccagtttgtg aatatcattc ttgaaacact gttttttaac catatttagc ttatgatgaa
     2401 attctgtatc ctccctggat tcttctggca tgccaggcac tataaaatgt aaaatagctc
     2461 gaagcgcctc cagctgcact ggtaatgatg tctcatgacg tttcataact gttagtattt
     2521 tggggaccac tgctgccatt atatccaggg aagtgttgct tccttcaaaa agatgattta
     2581 gcattttaca gccactttca gccacttcgg gagaatgtat atgcttctgc attaactcca
     2641 aaacattcag gtgtattcct tttgataaca gtatttttct gaaattaaca ttttgttcta
     2701 agagagttga caatgcattc gcagatgcct ggaaaacttc ctttgatgaa gaatgcatca
     2761 gcatggagag catcacttcc ctatgagctg ggaaatggcc atcttcatct ccaatcttct
     2821 catgtaaact gttttggtac ataaggagat tatttagtgc ccagcatgcg gcctcctgca
     2881 cgtgcttgtt ctttctatgc cacgttaatg ctttgtaaca ggcttccagc caaaacaatt
     2941 tatcttcttc cccctcatca tcattctctt gattctcatt cttttcctct aaatcttgat
     3001 ttaagaaaat agtctcagtg aggagggcca aacagctgag cgctgagatc tgcaatgctg
     3061 cattctctgg gtactgctgc acagctttca ccacaaactc atggacttcg tttaatacca
     3121 ggatattgaa aaaattacct aatgtaagcc tatggagcaa acagcaactc acttcttgaa
     3181 ttctttcact catagggaat gctttcatag cttccaccac aatattataa cacctgacat
     3241 tgccactcat gaggacttcc acattattgc aaggaatcgc tagggaatgt aaacaatgca
     3301 gcacatgaag cacaatttcc tcttcatctt taaaatttgt taacgcactt aacaatatca
     3361 tataatcttt gttctcaaca aattcagtca gttgctcctc tgagactctc tcaaacagca
     3421 catgtaaagc tttgcatcca agtttctgga cttcatcatt ggctggaaat gagtgcatgg
     3481 catcaaaaat taacatgaaa atatcacttt cttcatccaa tatcagcaag gtgattttac
     3541 ctgaagttag gaggagatct aaggtcttca gtccaatcac tgacaagttt acactggcat
     3601 tatgaactgt tagcatttta agaatcaatt ggtgaacacc aaggacttcc caatcatttc
     3661 caacatcctg gggtcccatt aagctttgca ttgtacctgg acagacttct attaatttgc
     3721 acagaagtga ccaacccacc tgctgcacac tcgcgactct catataggag tccaagacga
     3781 tcaacagagg cacatggata tttttgcctt gaaataactt ggaggcgtgc tcggagtacg
     3841 tgaacaccag cagatcctcc aggatttgga ccagcgtttc tatctgtttt ccttcctgga
     3901 cattgttcag cctgactatc aacttcttca gagtttcctc gtcctcttcg cacccctgac
     3961 agctgccact agccataagg gcgaattcgg agcctgcttt tttgtacaaa cttgtttgat
     4021 ccggtccgga accgcttttg tagagctcat ccatgccatg tgtaatccca gcagcagtta
     4081 caaactcaag aaggaccatg tggtcacgct tttcgttggg atctttcgaa agggcagatt
     4141 gtgtcgacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt ggagtatttt
     4201 gttgataatg gtctgctagt tgaacggatc catcttcaat gttgtggcga attttgaagt
     4261 tagctttgat tccattcttt tgtttgtctg ccgtgatgta tacattgtgt gagttatagt
     4321 tgtactcgag tttgtgtccg agaatgtttc catcttcttt aaaatcaata ccttttaact
     4381 cgatacgatt aacaagggta tcaccttcaa acttgacttc agcacgcgtc ttgtagttcc
     4441 cgtcatcttt gaaagatata gtgcgttcct gtacataacc ttcgggcatg gcactcttga
     4501 aaaagtcatg ccgtttcata tgatccggat aacgggaaaa gcattgaaca ccataagaga
     4561 aagtagtgac aagtgttggc catggaacag gtagttttcc agtagtgcaa ataaatttaa
     4621 gggtaagctt tccgtatgta gcatcacctt caccctctcc actgacagaa aatttgtgcc
     4681 cattaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaaa agttcttctc
     4741 ctttgctggc catggtgtct agccagcttg ggtctcccta tagtgagtcg tattaatttc
     4801 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
     4861 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     4921 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     4981 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     5041 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     5101 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     5161 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     5221 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     5281 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     5341 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     5401 ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc gaagcagcgc
     5461 aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt gccttgttgt
     5521 agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag ggagcagata
     5581 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata ggggatcggg
     5641 agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5701 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5761 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5821 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5881 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5941 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6001 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6061 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6121 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6181 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6241 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6301 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6361 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6421 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6481 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6541 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6601 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6661 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6721 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6781 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6841 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6901 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6961 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7021 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7081 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7141 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7201 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7261 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7321 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7381 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7441 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7501 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7561 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7621 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7681 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7741 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7801 ggaaacagct atgaccatga ttacgccaag ctctagctag aggtcgacgg tatacagaca
     7861 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     7921 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     7981 aagttggggt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     8041 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     8101 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     8161 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     8221 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     8281 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     8341 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     8401 cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga
     8461 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     8521 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     8581 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     8641 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     8701 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     8761 ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     8821 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     8881 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     8941 cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatccg aaaatggata
     9001 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     9061 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     9121 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     9181 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     9241 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     9301 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     9361 ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     9421 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     9481 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     9541 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     9601 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     9661 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     9721 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     9781 acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat
     9841 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     9901 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     9961 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
    10021 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
    10081 ttatcttaat taattatcac cactttgtac aagaaagctg ggtcgaattc gcccttctct
    10141 cttactcaac agatgttcgt ctcatttttt cagctaattc ttttctcact tcaatgtgtt
    10201 tttctaaatt ttgcacttca tgtggaagat tgatgtccca aacggtcaag caagattgta
    10261 tctctttctg cttttgtgta ccttcagtat ttttccggtt gtagcccaat accagcatga
    10321 catttttaag gcttcctagc tgtgctgtca tcatgactct gaccgaatta caaaagttgt
    10381 aaattacacg tataagtcga cgagttgaaa gatccaggag taaaatatgg cctcctccag
    10441 ttcctatcca aagagcagtg ttcttctgaa ggcagagggt tttcactctc ccagaataag
    10501 acattttgtg ttttgattcc ttgttttctt ttaccattac ctcccttaaa aagtgcacgc
    10561 agtctattag tccacagagt ttttcagttt tcttatccca cacttccaca acagggctat
    10621 tttgcttagc aatatagaga gcagtgtcta ccaccactgt tatgatgttg gaatcactga
    10681 aagctgcata agaaaacagt tggcttgttc ttgtctcaat gagtttctga atggtgaaat
    10741 cattagaaaa ggagaaaatc tttgtgccac atcctcccca cattacattt ctttccgttg
    10801 aatttgtgga ttcactcaaa cacatcaatg gagtactgac atttcctata tttagtatct
    10861 tcaaaggagc agctccttta agcttaacag tcttatcttc aaaaattgct aacttgccat
    10921 cagcggttcc aaccaaaaga aaattttttt gtttgctttg cttggaaaag gaattgcaat
    10981 acaaacaagt gacagaatca gtcatctttt ctagggtatg tctctttttc ccatcttcgg
    11041 tattgatgac caggagagta ccagactgtg tcccagacac aatccagctt tccttttcaa
    11101 caggaagatg caccaaggct aagcacaata ttctactatc agcaacttcc tcagaagtgt
    11161 atccttcagt atttaagtca agaaatgaga gctgtcctct gtcggtgtgc ccacagccca
    11221 gccaaatgct tgcattcctg ctgttgtgat gtgtagcaac catgcattca acaattacgt
    11281 ttttaggtaa taaaatgcgt ctcgtcagac agactaattc agctgaattc aaaatgtcaa
    11341 agacctgggc agaagtaggc ctttcttgag gattttcttt caaacactgt ttaattaatt
    11401 tctcaaccat aggccatggg gcacaaccat attctttaac tggatcaggt aattttcctt
    11461 gtatttctaa ttcatcaaac tcatttggaa acttcaaacc ctctactatt ctacctccag
    11521 ttgtcaaaat gtcatagagt agtaaaccaa atgaataaac atcagcctgt tggttataaa
    11581 tgacatttcc tctggcaact tcaggtgcac gaaaccctgg tgtgccctct gatgttttta
    11641 tccccattct acagcagtac tgagcaatgc cgtagtcagc aatctttgcg atgatggcag
    11701 cattgggata cagtgtgaaa agcagcacat tgtggggttt caggtctcgg tatataatca
    11761 tggctgagtg gaggtatctc aaaccatcag ctacgtggag tgcgatcctg tgctgtaggg
    11821 ttctagtgag gctggctttg tcctgctgaa gcaggcgatc caaggaaccc ttggaggcta
    11881 actccatcac caacatccgg ggacgaatcc cagctgccag caaagatatc aaactggggt
    11941 ggtggaggtg gcaaagcacc acaagctctt gtcttaacag cctgagtgat gtatgtttat
    12001 taaaaatctt cacagccact tcttctcctt cataggctgc tcggtaaact gatccaaaac
    12061 tgccatcacc taggagaaac tctggagctt gttcaaattc caactcatca ttattcaaca
    12121 taatatttct aggcaggtca gccaaaatca agtcaggggc aatctgagat attggaatgg
    12181 tgagccttgg ttgatctgga tttactaaga gatctccttc ctctgctttc ttcatcaagt
    12241 catcaagtaa gattttttga tgttcttcgc catcattaaa actatataat gcccatttct
    12301 tcaacagagt ttctccttca ccacaaatat caatctccag caacccagga aaccattctt
    12361 ccatgagaga atcaatgtgg tccacaactt ggcccaaaag aatacagcct tttctacaag
    12421 aaggaactgt aatttttaag aaactctctg gatgattgtc taagacttca gatcctacca
    12481 gacaataagc ttcaggagac caatttaagt aaatgccttg tcgccaatac attctgtttg
    12541 ggcgaagtgc tcgttctctc cctgaaagca tgtaaggtga aatctcaagt aatcgattga
    12601 ttaatcttga ccaaaatccc attggaaaat aaggcatttc atatagtcgg atgataattt
    12661 cagagttctc acaatgggga agctctatca caggcctgtg gtcagacaaa ctgcttggaa
    12721 ccagcaaata ttcttctcct attggcaaag caatctggaa tttttctagg agcttaaaat
    12781 actgtgacat gtagttcttt ggaaatttcc ttttttttga aagaaatttt tccacatctc
    12841 tacgcgaaat aattccctta gggtgttttg gacaaccttc cactttcact gtcaaaatct
    12901 gtgccatgat tttacaaagc cacttgggtt ccacaaagta caagtcactt aactgcagtg
    12961 ctgggtcttg aaaatgaaga aggactcctg attcatttag aaagtgaact gcgtgaggaa
    13021 gctcattttc atctaactgc agctgatttt ctctcactag ttgtaataat cgtttccggt
    13081 caattacggg aaattcaatt ggcacatttt tacgctccga taaaatgatt ttttcaagtt
    13141 ctacatagca gtctggaatc agctgtccaa caacaagctg atctcggatc ttgaaattaa
    13201 ggctctcgtt tatgatggtt ttccgaagtt ttgccaaagc atcagattcc tcggtggcat
    13261 tcacaaagtg gtaatctcgt atggcaggga accctcgctt attcaggagt tccttggtga
    13321 ttttactcat gcaggctttg cgttgcttct catcagaaac atccaaatgt gtgccaacga
    13381 gaatcacagg ggaagaagaa gcgcgagcct ttatattgaa gagccaaggc ttcatggcat
    13441 caacttcagc ctgtcccttg ctgaggtcat agacagcaag gtacaatgct cgctgcgtca
    13501 taaaatgggg atgagtacta tagaattcct cacgacctgc aaaatcccac acatttagga
    13561 cgagatctct ctttcttttg tctcttattt ggataggcca gtctttcaca tctatgccaa
    13621 ctgtggcact ttgcattcca agatctgatt tcttggtttt cattaattgc tgcaataagg
    13681 tggttttacc actcccagta tttcccacaa tcataagttt cattcggtta taaggcacag
    13741 cctttttta
//
